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Cytokine responses in mice infected with Angiostrongylus cantonensis.
Parasitol Res. 1997; 83(1):10-5.PR

Abstract

For determination of the kinetics of cytokine production and its possible role in host resistance to Angiostrongylus cantonensis in the mouse, Th1 [interleukin 2 (IL-2) and interferon gamma (IFN-gamma] and Th2 (IL-5 and IL-4) cytokine production in the cerebrospinal fluid (CSF), sera, and culture supernatants of spleen cells (SC) or cervical lymph-node cells (CLNC) of infected BALB/c and C57BL/6 mice was assessed by a sandwich enzyme-linked immunosorbent assay (ELISA). IL-5 and IL-4 were detected in CSF of both strains, with a peak response occurring at around days 12-15 and 20 postinfection (p.i.), respectively. A reverse transcriptase-polymerase chain reaction (RT-PCR) assay also revealed prominent IL-5 and IL-4 mRNA expression in T-cells but not in eosinophils in CSF. SC and CLNC stimulated with A. cantonensis young adult-worm antigen released IL-5 in vitro at and after day 20 p.i. Contrarily, IFN-gamma production in CSF and SC or CLNC culture supernatants was almost negligible before day 30 p.i. IL-5, IL-4, and IL-2 production in culture supernatants was rather prominent in resistant C57BL/6 mice as opposed to susceptible BALB/c mice as assessed by the magnitude of increase over preinfection levels. Antigen-specific IgG1 (but not IgG2a) responses were more prominent in C57BL/6 mice than in BALB/c mice. These data suggest that systemic and local Th2 cytokine responses, especially those involving IL-5, are predominant in A. cantonensis-infected mice and that IL-5 is an important cytokine underlying the innate resistance of the mouse against A. cantonensis.

Authors+Show Affiliations

Department of Parasitology, Akita University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9000226

Citation

Sugaya, H, et al. "Cytokine Responses in Mice Infected With Angiostrongylus Cantonensis." Parasitology Research, vol. 83, no. 1, 1997, pp. 10-5.
Sugaya H, Aoki M, Abe T, et al. Cytokine responses in mice infected with Angiostrongylus cantonensis. Parasitol Res. 1997;83(1):10-5.
Sugaya, H., Aoki, M., Abe, T., Ishida, K., & Yoshimura, K. (1997). Cytokine responses in mice infected with Angiostrongylus cantonensis. Parasitology Research, 83(1), 10-5.
Sugaya H, et al. Cytokine Responses in Mice Infected With Angiostrongylus Cantonensis. Parasitol Res. 1997;83(1):10-5. PubMed PMID: 9000226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytokine responses in mice infected with Angiostrongylus cantonensis. AU - Sugaya,H, AU - Aoki,M, AU - Abe,T, AU - Ishida,K, AU - Yoshimura,K, PY - 1997/1/1/pubmed PY - 1997/1/1/medline PY - 1997/1/1/entrez SP - 10 EP - 5 JF - Parasitology research JO - Parasitol Res VL - 83 IS - 1 N2 - For determination of the kinetics of cytokine production and its possible role in host resistance to Angiostrongylus cantonensis in the mouse, Th1 [interleukin 2 (IL-2) and interferon gamma (IFN-gamma] and Th2 (IL-5 and IL-4) cytokine production in the cerebrospinal fluid (CSF), sera, and culture supernatants of spleen cells (SC) or cervical lymph-node cells (CLNC) of infected BALB/c and C57BL/6 mice was assessed by a sandwich enzyme-linked immunosorbent assay (ELISA). IL-5 and IL-4 were detected in CSF of both strains, with a peak response occurring at around days 12-15 and 20 postinfection (p.i.), respectively. A reverse transcriptase-polymerase chain reaction (RT-PCR) assay also revealed prominent IL-5 and IL-4 mRNA expression in T-cells but not in eosinophils in CSF. SC and CLNC stimulated with A. cantonensis young adult-worm antigen released IL-5 in vitro at and after day 20 p.i. Contrarily, IFN-gamma production in CSF and SC or CLNC culture supernatants was almost negligible before day 30 p.i. IL-5, IL-4, and IL-2 production in culture supernatants was rather prominent in resistant C57BL/6 mice as opposed to susceptible BALB/c mice as assessed by the magnitude of increase over preinfection levels. Antigen-specific IgG1 (but not IgG2a) responses were more prominent in C57BL/6 mice than in BALB/c mice. These data suggest that systemic and local Th2 cytokine responses, especially those involving IL-5, are predominant in A. cantonensis-infected mice and that IL-5 is an important cytokine underlying the innate resistance of the mouse against A. cantonensis. SN - 0932-0113 UR - https://www.unboundmedicine.com/medline/citation/9000226/Cytokine_responses_in_mice_infected_with_Angiostrongylus_cantonensis_ L2 - https://dx.doi.org/10.1007/s004360050199 DB - PRIME DP - Unbound Medicine ER -