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A specific elevation of RANTES in bronchoalveolar lavage fluids of patients with chronic eosinophilic pneumonia.
Lab Invest. 1997 Jan; 76(1):67-75.LI

Abstract

Chronic eosinophilic pneumonia (CEP) is a rare, idiopathic lung disorder characterized pathologically by massive eosinophil infiltration into lung. In the bronchoalveolar lavage fluid (BALF) of patients with CEP, eosinophil numbers were markedly increased but returned to normal-levels upon the resolution of clinical symptoms, which suggests the crucial role of eosinophils in the pathogenesis of CEP. To clarify the mechanism of eosinophil accumulation in CEP, we determined the BALF levels of RANTES and macrophage inflammatory protein-1 alpha, two chemokines that predominantly exhibit in vitro eosinophil chemotactic activities. RANTES (106.7 +/- 27.2 pg/mg albumin; n = 16) concentrations in BALF from patients with CEP were significantly elevated in comparison with those of normal control subjects (1.4 pg/mg albumin; n = 13), whereas BALF macrophage inflammatory protein-1 alpha levels were not. In addition, eosinophils, lymphocytes, and macrophages in BALF were positively stained with a specific anti-RANTES antibody, which suggests that RANTES was produced locally in the lungs of CEP patients. Moreover, BALF-RANTES levels correlated significantly with the proportion of eosinophils in BALF. Furthermore, nearly half of the eosinophil chemotactic activities in BALF were abrogated by the anti-RANTES antibody in vitro. Collectively, these data suggest that locally produced RANTES is involved in eosinophil accumulation in the pulmonary alveolus and interstitium of patients with CEP.

Authors+Show Affiliations

Third Department of Internal Medicine, School of Medicine, Kanazawa University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

9010450

Citation

Kurashima, K, et al. "A Specific Elevation of RANTES in Bronchoalveolar Lavage Fluids of Patients With Chronic Eosinophilic Pneumonia." Laboratory Investigation; a Journal of Technical Methods and Pathology, vol. 76, no. 1, 1997, pp. 67-75.
Kurashima K, Mukaida N, Fujimura M, et al. A specific elevation of RANTES in bronchoalveolar lavage fluids of patients with chronic eosinophilic pneumonia. Lab Invest. 1997;76(1):67-75.
Kurashima, K., Mukaida, N., Fujimura, M., Yasui, M., Shinagawa, T., Matsuda, T., Ohmoto, Y., & Matsushima, K. (1997). A specific elevation of RANTES in bronchoalveolar lavage fluids of patients with chronic eosinophilic pneumonia. Laboratory Investigation; a Journal of Technical Methods and Pathology, 76(1), 67-75.
Kurashima K, et al. A Specific Elevation of RANTES in Bronchoalveolar Lavage Fluids of Patients With Chronic Eosinophilic Pneumonia. Lab Invest. 1997;76(1):67-75. PubMed PMID: 9010450.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A specific elevation of RANTES in bronchoalveolar lavage fluids of patients with chronic eosinophilic pneumonia. AU - Kurashima,K, AU - Mukaida,N, AU - Fujimura,M, AU - Yasui,M, AU - Shinagawa,T, AU - Matsuda,T, AU - Ohmoto,Y, AU - Matsushima,K, PY - 1997/1/1/pubmed PY - 1997/1/1/medline PY - 1997/1/1/entrez SP - 67 EP - 75 JF - Laboratory investigation; a journal of technical methods and pathology JO - Lab Invest VL - 76 IS - 1 N2 - Chronic eosinophilic pneumonia (CEP) is a rare, idiopathic lung disorder characterized pathologically by massive eosinophil infiltration into lung. In the bronchoalveolar lavage fluid (BALF) of patients with CEP, eosinophil numbers were markedly increased but returned to normal-levels upon the resolution of clinical symptoms, which suggests the crucial role of eosinophils in the pathogenesis of CEP. To clarify the mechanism of eosinophil accumulation in CEP, we determined the BALF levels of RANTES and macrophage inflammatory protein-1 alpha, two chemokines that predominantly exhibit in vitro eosinophil chemotactic activities. RANTES (106.7 +/- 27.2 pg/mg albumin; n = 16) concentrations in BALF from patients with CEP were significantly elevated in comparison with those of normal control subjects (1.4 pg/mg albumin; n = 13), whereas BALF macrophage inflammatory protein-1 alpha levels were not. In addition, eosinophils, lymphocytes, and macrophages in BALF were positively stained with a specific anti-RANTES antibody, which suggests that RANTES was produced locally in the lungs of CEP patients. Moreover, BALF-RANTES levels correlated significantly with the proportion of eosinophils in BALF. Furthermore, nearly half of the eosinophil chemotactic activities in BALF were abrogated by the anti-RANTES antibody in vitro. Collectively, these data suggest that locally produced RANTES is involved in eosinophil accumulation in the pulmonary alveolus and interstitium of patients with CEP. SN - 0023-6837 UR - https://www.unboundmedicine.com/medline/citation/9010450/A_specific_elevation_of_RANTES_in_bronchoalveolar_lavage_fluids_of_patients_with_chronic_eosinophilic_pneumonia_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=9010450.ui DB - PRIME DP - Unbound Medicine ER -