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Effects of recombinant human growth hormone on muscle protein turnover in malnourished hemodialysis patients.
J Clin Invest 1997; 99(1):97-105JCI

Abstract

To assess the effect of recombinant human growth hormone (rhGH) on muscle protein metabolism in uremic patients with malnutrition, forearm [3H]phenylalanine kinetics were evaluated in six chronically wasted (body weight 79% of ideal weight) hemodialysis (HD) patients in a self-controlled, crossover study. Forearm protein dynamics were evaluated before, after a 6-wk course of rhGH (5 mg thrice weekly) and after a 6-wk washout period. After rhGH: (a) forearm phenylalanine net balance--the difference between phenylalanine incorporation into and phenylalanine release from muscle proteins--decreased by 46% (-8+/-2 vs. -15+/-2 nmol/min x 100 ml at the baseline and -11+/-2 after washout, P < 0.02); (b) phenylalanine rate of disposal, an index of protein synthesis, increased by 25% (25+/-5 vs. 20+/-5 at the baseline and 20+/-4 after washout, P < 0.03); (c) phenylalanine rate of appearance, an index of protein degradation, was unchanged (33+/-5 vs. 35+/-5 at the baseline and 31+/-4 after washout); (d) forearm potassium release declined (0.24+/-0.13 vs. 0.60+/-0.15 microeq/min at the baseline, and 0.42+/-0.20 microeq/min after washout P < 0.03); (e) changes in the insulin-like growth factor binding protein (IGFBP)-1 levels and insulin-like growth factor-I (IGF-I)/IGFBP-3 ratios accounted for 15.1% and 47.1% of the percent variations in forearm net phenylalanine balance, respectively. Together, these two factors accounted for 62.2% of variations in forearm net phenylalanine balance during and after rhGH administration. These data indicate: (a) that rhGH administration in malnourished hemodialysis patients is followed by an increase in muscle protein synthesis and by a decrease in the negative muscle protein balance observed in the postabsorptive state; and (b) that the reduction in net protein catabolism obtained with rhGH can be accounted for by the associated changes in circulating free, but not total, IGF-I levels.

Authors+Show Affiliations

Department of Internal Medicine, University of Genoa, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9011582

Citation

Garibotto, G, et al. "Effects of Recombinant Human Growth Hormone On Muscle Protein Turnover in Malnourished Hemodialysis Patients." The Journal of Clinical Investigation, vol. 99, no. 1, 1997, pp. 97-105.
Garibotto G, Barreca A, Russo R, et al. Effects of recombinant human growth hormone on muscle protein turnover in malnourished hemodialysis patients. J Clin Invest. 1997;99(1):97-105.
Garibotto, G., Barreca, A., Russo, R., Sofia, A., Araghi, P., Cesarone, A., ... Tizianello, A. (1997). Effects of recombinant human growth hormone on muscle protein turnover in malnourished hemodialysis patients. The Journal of Clinical Investigation, 99(1), pp. 97-105.
Garibotto G, et al. Effects of Recombinant Human Growth Hormone On Muscle Protein Turnover in Malnourished Hemodialysis Patients. J Clin Invest. 1997 Jan 1;99(1):97-105. PubMed PMID: 9011582.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of recombinant human growth hormone on muscle protein turnover in malnourished hemodialysis patients. AU - Garibotto,G, AU - Barreca,A, AU - Russo,R, AU - Sofia,A, AU - Araghi,P, AU - Cesarone,A, AU - Malaspina,M, AU - Fiorini,F, AU - Minuto,F, AU - Tizianello,A, PY - 1997/1/1/pubmed PY - 1997/1/1/medline PY - 1997/1/1/entrez SP - 97 EP - 105 JF - The Journal of clinical investigation JO - J. Clin. Invest. VL - 99 IS - 1 N2 - To assess the effect of recombinant human growth hormone (rhGH) on muscle protein metabolism in uremic patients with malnutrition, forearm [3H]phenylalanine kinetics were evaluated in six chronically wasted (body weight 79% of ideal weight) hemodialysis (HD) patients in a self-controlled, crossover study. Forearm protein dynamics were evaluated before, after a 6-wk course of rhGH (5 mg thrice weekly) and after a 6-wk washout period. After rhGH: (a) forearm phenylalanine net balance--the difference between phenylalanine incorporation into and phenylalanine release from muscle proteins--decreased by 46% (-8+/-2 vs. -15+/-2 nmol/min x 100 ml at the baseline and -11+/-2 after washout, P < 0.02); (b) phenylalanine rate of disposal, an index of protein synthesis, increased by 25% (25+/-5 vs. 20+/-5 at the baseline and 20+/-4 after washout, P < 0.03); (c) phenylalanine rate of appearance, an index of protein degradation, was unchanged (33+/-5 vs. 35+/-5 at the baseline and 31+/-4 after washout); (d) forearm potassium release declined (0.24+/-0.13 vs. 0.60+/-0.15 microeq/min at the baseline, and 0.42+/-0.20 microeq/min after washout P < 0.03); (e) changes in the insulin-like growth factor binding protein (IGFBP)-1 levels and insulin-like growth factor-I (IGF-I)/IGFBP-3 ratios accounted for 15.1% and 47.1% of the percent variations in forearm net phenylalanine balance, respectively. Together, these two factors accounted for 62.2% of variations in forearm net phenylalanine balance during and after rhGH administration. These data indicate: (a) that rhGH administration in malnourished hemodialysis patients is followed by an increase in muscle protein synthesis and by a decrease in the negative muscle protein balance observed in the postabsorptive state; and (b) that the reduction in net protein catabolism obtained with rhGH can be accounted for by the associated changes in circulating free, but not total, IGF-I levels. SN - 0021-9738 UR - https://www.unboundmedicine.com/medline/citation/9011582/Effects_of_recombinant_human_growth_hormone_on_muscle_protein_turnover_in_malnourished_hemodialysis_patients_ L2 - https://doi.org/10.1172/JCI119139 DB - PRIME DP - Unbound Medicine ER -