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Hook-length control of the export-switching machinery involves a double-locked gate in Salmonella typhimurium flagellar morphogenesis.
J Bacteriol. 1997 Feb; 179(4):1268-73.JB

Abstract

During flagellar morphogenesis in Salmonella typhimurium, the genes involved in filament assembly are expressed fully only after completion of hook-basal body assembly. This coupling of gene expression to morphogenesis is achieved by exporting the flagellum-specific anti-sigma factor, FlgM, out of the cell through the mature hook-basal body structure. Therefore, the flagellum-specific export apparatus must be able to sense the assembly state of the flagellar structure and to turn on FlgM export at a specific stage of hook assembly. It has been suggested that FlhB may act as the molecular switch which mediates this ordered export. Here, I report genetic evidence that in addition to FlhB, the product of a newly identified gene, rflH, is involved in the negative regulation of FlgM export. FlgM is released through the basal body structure lacking the hook and the filament only when the flhB and rflH genes are both defective. Therefore, the export gate for FlgM should be double locked by FlhB and RflH. The rflH gene is located at around 52 min, where no flagellum-related gene has been found. I propose a revised model of the export-switching machinery which consists of two systems, the hook-length signal transduction pathway and the double-locked gate for FlgM export.

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Authors+Show Affiliations

Kutsukake K
Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Japan.

MeSH

Bacterial ProteinsBiological TransportChromosome MappingFlagellaGenes, BacterialGenetic Complementation TestMembrane ProteinsMorphogenesisMutationSalmonella typhimuriumSignal TransductionTransduction, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9023211

Citation

Kutsukake, K. "Hook-length Control of the Export-switching Machinery Involves a Double-locked Gate in Salmonella Typhimurium Flagellar Morphogenesis." Journal of Bacteriology, vol. 179, no. 4, 1997, pp. 1268-73.
Kutsukake K. Hook-length control of the export-switching machinery involves a double-locked gate in Salmonella typhimurium flagellar morphogenesis. J Bacteriol. 1997;179(4):1268-73.
Kutsukake, K. (1997). Hook-length control of the export-switching machinery involves a double-locked gate in Salmonella typhimurium flagellar morphogenesis. Journal of Bacteriology, 179(4), 1268-73.
Kutsukake K. Hook-length Control of the Export-switching Machinery Involves a Double-locked Gate in Salmonella Typhimurium Flagellar Morphogenesis. J Bacteriol. 1997;179(4):1268-73. PubMed PMID: 9023211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hook-length control of the export-switching machinery involves a double-locked gate in Salmonella typhimurium flagellar morphogenesis. A1 - Kutsukake,K, PY - 1997/2/1/pubmed PY - 1997/2/1/medline PY - 1997/2/1/entrez SP - 1268 EP - 73 JF - Journal of bacteriology JO - J Bacteriol VL - 179 IS - 4 N2 - During flagellar morphogenesis in Salmonella typhimurium, the genes involved in filament assembly are expressed fully only after completion of hook-basal body assembly. This coupling of gene expression to morphogenesis is achieved by exporting the flagellum-specific anti-sigma factor, FlgM, out of the cell through the mature hook-basal body structure. Therefore, the flagellum-specific export apparatus must be able to sense the assembly state of the flagellar structure and to turn on FlgM export at a specific stage of hook assembly. It has been suggested that FlhB may act as the molecular switch which mediates this ordered export. Here, I report genetic evidence that in addition to FlhB, the product of a newly identified gene, rflH, is involved in the negative regulation of FlgM export. FlgM is released through the basal body structure lacking the hook and the filament only when the flhB and rflH genes are both defective. Therefore, the export gate for FlgM should be double locked by FlhB and RflH. The rflH gene is located at around 52 min, where no flagellum-related gene has been found. I propose a revised model of the export-switching machinery which consists of two systems, the hook-length signal transduction pathway and the double-locked gate for FlgM export. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/9023211/Hook_length_control_of_the_export_switching_machinery_involves_a_double_locked_gate_in_Salmonella_typhimurium_flagellar_morphogenesis_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=9023211 DB - PRIME DP - Unbound Medicine ER -