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Detection of antibodies to Coccidioides immitis by enzyme immunoassay.
Am J Clin Pathol. 1997 Feb; 107(2):148-53.AJ

Abstract

Two hundred twenty-six specimens (160 serum samples, 66 cerebrospinal fluid samples) were tested with the Meridian Premier Coccidioides enzyme immunoassay (MPC-EIA), and the results were compared with those of conventional serologic tests detecting complement-fixing (CF) and tube precipitin (TP) antibodies. Of the 73 positive specimens by CF, 72 were positive by MPC-EIA (72 IgG positive, 48 IgM positive) and 1 was indeterminate. Of the 132 specimens negative by CF, 126 were negative by MPC-EIA, 3 were IgM positive only, and 3 were indeterminate. Of the three patients with specimens positive for IgM only, CF demonstrated seroconversion within a month in one. All 21 specimens that displayed anticomplementary activity by CF were confirmed negative by agar gel immunodiffusion for CF antibodies (IDCF) and TP antibodies (IDTP); 20 of these serum samples were also negative by MPC-EIA, and 1 was indeterminate. After exclusion of the 25 specimens with anticomplementary or indeterminate activity, results of MPC-EIA were in agreement with those of CF in 99% of specimens. With CF as reference, MPC-EIA had a specificity of 98%, sensitivity of 100%, and positive and negative predictive values of 96% and 100%, respectively. Correlation coefficient values from linear regressions of log-2 CF titer vs MPC-EIA IgG index values were calculated at .290 for serum samples and .931 for cerebrospinal fluid.

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Authors+Show Affiliations

Zartarian M
Department of Pathology, University of California Irvine, Medical Center, Orange 92668-4805, USA.
Peterson EM
No affiliation info available
de la Maza LM
No affiliation info available

MeSH

Antibodies, FungalCoccidioidesCoccidioidomycosisComplement Fixation TestsHumansImmunoenzyme TechniquesImmunoglobulin GImmunoglobulin MLatex Fixation Tests

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

9024063

Citation

Zartarian, M, et al. "Detection of Antibodies to Coccidioides Immitis By Enzyme Immunoassay." American Journal of Clinical Pathology, vol. 107, no. 2, 1997, pp. 148-53.
Zartarian M, Peterson EM, de la Maza LM. Detection of antibodies to Coccidioides immitis by enzyme immunoassay. Am J Clin Pathol. 1997;107(2):148-53.
Zartarian, M., Peterson, E. M., & de la Maza, L. M. (1997). Detection of antibodies to Coccidioides immitis by enzyme immunoassay. American Journal of Clinical Pathology, 107(2), 148-53.
Zartarian M, Peterson EM, de la Maza LM. Detection of Antibodies to Coccidioides Immitis By Enzyme Immunoassay. Am J Clin Pathol. 1997;107(2):148-53. PubMed PMID: 9024063.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of antibodies to Coccidioides immitis by enzyme immunoassay. AU - Zartarian,M, AU - Peterson,E M, AU - de la Maza,L M, PY - 1997/2/1/pubmed PY - 1997/2/1/medline PY - 1997/2/1/entrez SP - 148 EP - 53 JF - American journal of clinical pathology JO - Am J Clin Pathol VL - 107 IS - 2 N2 - Two hundred twenty-six specimens (160 serum samples, 66 cerebrospinal fluid samples) were tested with the Meridian Premier Coccidioides enzyme immunoassay (MPC-EIA), and the results were compared with those of conventional serologic tests detecting complement-fixing (CF) and tube precipitin (TP) antibodies. Of the 73 positive specimens by CF, 72 were positive by MPC-EIA (72 IgG positive, 48 IgM positive) and 1 was indeterminate. Of the 132 specimens negative by CF, 126 were negative by MPC-EIA, 3 were IgM positive only, and 3 were indeterminate. Of the three patients with specimens positive for IgM only, CF demonstrated seroconversion within a month in one. All 21 specimens that displayed anticomplementary activity by CF were confirmed negative by agar gel immunodiffusion for CF antibodies (IDCF) and TP antibodies (IDTP); 20 of these serum samples were also negative by MPC-EIA, and 1 was indeterminate. After exclusion of the 25 specimens with anticomplementary or indeterminate activity, results of MPC-EIA were in agreement with those of CF in 99% of specimens. With CF as reference, MPC-EIA had a specificity of 98%, sensitivity of 100%, and positive and negative predictive values of 96% and 100%, respectively. Correlation coefficient values from linear regressions of log-2 CF titer vs MPC-EIA IgG index values were calculated at .290 for serum samples and .931 for cerebrospinal fluid. SN - 0002-9173 UR - https://www.unboundmedicine.com/medline/citation/9024063/Detection_of_antibodies_to_Coccidioides_immitis_by_enzyme_immunoassay_ L2 - https://academic.oup.com/ajcp/article-lookup/doi/10.1093/ajcp/107.2.148 DB - PRIME DP - Unbound Medicine ER -