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An indirect immunofluorescent test for detection of rabies virus antibodies in foxes.
J Wildl Dis. 1997 Jan; 33(1):143-5.JW

Abstract

The blood-containing fluids in the thoracic cavity or blood from the heart from 177 red foxes (Vulpes vulpes) in Slovenia were evaluated for rabies antibodies by rapid fluorescent focus inhibition test (RFFIT) and an adapted indirect immunofluorescent test (IIF) in 1994. We evaluated the usefulness of anti-dog fluorescein-isothiocyanate (FITC) conjugate instead of anti-fox FITC conjugate in detection of antibodies against rabies virus in fox sera. In the RFFIT test, 92 (52%) of the fox samples were positive and 70 (40%) samples were negative for rabies antibodies; 15 (8.5%) samples were not suitable for examination in this test. In the IIF test, 98 (55%) fox samples were positive and 79 (45%) sera were negative. The IIF test was suitable for the rapid detection of antibodies against rabies virus in foxes, as often required for vaccine efficacy trials.

Links

Publisher Full Text
jwildlifedis.org
doi.org
meridian.allenpress.com

Authors+Show Affiliations

Hostnik P
Veterinary Faculty, Ljubijana, Slovenia.
Grom J
No affiliation info available

MeSH

Administration, OralAnimalsAntibodies, ViralFluorescent Antibody Technique, IndirectFoxesRabiesRabies VaccinesRabies virusSloveniaVaccination

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9027703

Citation

Hostnik, P, and J Grom. "An Indirect Immunofluorescent Test for Detection of Rabies Virus Antibodies in Foxes." Journal of Wildlife Diseases, vol. 33, no. 1, 1997, pp. 143-5.
Hostnik P, Grom J. An indirect immunofluorescent test for detection of rabies virus antibodies in foxes. J Wildl Dis. 1997;33(1):143-5.
Hostnik, P., & Grom, J. (1997). An indirect immunofluorescent test for detection of rabies virus antibodies in foxes. Journal of Wildlife Diseases, 33(1), 143-5.
Hostnik P, Grom J. An Indirect Immunofluorescent Test for Detection of Rabies Virus Antibodies in Foxes. J Wildl Dis. 1997;33(1):143-5. PubMed PMID: 9027703.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An indirect immunofluorescent test for detection of rabies virus antibodies in foxes. AU - Hostnik,P, AU - Grom,J, PY - 1997/1/1/pubmed PY - 1997/1/1/medline PY - 1997/1/1/entrez SP - 143 EP - 5 JF - Journal of wildlife diseases JO - J Wildl Dis VL - 33 IS - 1 N2 - The blood-containing fluids in the thoracic cavity or blood from the heart from 177 red foxes (Vulpes vulpes) in Slovenia were evaluated for rabies antibodies by rapid fluorescent focus inhibition test (RFFIT) and an adapted indirect immunofluorescent test (IIF) in 1994. We evaluated the usefulness of anti-dog fluorescein-isothiocyanate (FITC) conjugate instead of anti-fox FITC conjugate in detection of antibodies against rabies virus in fox sera. In the RFFIT test, 92 (52%) of the fox samples were positive and 70 (40%) samples were negative for rabies antibodies; 15 (8.5%) samples were not suitable for examination in this test. In the IIF test, 98 (55%) fox samples were positive and 79 (45%) sera were negative. The IIF test was suitable for the rapid detection of antibodies against rabies virus in foxes, as often required for vaccine efficacy trials. SN - 0090-3558 UR - https://www.unboundmedicine.com/medline/citation/9027703/An_indirect_immunofluorescent_test_for_detection_of_rabies_virus_antibodies_in_foxes_ L2 - http://www.jwildlifedis.org/doi/10.7589/0090-3558-33.1.143?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -