Tags

Type your tag names separated by a space and hit enter

Utilization of dilute Russell's viper venom time to detect autoantibodies against beta 2-glycoprotein I which express anticoagulant activity in the presence but not in the absence of exogenous phospholipids.
Thromb Haemost. 1997 Jan; 77(1):123-6.TH

Abstract

Lupus anticoagulant (LA) is a general term to define immunoglobulins interfering with phospholipid-dependent coagulation tests. It is now clear that the phospholipid-dependence of some LA is related to the presence of the phospholipid-binding plasma protein beta 2-glycoprotein I (beta 2-GPI) and that autoantibodies to beta 2-GPI might represent a specific category of LA. To verify this hypothesis we have purified IgG autoantibodies to beta 2-GPI from plasma of 6 patients with antiphospholipid antibody syndrome, by means of agarose-immobilized human beta 2-GPI. All 6 preparations tested positive in anti-beta 2-GPI IgG antibody ELISA and showed a marked LA activity by prolonging dilute Russell Viper Venom Time (dRVVT) from a minimum of 5.3 s in patient # 1 to a maximum of 41.1 s in patient # 3. These IgG preparations behaved as typical LA, with this activity tending to disappear in the presence of increasing phospholipid (PL) concentrations. Moreover, the LA activity of the IgG preparations was not detectable in the absence of PL, in which case the ratio between dRVVT obtained in the presence and absence of IgG autoantibodies to beta 2-GPI was close to 1. This pattern was confirmed by using plasma from patients with antiphospholipid antibody syndrome testing positive for anti-beta 2-GPI IgG antibodies. These findings suggest that dRVVT performed both in the presence and absence of PL might constitute a sensitive screening test to detect specific antibodies with LA activity.

Authors+Show Affiliations

Department of Clinical and Experimental Medicine, University of Padova, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9031461

Citation

Pengo, V, et al. "Utilization of Dilute Russell's Viper Venom Time to Detect Autoantibodies Against Beta 2-glycoprotein I Which Express Anticoagulant Activity in the Presence but Not in the Absence of Exogenous Phospholipids." Thrombosis and Haemostasis, vol. 77, no. 1, 1997, pp. 123-6.
Pengo V, Balestrieri G, Tincani A, et al. Utilization of dilute Russell's viper venom time to detect autoantibodies against beta 2-glycoprotein I which express anticoagulant activity in the presence but not in the absence of exogenous phospholipids. Thromb Haemost. 1997;77(1):123-6.
Pengo, V., Balestrieri, G., Tincani, A., Spatola, L., Biasiolo, A., & Brocco, T. (1997). Utilization of dilute Russell's viper venom time to detect autoantibodies against beta 2-glycoprotein I which express anticoagulant activity in the presence but not in the absence of exogenous phospholipids. Thrombosis and Haemostasis, 77(1), 123-6.
Pengo V, et al. Utilization of Dilute Russell's Viper Venom Time to Detect Autoantibodies Against Beta 2-glycoprotein I Which Express Anticoagulant Activity in the Presence but Not in the Absence of Exogenous Phospholipids. Thromb Haemost. 1997;77(1):123-6. PubMed PMID: 9031461.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Utilization of dilute Russell's viper venom time to detect autoantibodies against beta 2-glycoprotein I which express anticoagulant activity in the presence but not in the absence of exogenous phospholipids. AU - Pengo,V, AU - Balestrieri,G, AU - Tincani,A, AU - Spatola,L, AU - Biasiolo,A, AU - Brocco,T, PY - 1997/1/1/pubmed PY - 1997/1/1/medline PY - 1997/1/1/entrez SP - 123 EP - 6 JF - Thrombosis and haemostasis JO - Thromb Haemost VL - 77 IS - 1 N2 - Lupus anticoagulant (LA) is a general term to define immunoglobulins interfering with phospholipid-dependent coagulation tests. It is now clear that the phospholipid-dependence of some LA is related to the presence of the phospholipid-binding plasma protein beta 2-glycoprotein I (beta 2-GPI) and that autoantibodies to beta 2-GPI might represent a specific category of LA. To verify this hypothesis we have purified IgG autoantibodies to beta 2-GPI from plasma of 6 patients with antiphospholipid antibody syndrome, by means of agarose-immobilized human beta 2-GPI. All 6 preparations tested positive in anti-beta 2-GPI IgG antibody ELISA and showed a marked LA activity by prolonging dilute Russell Viper Venom Time (dRVVT) from a minimum of 5.3 s in patient # 1 to a maximum of 41.1 s in patient # 3. These IgG preparations behaved as typical LA, with this activity tending to disappear in the presence of increasing phospholipid (PL) concentrations. Moreover, the LA activity of the IgG preparations was not detectable in the absence of PL, in which case the ratio between dRVVT obtained in the presence and absence of IgG autoantibodies to beta 2-GPI was close to 1. This pattern was confirmed by using plasma from patients with antiphospholipid antibody syndrome testing positive for anti-beta 2-GPI IgG antibodies. These findings suggest that dRVVT performed both in the presence and absence of PL might constitute a sensitive screening test to detect specific antibodies with LA activity. SN - 0340-6245 UR - https://www.unboundmedicine.com/medline/citation/9031461/Utilization_of_dilute_Russell's_viper_venom_time_to_detect_autoantibodies_against_beta_2_glycoprotein_I_which_express_anticoagulant_activity_in_the_presence_but_not_in_the_absence_of_exogenous_phospholipids_ L2 - https://antibodies.cancer.gov/detail/CPTC-GPI-1 DB - PRIME DP - Unbound Medicine ER -