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Rapid loading of large sample volumes, analyte cleanup, and modified moving boundary transient isotachophoresis conditions for membrane preconcentration-capillary electrophoresis in small diameter capillaries.
Electrophoresis. 1996 Dec; 17(12):1801-7.E

Abstract

Using a removable membrane preconcentration (mPC) cartridge, large sample volumes can be loaded prior to final assembly of the mPC capillary electrophoresis (CE) capillary. For narrow-bore (< or = 25 microns ID) uncoated mPC-CE capillaries, applied to peptide analysis, efficient moving boundary transient isotachphoresis (tITP) conditions at the onset of electrophoresis are described. The enhancement of mPC-CE-mass spectrometry (MS) technology afforded by rapid sample loading and modified moving boundary tITP conditions are demonstrated by analysis of major histocompatibility complex (MHC) class I peptides that were derived from a Kb precipitation of mouse EL-4 cells. Furthermore, we demonstrate the structural characterization of these immunologically significant molecules by mPC-CE-tandem mass spectrometry (mPC-CE-MS/MS).

Authors+Show Affiliations

Tomlinson AJ
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA.
Benson LM
No affiliation info available
Jameson S
No affiliation info available
Naylor S
No affiliation info available

MeSH

Amino Acid SequenceAnimalsCell LineChromatography, High Pressure LiquidElectrophoresis, CapillaryHistocompatibility Antigens Class IMass SpectrometryMicePeptides

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9034760

Citation

Tomlinson, A J., et al. "Rapid Loading of Large Sample Volumes, Analyte Cleanup, and Modified Moving Boundary Transient Isotachophoresis Conditions for Membrane Preconcentration-capillary Electrophoresis in Small Diameter Capillaries." Electrophoresis, vol. 17, no. 12, 1996, pp. 1801-7.
Tomlinson AJ, Benson LM, Jameson S, et al. Rapid loading of large sample volumes, analyte cleanup, and modified moving boundary transient isotachophoresis conditions for membrane preconcentration-capillary electrophoresis in small diameter capillaries. Electrophoresis. 1996;17(12):1801-7.
Tomlinson, A. J., Benson, L. M., Jameson, S., & Naylor, S. (1996). Rapid loading of large sample volumes, analyte cleanup, and modified moving boundary transient isotachophoresis conditions for membrane preconcentration-capillary electrophoresis in small diameter capillaries. Electrophoresis, 17(12), 1801-7.
Tomlinson AJ, et al. Rapid Loading of Large Sample Volumes, Analyte Cleanup, and Modified Moving Boundary Transient Isotachophoresis Conditions for Membrane Preconcentration-capillary Electrophoresis in Small Diameter Capillaries. Electrophoresis. 1996;17(12):1801-7. PubMed PMID: 9034760.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid loading of large sample volumes, analyte cleanup, and modified moving boundary transient isotachophoresis conditions for membrane preconcentration-capillary electrophoresis in small diameter capillaries. AU - Tomlinson,A J, AU - Benson,L M, AU - Jameson,S, AU - Naylor,S, PY - 1996/12/1/pubmed PY - 1996/12/1/medline PY - 1996/12/1/entrez SP - 1801 EP - 7 JF - Electrophoresis JO - Electrophoresis VL - 17 IS - 12 N2 - Using a removable membrane preconcentration (mPC) cartridge, large sample volumes can be loaded prior to final assembly of the mPC capillary electrophoresis (CE) capillary. For narrow-bore (< or = 25 microns ID) uncoated mPC-CE capillaries, applied to peptide analysis, efficient moving boundary transient isotachphoresis (tITP) conditions at the onset of electrophoresis are described. The enhancement of mPC-CE-mass spectrometry (MS) technology afforded by rapid sample loading and modified moving boundary tITP conditions are demonstrated by analysis of major histocompatibility complex (MHC) class I peptides that were derived from a Kb precipitation of mouse EL-4 cells. Furthermore, we demonstrate the structural characterization of these immunologically significant molecules by mPC-CE-tandem mass spectrometry (mPC-CE-MS/MS). SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/9034760/Rapid_loading_of_large_sample_volumes_analyte_cleanup_and_modified_moving_boundary_transient_isotachophoresis_conditions_for_membrane_preconcentration_capillary_electrophoresis_in_small_diameter_capillaries_ L2 - https://doi.org/10.1002/elps.1150171203 DB - PRIME DP - Unbound Medicine ER -