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Linearity evaluation in absorbance detection: the use of light-emitting diodes for on-capillary detection in capillary electrophoresis.
Electrophoresis. 1996 Dec; 17(12):1898-905.E

Abstract

A model which takes into account both stray light and polychromatic light was used to predict and evaluate linearity in on-capillary detection in capillary electrophoresis (CE). According to the model the stray light is the major factor which determines linearity under typical CE operating conditions. By calculating theoretical absorbance versus concentration plots, the influence of different levels of stray light and polychromatic light on linearity is demonstrated. Experimentally, six light-emitting diodes (LEDs) in the range from 563 to 654 nm were examined as light sources for on-capillary detection in CE. Fitting theoretical curves to measured linearity plots enabled determination of the values of both effective path length and stray light for a particular detection system. The detector linearity for the four LEDs was compared to mercury and tungsten lamps used with interference filters. For potassium permanganate as the test compound, the linear range for a 563 nm LED was two times greater than that for a mercury lamp operated at 546 nm. The relatively poor linearity of the mercury lamp detector is explained by its high level of stray light. The noise of the LED563-based detector was the same as for the mercury lamp, whereas the other LEDs of higher light intensity gave approximately half the noise of the mercury lamp. The lowest noise level of 3 x 10(-5) AU was obtained for the LED at 554 nm (determined at a detector time constant of 0.1 s).

Authors+Show Affiliations

Macka M
Department of Chemistry, University of Tasmania, Hobart, Australia.
Andersson P
No affiliation info available
Haddad PR
No affiliation info available

MeSH

Electrophoresis, CapillaryLightMathematicsModels, ChemicalSpectrophotometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9034772

Citation

Macka, M, et al. "Linearity Evaluation in Absorbance Detection: the Use of Light-emitting Diodes for On-capillary Detection in Capillary Electrophoresis." Electrophoresis, vol. 17, no. 12, 1996, pp. 1898-905.
Macka M, Andersson P, Haddad PR. Linearity evaluation in absorbance detection: the use of light-emitting diodes for on-capillary detection in capillary electrophoresis. Electrophoresis. 1996;17(12):1898-905.
Macka, M., Andersson, P., & Haddad, P. R. (1996). Linearity evaluation in absorbance detection: the use of light-emitting diodes for on-capillary detection in capillary electrophoresis. Electrophoresis, 17(12), 1898-905.
Macka M, Andersson P, Haddad PR. Linearity Evaluation in Absorbance Detection: the Use of Light-emitting Diodes for On-capillary Detection in Capillary Electrophoresis. Electrophoresis. 1996;17(12):1898-905. PubMed PMID: 9034772.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Linearity evaluation in absorbance detection: the use of light-emitting diodes for on-capillary detection in capillary electrophoresis. AU - Macka,M, AU - Andersson,P, AU - Haddad,P R, PY - 1996/12/1/pubmed PY - 1996/12/1/medline PY - 1996/12/1/entrez SP - 1898 EP - 905 JF - Electrophoresis JO - Electrophoresis VL - 17 IS - 12 N2 - A model which takes into account both stray light and polychromatic light was used to predict and evaluate linearity in on-capillary detection in capillary electrophoresis (CE). According to the model the stray light is the major factor which determines linearity under typical CE operating conditions. By calculating theoretical absorbance versus concentration plots, the influence of different levels of stray light and polychromatic light on linearity is demonstrated. Experimentally, six light-emitting diodes (LEDs) in the range from 563 to 654 nm were examined as light sources for on-capillary detection in CE. Fitting theoretical curves to measured linearity plots enabled determination of the values of both effective path length and stray light for a particular detection system. The detector linearity for the four LEDs was compared to mercury and tungsten lamps used with interference filters. For potassium permanganate as the test compound, the linear range for a 563 nm LED was two times greater than that for a mercury lamp operated at 546 nm. The relatively poor linearity of the mercury lamp detector is explained by its high level of stray light. The noise of the LED563-based detector was the same as for the mercury lamp, whereas the other LEDs of higher light intensity gave approximately half the noise of the mercury lamp. The lowest noise level of 3 x 10(-5) AU was obtained for the LED at 554 nm (determined at a detector time constant of 0.1 s). SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/9034772/Linearity_evaluation_in_absorbance_detection:_the_use_of_light_emitting_diodes_for_on_capillary_detection_in_capillary_electrophoresis_ L2 - https://doi.org/10.1002/elps.1150171215 DB - PRIME DP - Unbound Medicine ER -