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Rational use of polymerase chain reaction--based detection of viral genomes in patients with serologic markers of hepatitis B or C virus infection.
Wien Klin Wochenschr. 1997 Jan 17; 109(1):20-4.WK

Abstract

We studied the value of additional diagnostic information obtained by detection of hepatitis B virus (HBV) DNA or hepatitis C virus (HCV) RNA using the qualitative polymerase chain reaction (PCR) in patients with serologic markers of hepatitis B or hepatitis C virus infection. In HBV infection, all HBsAg+HBeAg+ patients and all HBsAg+HBeAg- patients with alanine aminotransferase (ALT) levels > 100 U/L were positive for HBV-DNA by PCR, whereas in HBsAg+HBeAg- patients with ALT < 100 U/L 58% and in HBsAg+HBeAg- patients with normal aminotransferase 45% were found to be positive. In HBsAg+ patients no further clinically useful information can be obtained by PCR as the presence of HBsAg proves infection. However in three of 42 (7%) patients with markers of past HBV infection (antiHBs and/or antiHBc+) HBV-DNA was detected in the serum. Similarly, in some patients with acute hepatitis B HBV-DNA was demonstrable up to four months after the disappearance of HBsAg from serum, pointing to persistence of viremia despite the loss of serological markers of ongoing HBV infection. Demonstrating ongoing HBV infection in patients with serological markers of past infection is valuable additional information in only selected patients. In HCV infection, 10% of anti-HCV+ patients with increased ALT levels had a negative serum HCV-RNA. However, in 20% of those patients HCV-RNA was demonstrated in a serum sample collected later during follow-up, indicating that a single negative HCV-RNA determination cannot be taken as evidence for the resolution of infection.

Authors+Show Affiliations

Universitätsklinik für Innere Medizin IV, Wien, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9037744

Citation

Pfeffel, F, et al. "Rational Use of Polymerase Chain Reaction--based Detection of Viral Genomes in Patients With Serologic Markers of Hepatitis B or C Virus Infection." Wiener Klinische Wochenschrift, vol. 109, no. 1, 1997, pp. 20-4.
Pfeffel F, Oesterreicher C, Penner E, et al. Rational use of polymerase chain reaction--based detection of viral genomes in patients with serologic markers of hepatitis B or C virus infection. Wien Klin Wochenschr. 1997;109(1):20-4.
Pfeffel, F., Oesterreicher, C., Penner, E., Ferenci, P., Sillaber, C., Fiedler, R., & Müller, C. (1997). Rational use of polymerase chain reaction--based detection of viral genomes in patients with serologic markers of hepatitis B or C virus infection. Wiener Klinische Wochenschrift, 109(1), 20-4.
Pfeffel F, et al. Rational Use of Polymerase Chain Reaction--based Detection of Viral Genomes in Patients With Serologic Markers of Hepatitis B or C Virus Infection. Wien Klin Wochenschr. 1997 Jan 17;109(1):20-4. PubMed PMID: 9037744.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rational use of polymerase chain reaction--based detection of viral genomes in patients with serologic markers of hepatitis B or C virus infection. AU - Pfeffel,F, AU - Oesterreicher,C, AU - Penner,E, AU - Ferenci,P, AU - Sillaber,C, AU - Fiedler,R, AU - Müller,C, PY - 1997/1/17/pubmed PY - 1997/1/17/medline PY - 1997/1/17/entrez SP - 20 EP - 4 JF - Wiener klinische Wochenschrift JO - Wien. Klin. Wochenschr. VL - 109 IS - 1 N2 - We studied the value of additional diagnostic information obtained by detection of hepatitis B virus (HBV) DNA or hepatitis C virus (HCV) RNA using the qualitative polymerase chain reaction (PCR) in patients with serologic markers of hepatitis B or hepatitis C virus infection. In HBV infection, all HBsAg+HBeAg+ patients and all HBsAg+HBeAg- patients with alanine aminotransferase (ALT) levels > 100 U/L were positive for HBV-DNA by PCR, whereas in HBsAg+HBeAg- patients with ALT < 100 U/L 58% and in HBsAg+HBeAg- patients with normal aminotransferase 45% were found to be positive. In HBsAg+ patients no further clinically useful information can be obtained by PCR as the presence of HBsAg proves infection. However in three of 42 (7%) patients with markers of past HBV infection (antiHBs and/or antiHBc+) HBV-DNA was detected in the serum. Similarly, in some patients with acute hepatitis B HBV-DNA was demonstrable up to four months after the disappearance of HBsAg from serum, pointing to persistence of viremia despite the loss of serological markers of ongoing HBV infection. Demonstrating ongoing HBV infection in patients with serological markers of past infection is valuable additional information in only selected patients. In HCV infection, 10% of anti-HCV+ patients with increased ALT levels had a negative serum HCV-RNA. However, in 20% of those patients HCV-RNA was demonstrated in a serum sample collected later during follow-up, indicating that a single negative HCV-RNA determination cannot be taken as evidence for the resolution of infection. SN - 0043-5325 UR - https://www.unboundmedicine.com/medline/citation/9037744/Rational_use_of_polymerase_chain_reaction__based_detection_of_viral_genomes_in_patients_with_serologic_markers_of_hepatitis_B_or_C_virus_infection_ L2 - http://www.diseaseinfosearch.org/result/3332 DB - PRIME DP - Unbound Medicine ER -