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Solid-phase microextraction for the analysis of human breath.
Anal Chem. 1997 Feb 15; 69(4):587-96.AC

Abstract

Solid-phase microextraction (SPME) has been applied to the quantitative determination of ethanol, acetone, and isoprene in human breath. The method involves extraction and preconcentration with a fused silica fiber coated with a polymeric stationary phase, desorption at 200 degrees C, and assay by gas chromatography/mass spectrometry. Three different fiber coatings have been evaluated with regard to sensitivity, linear range, precision, and detection limits. Typical RSD values in the range 2%-6% could be obtained, depending on the fiber coating and the compound investigated. The calibration curves for the compounds are reproducible and linear over the concentration ranges found in human breath samples. The method is capable of detecting concentrations of acetone and isoprene reported for healthy subjects. The influence of temperature and humidity on the extraction process has been studied in detail. A linear relationship between log K versus 1/T allows the calibration of the method for any given temperature. The device is portable, economical, and easy to use in patient sampling.

Authors+Show Affiliations

Guelph-Waterloo Center for Graduate Work in Chemistry, Department of Chemistry, University of Waterloo, Ontario, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9043197

Citation

Grote, C, and J Pawliszyn. "Solid-phase Microextraction for the Analysis of Human Breath." Analytical Chemistry, vol. 69, no. 4, 1997, pp. 587-96.
Grote C, Pawliszyn J. Solid-phase microextraction for the analysis of human breath. Anal Chem. 1997;69(4):587-96.
Grote, C., & Pawliszyn, J. (1997). Solid-phase microextraction for the analysis of human breath. Analytical Chemistry, 69(4), 587-96.
Grote C, Pawliszyn J. Solid-phase Microextraction for the Analysis of Human Breath. Anal Chem. 1997 Feb 15;69(4):587-96. PubMed PMID: 9043197.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Solid-phase microextraction for the analysis of human breath. AU - Grote,C, AU - Pawliszyn,J, PY - 1997/2/15/pubmed PY - 1997/2/15/medline PY - 1997/2/15/entrez SP - 587 EP - 96 JF - Analytical chemistry JO - Anal Chem VL - 69 IS - 4 N2 - Solid-phase microextraction (SPME) has been applied to the quantitative determination of ethanol, acetone, and isoprene in human breath. The method involves extraction and preconcentration with a fused silica fiber coated with a polymeric stationary phase, desorption at 200 degrees C, and assay by gas chromatography/mass spectrometry. Three different fiber coatings have been evaluated with regard to sensitivity, linear range, precision, and detection limits. Typical RSD values in the range 2%-6% could be obtained, depending on the fiber coating and the compound investigated. The calibration curves for the compounds are reproducible and linear over the concentration ranges found in human breath samples. The method is capable of detecting concentrations of acetone and isoprene reported for healthy subjects. The influence of temperature and humidity on the extraction process has been studied in detail. A linear relationship between log K versus 1/T allows the calibration of the method for any given temperature. The device is portable, economical, and easy to use in patient sampling. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/9043197/Solid_phase_microextraction_for_the_analysis_of_human_breath_ L2 - https://doi.org/10.1021/ac960749l DB - PRIME DP - Unbound Medicine ER -