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[Usefulness of an ELISA for the diagnosis of Streptococcus pneumoniae in patients with pneumonia].
Enferm Infecc Microbiol Clin. 1996 Apr; 14(4):255-7.EI

Abstract

BACKGROUND

To evaluate the etiologic role of Streptococcus pneumoniae in a series of patients with community-acquired pneumonia.

METHODS

By an direct ELISA the presence of Streptococcus pneumoniae capsular polysaccharide antigen in serum and urine from 125 patients with community pneumonia was evaluated. Horseradish peroxidase-labeled polyvalent antiserum (Omniserum) was used. Working dilutions of reagents were determined by checkerboarding. Positive control antigen consisted of Pneumovax 23 and negative control consisted of serum and urine samples from healthy subjects.

RESULTS

Antigen detection was positive in 8 of 124 (6.45%; CI 95%: 2.80-12.30%) serum samples and in 15 of 115 (13.04%; CI 95%; 7.51-20.68%) urine samples concentrated 25-fold. Pneumococcal pneumonia was diagnosed by ELISA in 17 cases (13.60%; CI 95%: 8.15-20.95%), of which one had positive blood culture. The sensitivity of the ELISA in 24 patients with definite pneumococcal pneumonia diagnosed in this series was 70.83%.

CONCLUSION

These results suggest that the ELISA is a useful method in rapid diagnosis of Streptococcus pneumoniae in patients with pneumonia.

Authors+Show Affiliations

Mata M
Departamento de Microbiología, Facultad de Biología, Universidad de Santiago de Compostela.
Sesma P
No affiliation info available
Bernárdez I
No affiliation info available

MeSH

AdolescentAdultAgedAged, 80 and overAntibodies, BacterialAntigens, BacterialChildCommunity-Acquired InfectionsEnzyme-Linked Immunosorbent AssayFemaleHumansMaleMiddle AgedPneumonia, PneumococcalPolysaccharides, BacterialSensitivity and SpecificityStreptococcus pneumoniae

Pub Type(s)

English Abstract
Journal Article

Language

spa

PubMed ID

9044642

Citation

Mata, M, et al. "[Usefulness of an ELISA for the Diagnosis of Streptococcus Pneumoniae in Patients With Pneumonia]." Enfermedades Infecciosas Y Microbiologia Clinica, vol. 14, no. 4, 1996, pp. 255-7.
Mata M, Sesma P, Bernárdez I. [Usefulness of an ELISA for the diagnosis of Streptococcus pneumoniae in patients with pneumonia]. Enferm Infecc Microbiol Clin. 1996;14(4):255-7.
Mata, M., Sesma, P., & Bernárdez, I. (1996). [Usefulness of an ELISA for the diagnosis of Streptococcus pneumoniae in patients with pneumonia]. Enfermedades Infecciosas Y Microbiologia Clinica, 14(4), 255-7.
Mata M, Sesma P, Bernárdez I. [Usefulness of an ELISA for the Diagnosis of Streptococcus Pneumoniae in Patients With Pneumonia]. Enferm Infecc Microbiol Clin. 1996;14(4):255-7. PubMed PMID: 9044642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Usefulness of an ELISA for the diagnosis of Streptococcus pneumoniae in patients with pneumonia]. AU - Mata,M, AU - Sesma,P, AU - Bernárdez,I, PY - 1996/4/1/pubmed PY - 1996/4/1/medline PY - 1996/4/1/entrez SP - 255 EP - 7 JF - Enfermedades infecciosas y microbiologia clinica JO - Enferm Infecc Microbiol Clin VL - 14 IS - 4 N2 - BACKGROUND: To evaluate the etiologic role of Streptococcus pneumoniae in a series of patients with community-acquired pneumonia. METHODS: By an direct ELISA the presence of Streptococcus pneumoniae capsular polysaccharide antigen in serum and urine from 125 patients with community pneumonia was evaluated. Horseradish peroxidase-labeled polyvalent antiserum (Omniserum) was used. Working dilutions of reagents were determined by checkerboarding. Positive control antigen consisted of Pneumovax 23 and negative control consisted of serum and urine samples from healthy subjects. RESULTS: Antigen detection was positive in 8 of 124 (6.45%; CI 95%: 2.80-12.30%) serum samples and in 15 of 115 (13.04%; CI 95%; 7.51-20.68%) urine samples concentrated 25-fold. Pneumococcal pneumonia was diagnosed by ELISA in 17 cases (13.60%; CI 95%: 8.15-20.95%), of which one had positive blood culture. The sensitivity of the ELISA in 24 patients with definite pneumococcal pneumonia diagnosed in this series was 70.83%. CONCLUSION: These results suggest that the ELISA is a useful method in rapid diagnosis of Streptococcus pneumoniae in patients with pneumonia. SN - 0213-005X UR - https://www.unboundmedicine.com/medline/citation/9044642/[Usefulness_of_an_ELISA_for_the_diagnosis_of_Streptococcus_pneumoniae_in_patients_with_pneumonia]_ DB - PRIME DP - Unbound Medicine ER -