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Infection and replication of human cytomegalovirus in bone marrow stromal cells: effects on the production of IL-6, MIP-1alpha, and TGF-beta1.
Bone Marrow Transplant. 1997 Mar; 19(5):471-80.BM

Abstract

We have investigated the mechanisms by which hematopoiesis is suppressed in patients suffering from human cytomegalovirus (HCMV) infections. Mixed populations of human bone marrow stromal and hematopoietic progenitor cells were inoculated with the Towne strain of HCMV to determine whether these populations could be infected and support HCMV replication. We found that the Towne strain of HCMV was capable of infecting and replicating in a mixed population of bone marrow stromal cells. We observed no significant alterations in bone marrow stromal cell proliferation or the production of IL-6, GM-CSF, soluble c-kit ligand and TNF-alpha following HCMV replication in either stimulated lipopolysaccharide (LPS) or unstimulated conditions. In samples of culture supernatants from LPS-stimulated HCMV-infected stromal cells, significant elevations in MIP-1alpha were observed. TGF-beta1 levels on the other hand exhibited two patterns following HCMV exposure; either TGF-beta1 levels decreased regardless of LPS stimulation or there was no effect. In addition, we observed that exposure to the Towne strain of HCMV resulted in significant inhibition of both granulocytic and erythrocytic colony formation in methylcellulose progenitor assays. Thus, both the direct effect of HCMV on hematopoietic progenitors as well as altered cytokine production by bone marrow stromal cells (including MIP-1alpha and TGF-beta1, but not IL-6) could contribute to hematopoietic failure during HCMV infection.

Authors+Show Affiliations

Taichman RS
Department of Periodontics/Prevention/Geriatrics, School of Dentistry, University of Michigan, Ann Arbor 48109-1078, USA.
Nassiri MR
No affiliation info available
Reilly MJ
No affiliation info available
Ptak RG
No affiliation info available
Emerson SG
No affiliation info available
Drach JC
No affiliation info available

MeSH

Adipose TissueBone MarrowBone Marrow CellsCells, CulturedChemokine CCL3Chemokine CCL4Connective TissueConnective Tissue CellsCulture Media, ConditionedCytomegalovirusGranulocyte-Macrophage Colony-Stimulating FactorHumansInterleukin-6Macrophage Inflammatory ProteinsStem Cell FactorTransforming Growth Factor betaTumor Necrosis Factor-alphaVirus Replication

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9052914

Citation

Taichman, R S., et al. "Infection and Replication of Human Cytomegalovirus in Bone Marrow Stromal Cells: Effects On the Production of IL-6, MIP-1alpha, and TGF-beta1." Bone Marrow Transplantation, vol. 19, no. 5, 1997, pp. 471-80.
Taichman RS, Nassiri MR, Reilly MJ, et al. Infection and replication of human cytomegalovirus in bone marrow stromal cells: effects on the production of IL-6, MIP-1alpha, and TGF-beta1. Bone Marrow Transplant. 1997;19(5):471-80.
Taichman, R. S., Nassiri, M. R., Reilly, M. J., Ptak, R. G., Emerson, S. G., & Drach, J. C. (1997). Infection and replication of human cytomegalovirus in bone marrow stromal cells: effects on the production of IL-6, MIP-1alpha, and TGF-beta1. Bone Marrow Transplantation, 19(5), 471-80.
Taichman RS, et al. Infection and Replication of Human Cytomegalovirus in Bone Marrow Stromal Cells: Effects On the Production of IL-6, MIP-1alpha, and TGF-beta1. Bone Marrow Transplant. 1997;19(5):471-80. PubMed PMID: 9052914.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Infection and replication of human cytomegalovirus in bone marrow stromal cells: effects on the production of IL-6, MIP-1alpha, and TGF-beta1. AU - Taichman,R S, AU - Nassiri,M R, AU - Reilly,M J, AU - Ptak,R G, AU - Emerson,S G, AU - Drach,J C, PY - 1997/3/1/pubmed PY - 1997/3/1/medline PY - 1997/3/1/entrez SP - 471 EP - 80 JF - Bone marrow transplantation JO - Bone Marrow Transplant VL - 19 IS - 5 N2 - We have investigated the mechanisms by which hematopoiesis is suppressed in patients suffering from human cytomegalovirus (HCMV) infections. Mixed populations of human bone marrow stromal and hematopoietic progenitor cells were inoculated with the Towne strain of HCMV to determine whether these populations could be infected and support HCMV replication. We found that the Towne strain of HCMV was capable of infecting and replicating in a mixed population of bone marrow stromal cells. We observed no significant alterations in bone marrow stromal cell proliferation or the production of IL-6, GM-CSF, soluble c-kit ligand and TNF-alpha following HCMV replication in either stimulated lipopolysaccharide (LPS) or unstimulated conditions. In samples of culture supernatants from LPS-stimulated HCMV-infected stromal cells, significant elevations in MIP-1alpha were observed. TGF-beta1 levels on the other hand exhibited two patterns following HCMV exposure; either TGF-beta1 levels decreased regardless of LPS stimulation or there was no effect. In addition, we observed that exposure to the Towne strain of HCMV resulted in significant inhibition of both granulocytic and erythrocytic colony formation in methylcellulose progenitor assays. Thus, both the direct effect of HCMV on hematopoietic progenitors as well as altered cytokine production by bone marrow stromal cells (including MIP-1alpha and TGF-beta1, but not IL-6) could contribute to hematopoietic failure during HCMV infection. SN - 0268-3369 UR - https://www.unboundmedicine.com/medline/citation/9052914/Infection_and_replication_of_human_cytomegalovirus_in_bone_marrow_stromal_cells:_effects_on_the_production_of_IL_6_MIP_1alpha_and_TGF_beta1_ L2 - https://doi.org/10.1038/sj.bmt.1700685 DB - PRIME DP - Unbound Medicine ER -