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Activity measurements of acyl-CoA oxidases in human liver.
J Inherit Metab Dis. 1995; 18 Suppl 1:125-34.JI

Abstract

Peroxisomal beta-oxidation is involved in the degradation of different fatty acids or fatty acid derivatives including eicosanoids (prostaglandins, leukotrienes, thromboxanes), dicarboxylic fatty acids, very long-chain fatty acids, pristanic acid, bile acid intermediates (di- and trihydroxycoprostanoic acids), and xenobiotics. Separate beta-oxidation systems are probably active inside peroxisomes, each acting on a distinct set of substrates, as suggested by the discovery of multiple acyl-CoA oxidases. Using specific substrates or selective conditions, we can distinguish in rat liver the action of acyl-CoA oxidases (type I and II), a pristanoyl-CoA oxidase and a trihydroxycoprostanoyl-CoA oxidase, and, in in human liver, of acyl-CoA oxidase (type I and II) and a branched-chain acyl-CoA oxidase. When incubated with suitable CoA-esters, these different oxidases can be measured in a similar fashion by following fluorimetrically the dimerization of homovanillic acid, catalysed by peroxidase in the presence of hydrogen peroxide. The optimal assay conditions and possible pitfalls in this type of coupled assay are discussed. This knowledge can be used to reveal the existence of peroxisomal disorders in which only one acyl-CoA oxidase is deficient.

Authors+Show Affiliations

Katholieke Universiteit Leuven, Camus Gasthuisberg, Belgium.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9053547

Citation

Van Veldhoven, P P.. "Activity Measurements of acyl-CoA Oxidases in Human Liver." Journal of Inherited Metabolic Disease, vol. 18 Suppl 1, 1995, pp. 125-34.
Van Veldhoven PP. Activity measurements of acyl-CoA oxidases in human liver. J Inherit Metab Dis. 1995;18 Suppl 1:125-34.
Van Veldhoven, P. P. (1995). Activity measurements of acyl-CoA oxidases in human liver. Journal of Inherited Metabolic Disease, 18 Suppl 1, 125-34.
Van Veldhoven PP. Activity Measurements of acyl-CoA Oxidases in Human Liver. J Inherit Metab Dis. 1995;18 Suppl 1:125-34. PubMed PMID: 9053547.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activity measurements of acyl-CoA oxidases in human liver. A1 - Van Veldhoven,P P, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 125 EP - 34 JF - Journal of inherited metabolic disease JO - J Inherit Metab Dis VL - 18 Suppl 1 N2 - Peroxisomal beta-oxidation is involved in the degradation of different fatty acids or fatty acid derivatives including eicosanoids (prostaglandins, leukotrienes, thromboxanes), dicarboxylic fatty acids, very long-chain fatty acids, pristanic acid, bile acid intermediates (di- and trihydroxycoprostanoic acids), and xenobiotics. Separate beta-oxidation systems are probably active inside peroxisomes, each acting on a distinct set of substrates, as suggested by the discovery of multiple acyl-CoA oxidases. Using specific substrates or selective conditions, we can distinguish in rat liver the action of acyl-CoA oxidases (type I and II), a pristanoyl-CoA oxidase and a trihydroxycoprostanoyl-CoA oxidase, and, in in human liver, of acyl-CoA oxidase (type I and II) and a branched-chain acyl-CoA oxidase. When incubated with suitable CoA-esters, these different oxidases can be measured in a similar fashion by following fluorimetrically the dimerization of homovanillic acid, catalysed by peroxidase in the presence of hydrogen peroxide. The optimal assay conditions and possible pitfalls in this type of coupled assay are discussed. This knowledge can be used to reveal the existence of peroxisomal disorders in which only one acyl-CoA oxidase is deficient. SN - 0141-8955 UR - https://www.unboundmedicine.com/medline/citation/9053547/Activity_measurements_of_acyl_CoA_oxidases_in_human_liver_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0141-8955&date=1995&volume=18&issue=&spage=125 DB - PRIME DP - Unbound Medicine ER -