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Signals through 4-1BB are costimulatory to previously activated splenic T cells and inhibit activation-induced cell death.
J Immunol. 1997 Mar 15; 158(6):2600-9.JI

Abstract

Previously, we and others showed that signals relayed through the murine T cell Ag 4-1BB enhance primary T cell responses, and that blocking the interaction of 4-1BB with its ligand results in decreased responses to polyclonal activators and to alloantigens. Because 4-1BB expression is induced following primary stimulation, we investigated the role of signaling through this molecule in the reactivation of proliferating T cells. To this end, preactivated, 4-1BB-expressing T cells were restimulated in the presence of plate-immobilized mAbs directed against 4-1BB or the prototypic costimulatory molecule CD28. In this work, we show that in the presence of either signal, T cells respond to TCR cross-linking with strong proliferative responses and cytokine production; moreover, our findings indicate that T cell proliferation partially correlates with surface 4-1BB expression. In addition, our results suggest that Ab-mediated costimulatory signals can act independently of potential accessory B7-CD28/CTLA-4 (cytotoxic T lymphocyte Ag-4) interactions. Importantly, the characteristic DNA fragmentation and apoptotic cell death observed after TCR re-engagement are inhibited comparably in the presence of either 4-1BB or CD28 signaling.

Authors+Show Affiliations

Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9058792

Citation

Hurtado, J C., et al. "Signals Through 4-1BB Are Costimulatory to Previously Activated Splenic T Cells and Inhibit Activation-induced Cell Death." Journal of Immunology (Baltimore, Md. : 1950), vol. 158, no. 6, 1997, pp. 2600-9.
Hurtado JC, Kim YJ, Kwon BS. Signals through 4-1BB are costimulatory to previously activated splenic T cells and inhibit activation-induced cell death. J Immunol. 1997;158(6):2600-9.
Hurtado, J. C., Kim, Y. J., & Kwon, B. S. (1997). Signals through 4-1BB are costimulatory to previously activated splenic T cells and inhibit activation-induced cell death. Journal of Immunology (Baltimore, Md. : 1950), 158(6), 2600-9.
Hurtado JC, Kim YJ, Kwon BS. Signals Through 4-1BB Are Costimulatory to Previously Activated Splenic T Cells and Inhibit Activation-induced Cell Death. J Immunol. 1997 Mar 15;158(6):2600-9. PubMed PMID: 9058792.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Signals through 4-1BB are costimulatory to previously activated splenic T cells and inhibit activation-induced cell death. AU - Hurtado,J C, AU - Kim,Y J, AU - Kwon,B S, PY - 1997/3/15/pubmed PY - 1997/3/15/medline PY - 1997/3/15/entrez SP - 2600 EP - 9 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 158 IS - 6 N2 - Previously, we and others showed that signals relayed through the murine T cell Ag 4-1BB enhance primary T cell responses, and that blocking the interaction of 4-1BB with its ligand results in decreased responses to polyclonal activators and to alloantigens. Because 4-1BB expression is induced following primary stimulation, we investigated the role of signaling through this molecule in the reactivation of proliferating T cells. To this end, preactivated, 4-1BB-expressing T cells were restimulated in the presence of plate-immobilized mAbs directed against 4-1BB or the prototypic costimulatory molecule CD28. In this work, we show that in the presence of either signal, T cells respond to TCR cross-linking with strong proliferative responses and cytokine production; moreover, our findings indicate that T cell proliferation partially correlates with surface 4-1BB expression. In addition, our results suggest that Ab-mediated costimulatory signals can act independently of potential accessory B7-CD28/CTLA-4 (cytotoxic T lymphocyte Ag-4) interactions. Importantly, the characteristic DNA fragmentation and apoptotic cell death observed after TCR re-engagement are inhibited comparably in the presence of either 4-1BB or CD28 signaling. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/9058792/Signals_through_4_1BB_are_costimulatory_to_previously_activated_splenic_T_cells_and_inhibit_activation_induced_cell_death_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=9058792 DB - PRIME DP - Unbound Medicine ER -