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Evaluation of an enzyme-linked immunosorbent assay in Thai scrub typhus patients.
Am J Trop Med Hyg. 1997 Jan; 56(1):38-43.AJ

Abstract

We report the development of an improved enzyme-linked immunosorbent assay (ELISA) to detect Orientia (formerly Rickettsia) tsutsugamushi antibody in human sera. Results were compared with a standard test, the indirect immunoperoxidase assay (IIP). Control serum samples were collected from 96 American soldiers and 198 Royal Thai Army soldiers with no recent history of clinical illness. Sera were examined from 79 febrile, Thai scrub typhus patients presenting at Chiang Rai (76) and Bangkraui Nontaburi (3) Provincial hospitals (cases confirmed by elevated IIP IgG levels > or = 1:1,600, IgM levels > or = 1:400, or presence of an eschar). The mean + 2 SD, used for the upper limit of normal reactions in the IgG ELISA, was 0.10 for U.S. soldiers and 0.42 for Thai soldiers. Using the 0.10 cutoff value, 29% of the asymptomatic Thai soldiers would be designated as antibody positive. Variability of IgG ELISA values was greater in the Thai soldier group than in American soldiers, possibly reflecting previous exposure to O. tsutsugamushi. In the Thai patients, there was a significant correlation between IIP titers and single serum dilution (1:100) ELISA values (IgG, r = 0.75, n = 104; P < 0.0005; IgM, r = 0.70, n = 75; P < 0.0005) and between IIP titers and ELISA titers (IgG, r = 0.87, n = 103; P < 0.0005; IgM, r = 0.76, n = 75; P < 0.0005). The single serum dilution ELISA was as effective as the titration in determining presence of specific antibodies. The O. tsutsugamushi ELISA is a rapid and objective test amenable to accurately testing the large numbers of sera often obtained in seroepidemiologic investigations.

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Authors+Show Affiliations

Suwanabun N
Department of Entomology, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Chouriyagune C
No affiliation info available
Eamsila C
No affiliation info available
Watcharapichat P
No affiliation info available
Dasch GA
No affiliation info available
Howard RS
No affiliation info available
Kelly DJ
No affiliation info available

MeSH

AnimalsAntibodies, BacterialAntigens, BacterialCell LineEnzyme-Linked Immunosorbent AssayEvaluation Studies as TopicHumansImmunoenzyme TechniquesImmunoglobulin GMiceMilitary PersonnelOrientia tsutsugamushiROC CurveScrub TyphusSensitivity and SpecificityThailandUnited States

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

9063359

Citation

Suwanabun, N, et al. "Evaluation of an Enzyme-linked Immunosorbent Assay in Thai Scrub Typhus Patients." The American Journal of Tropical Medicine and Hygiene, vol. 56, no. 1, 1997, pp. 38-43.
Suwanabun N, Chouriyagune C, Eamsila C, et al. Evaluation of an enzyme-linked immunosorbent assay in Thai scrub typhus patients. Am J Trop Med Hyg. 1997;56(1):38-43.
Suwanabun, N., Chouriyagune, C., Eamsila, C., Watcharapichat, P., Dasch, G. A., Howard, R. S., & Kelly, D. J. (1997). Evaluation of an enzyme-linked immunosorbent assay in Thai scrub typhus patients. The American Journal of Tropical Medicine and Hygiene, 56(1), 38-43.
Suwanabun N, et al. Evaluation of an Enzyme-linked Immunosorbent Assay in Thai Scrub Typhus Patients. Am J Trop Med Hyg. 1997;56(1):38-43. PubMed PMID: 9063359.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of an enzyme-linked immunosorbent assay in Thai scrub typhus patients. AU - Suwanabun,N, AU - Chouriyagune,C, AU - Eamsila,C, AU - Watcharapichat,P, AU - Dasch,G A, AU - Howard,R S, AU - Kelly,D J, PY - 1997/1/1/pubmed PY - 1997/1/1/medline PY - 1997/1/1/entrez SP - 38 EP - 43 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 56 IS - 1 N2 - We report the development of an improved enzyme-linked immunosorbent assay (ELISA) to detect Orientia (formerly Rickettsia) tsutsugamushi antibody in human sera. Results were compared with a standard test, the indirect immunoperoxidase assay (IIP). Control serum samples were collected from 96 American soldiers and 198 Royal Thai Army soldiers with no recent history of clinical illness. Sera were examined from 79 febrile, Thai scrub typhus patients presenting at Chiang Rai (76) and Bangkraui Nontaburi (3) Provincial hospitals (cases confirmed by elevated IIP IgG levels > or = 1:1,600, IgM levels > or = 1:400, or presence of an eschar). The mean + 2 SD, used for the upper limit of normal reactions in the IgG ELISA, was 0.10 for U.S. soldiers and 0.42 for Thai soldiers. Using the 0.10 cutoff value, 29% of the asymptomatic Thai soldiers would be designated as antibody positive. Variability of IgG ELISA values was greater in the Thai soldier group than in American soldiers, possibly reflecting previous exposure to O. tsutsugamushi. In the Thai patients, there was a significant correlation between IIP titers and single serum dilution (1:100) ELISA values (IgG, r = 0.75, n = 104; P < 0.0005; IgM, r = 0.70, n = 75; P < 0.0005) and between IIP titers and ELISA titers (IgG, r = 0.87, n = 103; P < 0.0005; IgM, r = 0.76, n = 75; P < 0.0005). The single serum dilution ELISA was as effective as the titration in determining presence of specific antibodies. The O. tsutsugamushi ELISA is a rapid and objective test amenable to accurately testing the large numbers of sera often obtained in seroepidemiologic investigations. SN - 0002-9637 UR - https://www.unboundmedicine.com/medline/citation/9063359/Evaluation_of_an_enzyme_linked_immunosorbent_assay_in_Thai_scrub_typhus_patients_ L2 - https://ajtmh.org/doi/10.4269/ajtmh.1997.56.38 DB - PRIME DP - Unbound Medicine ER -