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Epidermal growth factor selectively enhances functional enterocyte adaptation after massive small bowel resection.
J Surg Res. 1997 Jan; 67(1):90-3.JS

Abstract

After massive small bowel resection, the intestine adapts to compensate. In addition to proliferation, enterocytes also undergo selective functional adaptation. In this study we examined the effect of intraperitoneal administration of epidermal growth factor (EGF) on the expression of the brush border dissacharidase sucrase, the sodium glucose cotransporter (SGLT1), and the sodium-potassium ATPase pump (NaK ATPase) by enterocytes in the remnant intestine after massive small bowel resection. Adult Lewis rats underwent either ileal transection or 70% proximal intestinal resection. These animals were subdivided into groups that received either saline or EGF intraperitoneally for 1 week. Ilea from each group were harvested 4 weeks postoperatively. Enterocytes were separated from these segments by calcium chelation. The total protein from the isolated cells was subjected to Western blot analysis. Administration of EGF to animals that underwent transection did not significantly alter the expression of sucrase, SGLT1, or NaK ATPase. After intestinal resection, the expressions of sucrase and SGLT1 were significantly increased. The combination of EGF administration and intestinal resection resulted in a further increase in SGLT1 expression. The intraperitoneal administration of EGF selectively enhanced the expression of SGLT1 by enterocytes after massive small bowel resection. Administration of EGF to sham-operated animals did not have similar effects. These results suggest that EGF augments the adaptive response and may therefore have a therapeutic role in the management of patients with short bowel syndrome.

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Authors+Show Affiliations

Dunn JC
Department of Surgery, Sepulveda Veterans Administration Medical Center, Los Angeles, California, USA.
Parungo CP
No affiliation info available
Fonkalsrud EW
No affiliation info available
McFadden DW
No affiliation info available
Ashley SW
No affiliation info available

MeSH

Adaptation, PhysiologicalAnimalsBlotting, WesternDNAEpidermal Growth FactorFemaleIleumIntestine, SmallMembrane GlycoproteinsMonosaccharide Transport ProteinsProteinsRatsRats, Inbred LewSodium-Glucose Transporter 1Sodium-Potassium-Exchanging ATPaseSucrase

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9070188

Citation

Dunn, J C., et al. "Epidermal Growth Factor Selectively Enhances Functional Enterocyte Adaptation After Massive Small Bowel Resection." The Journal of Surgical Research, vol. 67, no. 1, 1997, pp. 90-3.
Dunn JC, Parungo CP, Fonkalsrud EW, et al. Epidermal growth factor selectively enhances functional enterocyte adaptation after massive small bowel resection. J Surg Res. 1997;67(1):90-3.
Dunn, J. C., Parungo, C. P., Fonkalsrud, E. W., McFadden, D. W., & Ashley, S. W. (1997). Epidermal growth factor selectively enhances functional enterocyte adaptation after massive small bowel resection. The Journal of Surgical Research, 67(1), 90-3.
Dunn JC, et al. Epidermal Growth Factor Selectively Enhances Functional Enterocyte Adaptation After Massive Small Bowel Resection. J Surg Res. 1997;67(1):90-3. PubMed PMID: 9070188.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epidermal growth factor selectively enhances functional enterocyte adaptation after massive small bowel resection. AU - Dunn,J C, AU - Parungo,C P, AU - Fonkalsrud,E W, AU - McFadden,D W, AU - Ashley,S W, PY - 1997/1/1/pubmed PY - 1997/1/1/medline PY - 1997/1/1/entrez SP - 90 EP - 3 JF - The Journal of surgical research JO - J Surg Res VL - 67 IS - 1 N2 - After massive small bowel resection, the intestine adapts to compensate. In addition to proliferation, enterocytes also undergo selective functional adaptation. In this study we examined the effect of intraperitoneal administration of epidermal growth factor (EGF) on the expression of the brush border dissacharidase sucrase, the sodium glucose cotransporter (SGLT1), and the sodium-potassium ATPase pump (NaK ATPase) by enterocytes in the remnant intestine after massive small bowel resection. Adult Lewis rats underwent either ileal transection or 70% proximal intestinal resection. These animals were subdivided into groups that received either saline or EGF intraperitoneally for 1 week. Ilea from each group were harvested 4 weeks postoperatively. Enterocytes were separated from these segments by calcium chelation. The total protein from the isolated cells was subjected to Western blot analysis. Administration of EGF to animals that underwent transection did not significantly alter the expression of sucrase, SGLT1, or NaK ATPase. After intestinal resection, the expressions of sucrase and SGLT1 were significantly increased. The combination of EGF administration and intestinal resection resulted in a further increase in SGLT1 expression. The intraperitoneal administration of EGF selectively enhanced the expression of SGLT1 by enterocytes after massive small bowel resection. Administration of EGF to sham-operated animals did not have similar effects. These results suggest that EGF augments the adaptive response and may therefore have a therapeutic role in the management of patients with short bowel syndrome. SN - 0022-4804 UR - https://www.unboundmedicine.com/medline/citation/9070188/Epidermal_growth_factor_selectively_enhances_functional_enterocyte_adaptation_after_massive_small_bowel_resection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(96)94959-8 DB - PRIME DP - Unbound Medicine ER -