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Mucosal transmission of cell-associated and cell-free feline immunodeficiency virus.
AIDS Res Hum Retroviruses. 1997 Mar 01; 13(4):347-55.AR

Abstract

Mucosal infection by feline immunodeficiency virus (FIV) was assessed via a single exposure of the vaginal or rectal mucosa to either infectious peripheral blood mononuclear cells (PBMCs), infectious plasma, or cell-free cultured virus. All cats inoculated with cell-free cultured virus (100 or 400 TCID) and 9 of 10 cats inoculated with infected PBMCs (2 x 10(7) or 2 x 10(5)) became persistently viremic within 3 weeks. Neither cat inoculated with 2 x 10(3) PBMCs became viremic. Rectal and vaginal exposure were equally effective routes to induce viremia. CD4+ T cells and mitogen-stimulated PBMC proliferation declined in all infected cats. However, a transient PBMC proliferative response to FIV p24gag occurred in most virus-exposed cats, especially those that did not develop detectable infection. FIV was not transmitted by mucosal exposure to infectious virus in plasma (100 TCID), a dose > 10-fold that needed for infection by parental injection. In vitro studies suggested that a plasma heat-stable virus-neutralizing factor may be associated with failure of plasma virus to establish infection via the mucosal route. Mucosal FIV infection provides a new model with which to study early stages of infection and intervention in transmucosal lentivirus infections.

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Authors+Show Affiliations

Burkhard MJ
Department of Pathology, College of Veterinary Medicine, Colorado State University, Fort Collins 80523, USA.
Obert LA
No affiliation info available
O'Neil LL
No affiliation info available
Diehl LJ
No affiliation info available
Hoover EA
No affiliation info available

MeSH

AnimalsAntibodies, ViralCatsCell-Free SystemFemaleHeatingImmunodeficiency Virus, FelineIn Situ HybridizationLentivirus InfectionsLymphoproliferative DisordersMaleMitogensPolymerase Chain ReactionRectumT-Lymphocyte SubsetsVagina

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9071435

Citation

Burkhard, M J., et al. "Mucosal Transmission of Cell-associated and Cell-free Feline Immunodeficiency Virus." AIDS Research and Human Retroviruses, vol. 13, no. 4, 1997, pp. 347-55.
Burkhard MJ, Obert LA, O'Neil LL, et al. Mucosal transmission of cell-associated and cell-free feline immunodeficiency virus. AIDS Res Hum Retroviruses. 1997;13(4):347-55.
Burkhard, M. J., Obert, L. A., O'Neil, L. L., Diehl, L. J., & Hoover, E. A. (1997). Mucosal transmission of cell-associated and cell-free feline immunodeficiency virus. AIDS Research and Human Retroviruses, 13(4), 347-55.
Burkhard MJ, et al. Mucosal Transmission of Cell-associated and Cell-free Feline Immunodeficiency Virus. AIDS Res Hum Retroviruses. 1997 Mar 1;13(4):347-55. PubMed PMID: 9071435.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mucosal transmission of cell-associated and cell-free feline immunodeficiency virus. AU - Burkhard,M J, AU - Obert,L A, AU - O'Neil,L L, AU - Diehl,L J, AU - Hoover,E A, PY - 1997/3/1/pubmed PY - 1997/3/1/medline PY - 1997/3/1/entrez SP - 347 EP - 55 JF - AIDS research and human retroviruses JO - AIDS Res Hum Retroviruses VL - 13 IS - 4 N2 - Mucosal infection by feline immunodeficiency virus (FIV) was assessed via a single exposure of the vaginal or rectal mucosa to either infectious peripheral blood mononuclear cells (PBMCs), infectious plasma, or cell-free cultured virus. All cats inoculated with cell-free cultured virus (100 or 400 TCID) and 9 of 10 cats inoculated with infected PBMCs (2 x 10(7) or 2 x 10(5)) became persistently viremic within 3 weeks. Neither cat inoculated with 2 x 10(3) PBMCs became viremic. Rectal and vaginal exposure were equally effective routes to induce viremia. CD4+ T cells and mitogen-stimulated PBMC proliferation declined in all infected cats. However, a transient PBMC proliferative response to FIV p24gag occurred in most virus-exposed cats, especially those that did not develop detectable infection. FIV was not transmitted by mucosal exposure to infectious virus in plasma (100 TCID), a dose > 10-fold that needed for infection by parental injection. In vitro studies suggested that a plasma heat-stable virus-neutralizing factor may be associated with failure of plasma virus to establish infection via the mucosal route. Mucosal FIV infection provides a new model with which to study early stages of infection and intervention in transmucosal lentivirus infections. SN - 0889-2229 UR - https://www.unboundmedicine.com/medline/citation/9071435/Mucosal_transmission_of_cell_associated_and_cell_free_feline_immunodeficiency_virus_ L2 - https://www.liebertpub.com/doi/10.1089/aid.1997.13.347?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -