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Variable efficiency of a Ti plasmid-encoded VirA protein in different agrobacterial hosts.
J Bacteriol. 1997 Apr; 179(7):2305-13.JB

Abstract

The transconjugant CB100, harboring the Ti plasmid from the Agrobacterium tumefaciens biovar 2 strain D10B/87 in the chromosomal background of the biovar 1 strain C58, was defective in vir gene induction. This defect was corrected in the presence of virA from pTiA6. Based on this complementation result and an analysis of the induction requirements of the transconjugant CB100 and its parent strains, it was hypothesized that the defective vir gene induction in CB100 was related to a dysfunctional interaction between the pTi-encoded D10B/87 VirA and the chromosome-encoded C58 ChvE. To verify this hypothesis, D10B/87 and C58 virA were compared, and conclusions from this first set of analyses were then corroborated by comparing D10B/87 and C58 chvE. Whereas only a few nucleotide differences were identified in the promoters and 5' ends of the coding regions of D10B/87 and C58 virA, analysis of hybrid virA genes showed that these differences collectively accounted for the poor vir gene induction of strain CB100. In contrast with the sequence similarity of the VirA proteins, extensive divergence was seen between the chromosome-encoded D10B/87 and C58 ChvE. Although D10B/87 chvE introduced in trans had little effect on vir gene induction of CB100, it enhanced the induction response of a strain CB100 derivative in which the chromosomal C58 chvE had been inactivated by marker exchange. These results suggest that chromosomal backgrounds provided by different strains of A. tumefaciens are not equivalent for VirA function. Following conjugative transfer of certain Ti plasmids to a new agrobacterial host, evolution of the newly introduced virA, or coevolution of chvE and virA, may lead to optimization of ChvE-VirA interaction and vir gene induction levels.

Authors+Show Affiliations

Recherche en Sciences de la Vie et de la Santé, Université Laval, Sainte-Foy, Québec, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9079917

Citation

Bélanger, C, et al. "Variable Efficiency of a Ti Plasmid-encoded VirA Protein in Different Agrobacterial Hosts." Journal of Bacteriology, vol. 179, no. 7, 1997, pp. 2305-13.
Bélanger C, Loubens I, Nester EW, et al. Variable efficiency of a Ti plasmid-encoded VirA protein in different agrobacterial hosts. J Bacteriol. 1997;179(7):2305-13.
Bélanger, C., Loubens, I., Nester, E. W., & Dion, P. (1997). Variable efficiency of a Ti plasmid-encoded VirA protein in different agrobacterial hosts. Journal of Bacteriology, 179(7), 2305-13.
Bélanger C, et al. Variable Efficiency of a Ti Plasmid-encoded VirA Protein in Different Agrobacterial Hosts. J Bacteriol. 1997;179(7):2305-13. PubMed PMID: 9079917.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Variable efficiency of a Ti plasmid-encoded VirA protein in different agrobacterial hosts. AU - Bélanger,C, AU - Loubens,I, AU - Nester,E W, AU - Dion,P, PY - 1997/4/1/pubmed PY - 1997/4/1/medline PY - 1997/4/1/entrez SP - 2305 EP - 13 JF - Journal of bacteriology JO - J. Bacteriol. VL - 179 IS - 7 N2 - The transconjugant CB100, harboring the Ti plasmid from the Agrobacterium tumefaciens biovar 2 strain D10B/87 in the chromosomal background of the biovar 1 strain C58, was defective in vir gene induction. This defect was corrected in the presence of virA from pTiA6. Based on this complementation result and an analysis of the induction requirements of the transconjugant CB100 and its parent strains, it was hypothesized that the defective vir gene induction in CB100 was related to a dysfunctional interaction between the pTi-encoded D10B/87 VirA and the chromosome-encoded C58 ChvE. To verify this hypothesis, D10B/87 and C58 virA were compared, and conclusions from this first set of analyses were then corroborated by comparing D10B/87 and C58 chvE. Whereas only a few nucleotide differences were identified in the promoters and 5' ends of the coding regions of D10B/87 and C58 virA, analysis of hybrid virA genes showed that these differences collectively accounted for the poor vir gene induction of strain CB100. In contrast with the sequence similarity of the VirA proteins, extensive divergence was seen between the chromosome-encoded D10B/87 and C58 ChvE. Although D10B/87 chvE introduced in trans had little effect on vir gene induction of CB100, it enhanced the induction response of a strain CB100 derivative in which the chromosomal C58 chvE had been inactivated by marker exchange. These results suggest that chromosomal backgrounds provided by different strains of A. tumefaciens are not equivalent for VirA function. Following conjugative transfer of certain Ti plasmids to a new agrobacterial host, evolution of the newly introduced virA, or coevolution of chvE and virA, may lead to optimization of ChvE-VirA interaction and vir gene induction levels. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/9079917/Variable_efficiency_of_a_Ti_plasmid_encoded_VirA_protein_in_different_agrobacterial_hosts_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=9079917 DB - PRIME DP - Unbound Medicine ER -