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Characterization of ciguatoxins and ciguatoxin congeners present in ciguateric fish by gradient reverse-phase high-performance liquid chromatography/mass spectrometry.
Toxicon. 1997 Feb; 35(2):159-68.T

Abstract

Reverse-phase high-performance liquid chromatography/mass spectrometry (HPLC/MS) was used to identify Pacific ciguatoxins (P-CTX) and P-CTX congeners present in a highly purified extract from the viscera of ciguateric moray eels (Lycodontis javanicus) collected in the central Pacific Ocean. Fourteen P-CTX or P-CTX congeners were identified with protonated molecular ions [M + H]+ m/z 1095.7 (two), 1111.6 (six) or 1127.7 (six), including dominant ions for P-CTX-1, -2 and -3. In addition to the protonated species, each of these ciguatoxins gave rise to prominent [M + NH4]+ and [M + Na]+ ions. The 11 new P-CTX congeners, not readily detected by mouse bioassay, were present in trace amounts (2-13% of P-CTX-2 levels) and identified as several oxidized P-CTX-1, -2 and -3, and a possible diasteriomer of P-CTX-1. Acetonitrile-water gradients buffered with 1 mM ammonium acetate improved the separation and detection of the minor ciguatoxins compared with an acetonitrile-water gradient modified with 0.1% TFA. Turbo-assisted HPLC/MS had sufficient sensitivity to detect P-CTX-1 in a crude extract from the flesh of an Australian ciguateric fish. Compounds with masses equivalent to other isolated ciguatoxins, including Caribbean-CTX-1, gambiertoxin-4A and P-CTX-3C, were not detected in these samples. HPLC/MS can readily identify multiple ciguatoxins accumulated by fish and has the potential to be used as a confirmatory analytical method for characterizing the low levels of ciguatoxins contaminating ciguateric fish.

Authors+Show Affiliations

Queensland Agricultural Biotechnology Centre (QDPI), University of Queensland, Brisbane, Australia.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9080572

Citation

Lewis, R J., and A Jones. "Characterization of Ciguatoxins and Ciguatoxin Congeners Present in Ciguateric Fish By Gradient Reverse-phase High-performance Liquid Chromatography/mass Spectrometry." Toxicon : Official Journal of the International Society On Toxinology, vol. 35, no. 2, 1997, pp. 159-68.
Lewis RJ, Jones A. Characterization of ciguatoxins and ciguatoxin congeners present in ciguateric fish by gradient reverse-phase high-performance liquid chromatography/mass spectrometry. Toxicon. 1997;35(2):159-68.
Lewis, R. J., & Jones, A. (1997). Characterization of ciguatoxins and ciguatoxin congeners present in ciguateric fish by gradient reverse-phase high-performance liquid chromatography/mass spectrometry. Toxicon : Official Journal of the International Society On Toxinology, 35(2), 159-68.
Lewis RJ, Jones A. Characterization of Ciguatoxins and Ciguatoxin Congeners Present in Ciguateric Fish By Gradient Reverse-phase High-performance Liquid Chromatography/mass Spectrometry. Toxicon. 1997;35(2):159-68. PubMed PMID: 9080572.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of ciguatoxins and ciguatoxin congeners present in ciguateric fish by gradient reverse-phase high-performance liquid chromatography/mass spectrometry. AU - Lewis,R J, AU - Jones,A, PY - 1997/2/1/pubmed PY - 1997/2/1/medline PY - 1997/2/1/entrez SP - 159 EP - 68 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 35 IS - 2 N2 - Reverse-phase high-performance liquid chromatography/mass spectrometry (HPLC/MS) was used to identify Pacific ciguatoxins (P-CTX) and P-CTX congeners present in a highly purified extract from the viscera of ciguateric moray eels (Lycodontis javanicus) collected in the central Pacific Ocean. Fourteen P-CTX or P-CTX congeners were identified with protonated molecular ions [M + H]+ m/z 1095.7 (two), 1111.6 (six) or 1127.7 (six), including dominant ions for P-CTX-1, -2 and -3. In addition to the protonated species, each of these ciguatoxins gave rise to prominent [M + NH4]+ and [M + Na]+ ions. The 11 new P-CTX congeners, not readily detected by mouse bioassay, were present in trace amounts (2-13% of P-CTX-2 levels) and identified as several oxidized P-CTX-1, -2 and -3, and a possible diasteriomer of P-CTX-1. Acetonitrile-water gradients buffered with 1 mM ammonium acetate improved the separation and detection of the minor ciguatoxins compared with an acetonitrile-water gradient modified with 0.1% TFA. Turbo-assisted HPLC/MS had sufficient sensitivity to detect P-CTX-1 in a crude extract from the flesh of an Australian ciguateric fish. Compounds with masses equivalent to other isolated ciguatoxins, including Caribbean-CTX-1, gambiertoxin-4A and P-CTX-3C, were not detected in these samples. HPLC/MS can readily identify multiple ciguatoxins accumulated by fish and has the potential to be used as a confirmatory analytical method for characterizing the low levels of ciguatoxins contaminating ciguateric fish. SN - 0041-0101 UR - https://www.unboundmedicine.com/medline/citation/9080572/Characterization_of_ciguatoxins_and_ciguatoxin_congeners_present_in_ciguateric_fish_by_gradient_reverse_phase_high_performance_liquid_chromatography/mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041010196001328 DB - PRIME DP - Unbound Medicine ER -