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Replication of plasmids with the p15A origin in Shewanella putrefaciens MR-1.
Lett Appl Microbiol 1997; 24(3):221-5LA

Abstract

The plasmid pACYC184 was introduced into Shewanella putrefaciens MR-1 by electroporation. In 100% of the transformants examined, the plasmid was maintained as a free replicon outside the chromosome. This was the case whether or not the plasmid contained a 224-bp DNA insert derived from an open-reading frame of MR-1 genomic DNA. Therefore, in contrast to a report in the literature, plasmids containing the p15A origin of replication can replicate freely in S. putrefaciens MR-1, and do not make convenient vectors for gene replacement in this bacterium. However, we found that plasmids with the pMB1 origin of replication (e.g. pBR322) cannot replicate in MR-1 and could therefore have potential as vectors for gene replacement.

Authors+Show Affiliations

Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee 53226, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9080705

Citation

Myers, C R., and J M. Myers. "Replication of Plasmids With the p15A Origin in Shewanella Putrefaciens MR-1." Letters in Applied Microbiology, vol. 24, no. 3, 1997, pp. 221-5.
Myers CR, Myers JM. Replication of plasmids with the p15A origin in Shewanella putrefaciens MR-1. Lett Appl Microbiol. 1997;24(3):221-5.
Myers, C. R., & Myers, J. M. (1997). Replication of plasmids with the p15A origin in Shewanella putrefaciens MR-1. Letters in Applied Microbiology, 24(3), pp. 221-5.
Myers CR, Myers JM. Replication of Plasmids With the p15A Origin in Shewanella Putrefaciens MR-1. Lett Appl Microbiol. 1997;24(3):221-5. PubMed PMID: 9080705.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Replication of plasmids with the p15A origin in Shewanella putrefaciens MR-1. AU - Myers,C R, AU - Myers,J M, PY - 1997/3/1/pubmed PY - 1997/3/1/medline PY - 1997/3/1/entrez SP - 221 EP - 5 JF - Letters in applied microbiology JO - Lett. Appl. Microbiol. VL - 24 IS - 3 N2 - The plasmid pACYC184 was introduced into Shewanella putrefaciens MR-1 by electroporation. In 100% of the transformants examined, the plasmid was maintained as a free replicon outside the chromosome. This was the case whether or not the plasmid contained a 224-bp DNA insert derived from an open-reading frame of MR-1 genomic DNA. Therefore, in contrast to a report in the literature, plasmids containing the p15A origin of replication can replicate freely in S. putrefaciens MR-1, and do not make convenient vectors for gene replacement in this bacterium. However, we found that plasmids with the pMB1 origin of replication (e.g. pBR322) cannot replicate in MR-1 and could therefore have potential as vectors for gene replacement. SN - 0266-8254 UR - https://www.unboundmedicine.com/medline/citation/9080705/Replication_of_plasmids_with_the_p15A_origin_in_Shewanella_putrefaciens_MR_1_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0266-8254&date=1997&volume=24&issue=3&spage=221 DB - PRIME DP - Unbound Medicine ER -