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Carboxyhaemoglobin. Spectrophotometric determination tested and calibrated using a new reference method for measuring carbon monoxide in blood.
Clin Chim Acta. 1977 Oct 01; 80(1):95-104.CC

Abstract

The spectrophotometric determination of HbCO at lambda = 562 and 540 nm in the system HbCO/HbO2 was reinvestigated using a new reference method for measuring CO in blood. This reference method is based on the conversion of CO from HbCO into CO2 which is determined by titration. Plotting the absorbance ratio A562/A540 against the titrimetrically determined HbCO fractions of 46 blood samples demonstrated a linear relationship up to 90% HbCO and yielded more accurate values for the constants in the equation for calculating the HbCO fraction from A562/A540. The standard deviation of the differences between the spectrophotometric and the titrimetric method was 1.2% HbCO. It is shown that the influence of other haemoglobin derivatives and other possible sources of error is either negligible or can be prevented by simple precautions.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

908149

Citation

Dijkhuizen, P, et al. "Carboxyhaemoglobin. Spectrophotometric Determination Tested and Calibrated Using a New Reference Method for Measuring Carbon Monoxide in Blood." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 80, no. 1, 1977, pp. 95-104.
Dijkhuizen P, Buursma A, Gerding AM, et al. Carboxyhaemoglobin. Spectrophotometric determination tested and calibrated using a new reference method for measuring carbon monoxide in blood. Clin Chim Acta. 1977;80(1):95-104.
Dijkhuizen, P., Buursma, A., Gerding, A. M., Van Kampen, E. J., & Zijlstra, W. G. (1977). Carboxyhaemoglobin. Spectrophotometric determination tested and calibrated using a new reference method for measuring carbon monoxide in blood. Clinica Chimica Acta; International Journal of Clinical Chemistry, 80(1), 95-104.
Dijkhuizen P, et al. Carboxyhaemoglobin. Spectrophotometric Determination Tested and Calibrated Using a New Reference Method for Measuring Carbon Monoxide in Blood. Clin Chim Acta. 1977 Oct 1;80(1):95-104. PubMed PMID: 908149.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carboxyhaemoglobin. Spectrophotometric determination tested and calibrated using a new reference method for measuring carbon monoxide in blood. AU - Dijkhuizen,P, AU - Buursma,A, AU - Gerding,A M, AU - Van Kampen,E J, AU - Zijlstra,W G, PY - 1977/10/1/pubmed PY - 1977/10/1/medline PY - 1977/10/1/entrez SP - 95 EP - 104 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin Chim Acta VL - 80 IS - 1 N2 - The spectrophotometric determination of HbCO at lambda = 562 and 540 nm in the system HbCO/HbO2 was reinvestigated using a new reference method for measuring CO in blood. This reference method is based on the conversion of CO from HbCO into CO2 which is determined by titration. Plotting the absorbance ratio A562/A540 against the titrimetrically determined HbCO fractions of 46 blood samples demonstrated a linear relationship up to 90% HbCO and yielded more accurate values for the constants in the equation for calculating the HbCO fraction from A562/A540. The standard deviation of the differences between the spectrophotometric and the titrimetric method was 1.2% HbCO. It is shown that the influence of other haemoglobin derivatives and other possible sources of error is either negligible or can be prevented by simple precautions. SN - 0009-8981 UR - https://www.unboundmedicine.com/medline/citation/908149/Carboxyhaemoglobin__Spectrophotometric_determination_tested_and_calibrated_using_a_new_reference_method_for_measuring_carbon_monoxide_in_blood_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0009-8981(77)90268-6 DB - PRIME DP - Unbound Medicine ER -