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Adherence of nontypable Haemophilus influenzae to respiratory epithelium of otitis prone and normal children.
Acta Otolaryngol Suppl. 1996; 523:142-4.AO

Abstract

The present study was designed to determine whether nontypable H. influenzae adhered differently to buccal cells from otitis prone compared with normal children. 306 children were enrolled at birth. They were examined at 1-6, 8, 10, 12, 15, 18, 21, and 24 months of age. Buccal cells were collected at 1, 12, and 24 months. 40 children were classified as OP (> or = 4 episodes by age 12 months or > or = 6 episodes by age 24 months). Adherence assays were conducted by incubating 1 x 10(4) buccal cells with 5 x 10(8) colony forming units of radiolabelled nontypable H. influenzae in 1 ml of salt solution with 0.1% gel at 37 degrees C for 60 min. Adherence was expressed as the % of organisms adherent to buccal cells. The adherence of buccal cells from otitis prone children were compared with age and sex matched controls who had not experienced otitis media and had not been colonized with nontypable H. influenzae.

Authors+Show Affiliations

State University of New York at Buffalo, Department of Pediatrics, Buffalo, New York, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9082762

Citation

Faden, H, et al. "Adherence of Nontypable Haemophilus Influenzae to Respiratory Epithelium of Otitis Prone and Normal Children." Acta Oto-laryngologica. Supplementum, vol. 523, 1996, pp. 142-4.
Faden H, Duffy L, Hong JJ. Adherence of nontypable Haemophilus influenzae to respiratory epithelium of otitis prone and normal children. Acta Otolaryngol Suppl. 1996;523:142-4.
Faden, H., Duffy, L., & Hong, J. J. (1996). Adherence of nontypable Haemophilus influenzae to respiratory epithelium of otitis prone and normal children. Acta Oto-laryngologica. Supplementum, 523, 142-4.
Faden H, Duffy L, Hong JJ. Adherence of Nontypable Haemophilus Influenzae to Respiratory Epithelium of Otitis Prone and Normal Children. Acta Otolaryngol Suppl. 1996;523:142-4. PubMed PMID: 9082762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adherence of nontypable Haemophilus influenzae to respiratory epithelium of otitis prone and normal children. AU - Faden,H, AU - Duffy,L, AU - Hong,J J, PY - 1996/1/1/pubmed PY - 1996/1/1/medline PY - 1996/1/1/entrez SP - 142 EP - 4 JF - Acta oto-laryngologica. Supplementum JO - Acta Otolaryngol Suppl VL - 523 N2 - The present study was designed to determine whether nontypable H. influenzae adhered differently to buccal cells from otitis prone compared with normal children. 306 children were enrolled at birth. They were examined at 1-6, 8, 10, 12, 15, 18, 21, and 24 months of age. Buccal cells were collected at 1, 12, and 24 months. 40 children were classified as OP (> or = 4 episodes by age 12 months or > or = 6 episodes by age 24 months). Adherence assays were conducted by incubating 1 x 10(4) buccal cells with 5 x 10(8) colony forming units of radiolabelled nontypable H. influenzae in 1 ml of salt solution with 0.1% gel at 37 degrees C for 60 min. Adherence was expressed as the % of organisms adherent to buccal cells. The adherence of buccal cells from otitis prone children were compared with age and sex matched controls who had not experienced otitis media and had not been colonized with nontypable H. influenzae. SN - 0365-5237 UR - https://www.unboundmedicine.com/medline/citation/9082762/Adherence_of_nontypable_Haemophilus_influenzae_to_respiratory_epithelium_of_otitis_prone_and_normal_children_ L2 - http://www.diseaseinfosearch.org/result/3211 DB - PRIME DP - Unbound Medicine ER -