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Detection of genitourinary tract Chlamydia trachomatis infection in pregnant women by ligase chain reaction assay.
Obstet Gynecol. 1997 Apr; 89(4):556-60.OG

Abstract

OBJECTIVE

To compare the sensitivity and specificity of a ligase chain reaction assay of cervical swabs and voided urine with those of cervical swab tissue culture for the detection of genitourinary tract infection with Chlamydia trachomatis in pregnant women.

METHODS

Infection with C trachomatis was assessed in cervical swabs by culture and in both cervical swabs and voided urine specimens by a ligase chain reaction assay specific for C trachomatis plasmid DNA. The matched cervical swab and voided urine specimens were collected from 462 women during routine visits to prenatal clinics. Standard criteria that defined infection included: 1) a positive cervical culture result or 2) a negative culture but a positive ligase chain reaction result in either the urine or cervical specimen that was confirmed by supplementary testing. Test performance was assessed by determination of sensitivity and specificity, and differences in paired results were determined using McNemar analysis.

RESULTS

The prevalence of genitourinary C trachomatis infection was 6.1% (n = 28) by cervical culture (sensitivity 30.1%; specificity 100%), 18.2% (n = 84) by ligase chain reaction of cervical swabs (sensitivity 90.3%; specificity 100%), and 16.9% (n = 78) by ligase chain reaction of urine (sensitivity 83.9%; specificity 99.5%). Relative to the number of women with a positive culture or a confirmed ligase chain reaction-positive cervical swab, the sensitivity and specificity were 82.8% and 97.9%, respectively, for ligase chain reaction of urine and 96.6% and 100%, respectively, for ligase chain reaction of cervical swabs. Ligase chain reaction of cervical swabs and urine detected 89.3% and 82.1%, respectively, of women with a positive cervical culture.

CONCLUSIONS

Ligase chain reaction assay of cervical or urine specimens detected considerably more pregnant women with C trachomatis infection of the genitourinary tract than did cervical culture. Ligase chain reaction testing of urine is a simple and effective means of screening pregnant women for genitourinary tract infection with C trachomatis.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, University of Alabama at Birmingham 35233-7333, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

9083312

Citation

Andrews, W W., et al. "Detection of Genitourinary Tract Chlamydia Trachomatis Infection in Pregnant Women By Ligase Chain Reaction Assay." Obstetrics and Gynecology, vol. 89, no. 4, 1997, pp. 556-60.
Andrews WW, Lee HH, Roden WJ, et al. Detection of genitourinary tract Chlamydia trachomatis infection in pregnant women by ligase chain reaction assay. Obstet Gynecol. 1997;89(4):556-60.
Andrews, W. W., Lee, H. H., Roden, W. J., & Mott, C. W. (1997). Detection of genitourinary tract Chlamydia trachomatis infection in pregnant women by ligase chain reaction assay. Obstetrics and Gynecology, 89(4), 556-60.
Andrews WW, et al. Detection of Genitourinary Tract Chlamydia Trachomatis Infection in Pregnant Women By Ligase Chain Reaction Assay. Obstet Gynecol. 1997;89(4):556-60. PubMed PMID: 9083312.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of genitourinary tract Chlamydia trachomatis infection in pregnant women by ligase chain reaction assay. AU - Andrews,W W, AU - Lee,H H, AU - Roden,W J, AU - Mott,C W, PY - 1997/4/1/pubmed PY - 1997/4/1/medline PY - 1997/4/1/entrez SP - 556 EP - 60 JF - Obstetrics and gynecology JO - Obstet Gynecol VL - 89 IS - 4 N2 - OBJECTIVE: To compare the sensitivity and specificity of a ligase chain reaction assay of cervical swabs and voided urine with those of cervical swab tissue culture for the detection of genitourinary tract infection with Chlamydia trachomatis in pregnant women. METHODS: Infection with C trachomatis was assessed in cervical swabs by culture and in both cervical swabs and voided urine specimens by a ligase chain reaction assay specific for C trachomatis plasmid DNA. The matched cervical swab and voided urine specimens were collected from 462 women during routine visits to prenatal clinics. Standard criteria that defined infection included: 1) a positive cervical culture result or 2) a negative culture but a positive ligase chain reaction result in either the urine or cervical specimen that was confirmed by supplementary testing. Test performance was assessed by determination of sensitivity and specificity, and differences in paired results were determined using McNemar analysis. RESULTS: The prevalence of genitourinary C trachomatis infection was 6.1% (n = 28) by cervical culture (sensitivity 30.1%; specificity 100%), 18.2% (n = 84) by ligase chain reaction of cervical swabs (sensitivity 90.3%; specificity 100%), and 16.9% (n = 78) by ligase chain reaction of urine (sensitivity 83.9%; specificity 99.5%). Relative to the number of women with a positive culture or a confirmed ligase chain reaction-positive cervical swab, the sensitivity and specificity were 82.8% and 97.9%, respectively, for ligase chain reaction of urine and 96.6% and 100%, respectively, for ligase chain reaction of cervical swabs. Ligase chain reaction of cervical swabs and urine detected 89.3% and 82.1%, respectively, of women with a positive cervical culture. CONCLUSIONS: Ligase chain reaction assay of cervical or urine specimens detected considerably more pregnant women with C trachomatis infection of the genitourinary tract than did cervical culture. Ligase chain reaction testing of urine is a simple and effective means of screening pregnant women for genitourinary tract infection with C trachomatis. SN - 0029-7844 UR - https://www.unboundmedicine.com/medline/citation/9083312/Detection_of_genitourinary_tract_Chlamydia_trachomatis_infection_in_pregnant_women_by_ligase_chain_reaction_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0029-7844(97)00003-3 DB - PRIME DP - Unbound Medicine ER -