Tags

Type your tag names separated by a space and hit enter

Expression of the hematopoietic stem cell antigen CD34 on blood and bone marrow monoclonal plasma cells from patients with multiple myeloma.
Bone Marrow Transplant. 1997 Mar; 19(6):553-6.BM

Abstract

Monoclonal plasma cells (CD38+CD45-/dim) are typically present in the blood of patients with active myeloma and can contaminate stem cell harvests. This has led to strategies that select CD34+ cells for use in autologous stem cell transplantation with the goal of decreasing tumor cell contamination. The aim of this study was to learn if the CD34 antigen is expressed on monoclonal plasma cells in the blood or marrow of patients with multiple myeloma. We used three-color flow cytometry (surface CD38;CD45 and cytoplasmic kappa or lambda) to identify monoclonal plasma cells in the blood (n = 24) and marrow (n = 37) from patients with plasma cell proliferative disorders. In each case the CD38+CD45- and CD38+CD45dim+ monoclonal populations were then analyzed for CD34 expression. In all 24 blood and 37 marrow samples, the CD38+CD45-monoclonal plasma cells were negative for CD34 expression. CD38+CD45dim+ monoclonal cells were documented in the blood of 11 patients and in the marrow of 33 patients and this cell population was also CD34-negative in all cases. These results indicate that CD34 is usually not expressed on the CD38+CD45-CD45dim+ monoclonal plasma cells in the blood or marrow of patients with plasma cell proliferative disorders. CD34 selection methods should therefore decrease the chance of tumor cell contamination of the stem cell product.

Authors+Show Affiliations

Department of Laboratory Medicine, Mayo Clinic, Rochester, MN 55905, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9085734

Citation

Kimlinger, T, and T E. Witzig. "Expression of the Hematopoietic Stem Cell Antigen CD34 On Blood and Bone Marrow Monoclonal Plasma Cells From Patients With Multiple Myeloma." Bone Marrow Transplantation, vol. 19, no. 6, 1997, pp. 553-6.
Kimlinger T, Witzig TE. Expression of the hematopoietic stem cell antigen CD34 on blood and bone marrow monoclonal plasma cells from patients with multiple myeloma. Bone Marrow Transplant. 1997;19(6):553-6.
Kimlinger, T., & Witzig, T. E. (1997). Expression of the hematopoietic stem cell antigen CD34 on blood and bone marrow monoclonal plasma cells from patients with multiple myeloma. Bone Marrow Transplantation, 19(6), 553-6.
Kimlinger T, Witzig TE. Expression of the Hematopoietic Stem Cell Antigen CD34 On Blood and Bone Marrow Monoclonal Plasma Cells From Patients With Multiple Myeloma. Bone Marrow Transplant. 1997;19(6):553-6. PubMed PMID: 9085734.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of the hematopoietic stem cell antigen CD34 on blood and bone marrow monoclonal plasma cells from patients with multiple myeloma. AU - Kimlinger,T, AU - Witzig,T E, PY - 1997/3/2/pubmed PY - 1997/3/2/medline PY - 1997/3/2/entrez SP - 553 EP - 6 JF - Bone marrow transplantation JO - Bone Marrow Transplant VL - 19 IS - 6 N2 - Monoclonal plasma cells (CD38+CD45-/dim) are typically present in the blood of patients with active myeloma and can contaminate stem cell harvests. This has led to strategies that select CD34+ cells for use in autologous stem cell transplantation with the goal of decreasing tumor cell contamination. The aim of this study was to learn if the CD34 antigen is expressed on monoclonal plasma cells in the blood or marrow of patients with multiple myeloma. We used three-color flow cytometry (surface CD38;CD45 and cytoplasmic kappa or lambda) to identify monoclonal plasma cells in the blood (n = 24) and marrow (n = 37) from patients with plasma cell proliferative disorders. In each case the CD38+CD45- and CD38+CD45dim+ monoclonal populations were then analyzed for CD34 expression. In all 24 blood and 37 marrow samples, the CD38+CD45-monoclonal plasma cells were negative for CD34 expression. CD38+CD45dim+ monoclonal cells were documented in the blood of 11 patients and in the marrow of 33 patients and this cell population was also CD34-negative in all cases. These results indicate that CD34 is usually not expressed on the CD38+CD45-CD45dim+ monoclonal plasma cells in the blood or marrow of patients with plasma cell proliferative disorders. CD34 selection methods should therefore decrease the chance of tumor cell contamination of the stem cell product. SN - 0268-3369 UR - https://www.unboundmedicine.com/medline/citation/9085734/Expression_of_the_hematopoietic_stem_cell_antigen_CD34_on_blood_and_bone_marrow_monoclonal_plasma_cells_from_patients_with_multiple_myeloma_ L2 - https://doi.org/10.1038/sj.bmt.1700697 DB - PRIME DP - Unbound Medicine ER -