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Reduced Gs protein function and G alpha s levels in leukocytes of patients with Parkinson's disease.
Mov Disord. 1997 Mar; 12(2):167-74.MD

Abstract

Early events in signal information transduction beyond dopamine, beta-adrenergic, and muscarinic receptors, involving receptor-coupled G-protein function and G alpha subunit immunoreactive levels were measured in mononuclear leukocytes (MNLs) of 12 never-treated patients with Parkinson's disease in comparison with 10 age- and sex-matched healthy control subjects. Both beta-adrenergic and dopamine receptor-coupled Gs protein function as measured by cholera toxin-sensitive, isoproterenol- and dopamine-induced increases in Gpp(NH)p-binding capacity, in MNLs of patients with Parkinson's disease were found to be significantly reduced in comparison with those in the control group. Muscarinic receptor-coupled non-Gs (Gi or G(o)) protein function: pertussis toxin-sensitive, carbamylcholine-induced increase in Gpp(NH)p-binding capacity, was not found to be significantly different between patients with Parkinson's disease and control subjects. G protein alpha subunits were measured through immunoblotting analyses with specific polyclonal antibodies against G alpha s, G alpha i, and G alpha q subunits. MNL levels of the 45-kDa species of G alpha s were found to be significantly reduced in patients with Parkinson's disease in comparison with control subjects. Other non-Gs proteins (Gi, Gq) did not show any significant quantitative differences between patients with Parkinson's disease and control subjects. The reductions in G alpha s levels in MNLs of patients with Parkinson's disease may explain the beta-adrenergic and dopamine receptor-coupled Gs protein hypofunction detected in MNLs of these patients. As previous studies have failed to observe significant changes in receptor levels in MNLs of patients with Parkinson's disease, our findings of reduced dopaminergic and beta-adrenergic receptor-coupled Gs function and of G alpha s immunoreactive levels in MNLs of Parkinson's patients point to alterations distal to these receptors at the level of the signal-transducing Gs protein.

Authors+Show Affiliations

Department of Clinical Pharmacology, Faculty for Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9087974

Citation

Avissar, S, et al. "Reduced Gs Protein Function and G Alpha S Levels in Leukocytes of Patients With Parkinson's Disease." Movement Disorders : Official Journal of the Movement Disorder Society, vol. 12, no. 2, 1997, pp. 167-74.
Avissar S, Barki-Harrington L, Herishanu Y, et al. Reduced Gs protein function and G alpha s levels in leukocytes of patients with Parkinson's disease. Mov Disord. 1997;12(2):167-74.
Avissar, S., Barki-Harrington, L., Herishanu, Y., & Schreiber, G. (1997). Reduced Gs protein function and G alpha s levels in leukocytes of patients with Parkinson's disease. Movement Disorders : Official Journal of the Movement Disorder Society, 12(2), 167-74.
Avissar S, et al. Reduced Gs Protein Function and G Alpha S Levels in Leukocytes of Patients With Parkinson's Disease. Mov Disord. 1997;12(2):167-74. PubMed PMID: 9087974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reduced Gs protein function and G alpha s levels in leukocytes of patients with Parkinson's disease. AU - Avissar,S, AU - Barki-Harrington,L, AU - Herishanu,Y, AU - Schreiber,G, PY - 1997/3/1/pubmed PY - 1997/3/1/medline PY - 1997/3/1/entrez SP - 167 EP - 74 JF - Movement disorders : official journal of the Movement Disorder Society JO - Mov Disord VL - 12 IS - 2 N2 - Early events in signal information transduction beyond dopamine, beta-adrenergic, and muscarinic receptors, involving receptor-coupled G-protein function and G alpha subunit immunoreactive levels were measured in mononuclear leukocytes (MNLs) of 12 never-treated patients with Parkinson's disease in comparison with 10 age- and sex-matched healthy control subjects. Both beta-adrenergic and dopamine receptor-coupled Gs protein function as measured by cholera toxin-sensitive, isoproterenol- and dopamine-induced increases in Gpp(NH)p-binding capacity, in MNLs of patients with Parkinson's disease were found to be significantly reduced in comparison with those in the control group. Muscarinic receptor-coupled non-Gs (Gi or G(o)) protein function: pertussis toxin-sensitive, carbamylcholine-induced increase in Gpp(NH)p-binding capacity, was not found to be significantly different between patients with Parkinson's disease and control subjects. G protein alpha subunits were measured through immunoblotting analyses with specific polyclonal antibodies against G alpha s, G alpha i, and G alpha q subunits. MNL levels of the 45-kDa species of G alpha s were found to be significantly reduced in patients with Parkinson's disease in comparison with control subjects. Other non-Gs proteins (Gi, Gq) did not show any significant quantitative differences between patients with Parkinson's disease and control subjects. The reductions in G alpha s levels in MNLs of patients with Parkinson's disease may explain the beta-adrenergic and dopamine receptor-coupled Gs protein hypofunction detected in MNLs of these patients. As previous studies have failed to observe significant changes in receptor levels in MNLs of patients with Parkinson's disease, our findings of reduced dopaminergic and beta-adrenergic receptor-coupled Gs function and of G alpha s immunoreactive levels in MNLs of Parkinson's patients point to alterations distal to these receptors at the level of the signal-transducing Gs protein. SN - 0885-3185 UR - https://www.unboundmedicine.com/medline/citation/9087974/Reduced_Gs_protein_function_and_G_alpha_s_levels_in_leukocytes_of_patients_with_Parkinson's_disease_ L2 - https://doi.org/10.1002/mds.870120206 DB - PRIME DP - Unbound Medicine ER -