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The broad spectrum of cytokine gene expression by myoid cells from the human marrow microenvironment.
Stem Cells. 1997; 15(2):133-43.SC

Abstract

Nontransformed stromal colony-derived cell lines (CDCLs) consist of a pure stromal cell population that differentiates following a vascular smooth muscle cell repertoire, and whose in vivo counterpart is that of myoid cells found in adult and fetal human bone marrow cords. We studied the cytokine expression by reverse-transcriptase polymerase chain reaction (RT-PCR) from pooled fast-growing clones from 10 different bone marrow samples. RT-PCR indicated that 30 cytokines (out of 42 studied) were expressed by CDCLs (20 after medium renewal and hydrocortisone renewal, three after addition of interleukin I beta (IL-1 beta) and seven in only part of the CDCL layers examined). The cytokines expressed comprised mediators known to be involved in the maintenance of early and late hematopoiesis (IL-1 alpha and IL-beta, IL-6, IL-7, IL-8, IL-11 and IL-13; colony-stimulating factors, thrombopoietin, erythropoietin, stem cell factor, fit 3-ligand, hepatocyte cell growth factor, tumor necrosis factor alpha, leukemia inhibitory factor, transforming growth factors beta 1 and beta 3; and macrophage inflammatory protein 1 alpha), angiogenic factors (fibroblast growth factors 1 and 2, vascular endothelial growth factor) and mediators whose usual target (and source) is the connective tissue-forming cells (platelet-derived growth factor A, epidermal growth factor, transforming growth factors alpha and beta 2, oncostatin M and insulin-like growth factor 1), or neuronal cells (nerve growth factor). The cytokines not expressed were lymphokines (IL-2, IL-3, IL-4, IL-5, IL-9, IL-10, and IL-12 and interferon gamma) or mediators synthesized by macrophages (inhibin, activin, platelet-derived growth factor B, and IL-1 receptor antagonist). This study complements the description of the phenotype of the myoid cells, confirming that these cells are the marrow connective tissue-forming cells; moreover, this work suggests that stromal control of hematopoiesis is multifactorial and that myoid cells are involved in the control of marrow angiogenesis and innervation.

Authors+Show Affiliations

Sensebe L
Laboratoire d'Etude de l'Hématopoièse, Etablissement de Transfusion Sanguine, Besançon, France.
Deschaseaux M
No affiliation info available
Li J
No affiliation info available
Herve P
No affiliation info available
Charbord P
No affiliation info available

MeSH

Blotting, WesternBone MarrowBone Marrow CellsCell LineCytokinesFlow CytometryFluorescent Antibody Technique, IndirectGene ExpressionHumansInterleukin-1PhenotypePolymerase Chain ReactionStromal CellsVimentin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9090790

Citation

Sensebe, L, et al. "The Broad Spectrum of Cytokine Gene Expression By Myoid Cells From the Human Marrow Microenvironment." Stem Cells (Dayton, Ohio), vol. 15, no. 2, 1997, pp. 133-43.
Sensebe L, Deschaseaux M, Li J, et al. The broad spectrum of cytokine gene expression by myoid cells from the human marrow microenvironment. Stem Cells. 1997;15(2):133-43.
Sensebe, L., Deschaseaux, M., Li, J., Herve, P., & Charbord, P. (1997). The broad spectrum of cytokine gene expression by myoid cells from the human marrow microenvironment. Stem Cells (Dayton, Ohio), 15(2), 133-43.
Sensebe L, et al. The Broad Spectrum of Cytokine Gene Expression By Myoid Cells From the Human Marrow Microenvironment. Stem Cells. 1997;15(2):133-43. PubMed PMID: 9090790.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The broad spectrum of cytokine gene expression by myoid cells from the human marrow microenvironment. AU - Sensebe,L, AU - Deschaseaux,M, AU - Li,J, AU - Herve,P, AU - Charbord,P, PY - 1997/1/1/pubmed PY - 1997/1/1/medline PY - 1997/1/1/entrez SP - 133 EP - 43 JF - Stem cells (Dayton, Ohio) JO - Stem Cells VL - 15 IS - 2 N2 - Nontransformed stromal colony-derived cell lines (CDCLs) consist of a pure stromal cell population that differentiates following a vascular smooth muscle cell repertoire, and whose in vivo counterpart is that of myoid cells found in adult and fetal human bone marrow cords. We studied the cytokine expression by reverse-transcriptase polymerase chain reaction (RT-PCR) from pooled fast-growing clones from 10 different bone marrow samples. RT-PCR indicated that 30 cytokines (out of 42 studied) were expressed by CDCLs (20 after medium renewal and hydrocortisone renewal, three after addition of interleukin I beta (IL-1 beta) and seven in only part of the CDCL layers examined). The cytokines expressed comprised mediators known to be involved in the maintenance of early and late hematopoiesis (IL-1 alpha and IL-beta, IL-6, IL-7, IL-8, IL-11 and IL-13; colony-stimulating factors, thrombopoietin, erythropoietin, stem cell factor, fit 3-ligand, hepatocyte cell growth factor, tumor necrosis factor alpha, leukemia inhibitory factor, transforming growth factors beta 1 and beta 3; and macrophage inflammatory protein 1 alpha), angiogenic factors (fibroblast growth factors 1 and 2, vascular endothelial growth factor) and mediators whose usual target (and source) is the connective tissue-forming cells (platelet-derived growth factor A, epidermal growth factor, transforming growth factors alpha and beta 2, oncostatin M and insulin-like growth factor 1), or neuronal cells (nerve growth factor). The cytokines not expressed were lymphokines (IL-2, IL-3, IL-4, IL-5, IL-9, IL-10, and IL-12 and interferon gamma) or mediators synthesized by macrophages (inhibin, activin, platelet-derived growth factor B, and IL-1 receptor antagonist). This study complements the description of the phenotype of the myoid cells, confirming that these cells are the marrow connective tissue-forming cells; moreover, this work suggests that stromal control of hematopoiesis is multifactorial and that myoid cells are involved in the control of marrow angiogenesis and innervation. SN - 1066-5099 UR - https://www.unboundmedicine.com/medline/citation/9090790/The_broad_spectrum_of_cytokine_gene_expression_by_myoid_cells_from_the_human_marrow_microenvironment_ L2 - https://doi.org/10.1002/stem.150133 DB - PRIME DP - Unbound Medicine ER -