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Relationship between the mucosal production of reactive oxygen radicals and density of Helicobacter pylori in patients with duodenal ulcer.
Eur J Gastroenterol Hepatol. 1997 Mar; 9(3):261-5.EJ

Abstract

OBJECTIVE

To investigate the associations between the mucosal production of reactive oxygen radicals (RORs) in the gastric antrum and duodenum, Helicobacter pylori density and duodenal ulcer (DU).

PATIENTS

Forty-seven endoscoped patients, comprising 22 with DU and 25 non-ulcer subjects, were included in the study.

METHODS

Antral and duodenal biopsies were taken for histology, Helicobacter pylori culture and measurement of chemiluminescence. Biopsies were homogenized and cultured on Columbia blood agar plate. Colonies of H. pylori were counted and bacterial density expressed as colony-forming units (cfu)/mg of biopsy. Chemiluminescence was measured by luminometry and the results expressed as millivolt (mV)/min/mg of biopsy, after subtraction of background count.

RESULTS

Thirty-one of 47 (66%) patients had antral H. pylori and 6/47 (12.8%) had proven duodenal colonization. Increased chemiluminescence (median (interquartile)) was found in H. pylori-infected patients compared to those without H. pylori in antral (90.0 (26.0, 249.0) vs. 7.0 (0.0, 10.0), P<0.001) and duodenal mucosa (22.0 (10.0, 100.0) vs. -2.5 (-10.0, 0.0) P<0.001). A positive correlation was found between antral H. pylori density and chemiluminescence response in both the antrum (r=0.77) and duodenum (r=0.52). DU patients showed an increased chemiluminescence compared to those non-ulcer subjects with or without H. pylori infection in antrum (163.5 (44.5, 297.8) vs. 33.0 (8.7, 168.0) (P=0.046) vs. 2.7 (0.1, 10.0), P<0.01) and duodenum (45.0 (17.5, 100.0) vs. 15.0 (-1.25, 22.5) vs. -2.5 (-10, 0.0), P<0.01).

CONCLUSION

Increased production of RORs in the antrum and duodenum was found to be related to antral H. pylori density and associated with duodenal ulceration. The association between antral H. pylori and ROR release in the duodenum may be important in the pathogenesis of duodenal ulceration.

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Authors+Show Affiliations

Zhang Q
Department of Gastroenterology, Royal Infirmary, Glasgow, UK.
Dawodu JB
No affiliation info available
Etolhi G
No affiliation info available
Husain A
No affiliation info available
Gemmell CG
No affiliation info available
Russell RI
No affiliation info available

MeSH

BiopsyCytotoxinsDuodenal UlcerDuodenumGastric MucosaHelicobacter pyloriHumansIntestinal MucosaNeutrophilsPyloric AntrumReactive Oxygen Species

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9096427

Citation

Zhang, Q, et al. "Relationship Between the Mucosal Production of Reactive Oxygen Radicals and Density of Helicobacter Pylori in Patients With Duodenal Ulcer." European Journal of Gastroenterology & Hepatology, vol. 9, no. 3, 1997, pp. 261-5.
Zhang Q, Dawodu JB, Etolhi G, et al. Relationship between the mucosal production of reactive oxygen radicals and density of Helicobacter pylori in patients with duodenal ulcer. Eur J Gastroenterol Hepatol. 1997;9(3):261-5.
Zhang, Q., Dawodu, J. B., Etolhi, G., Husain, A., Gemmell, C. G., & Russell, R. I. (1997). Relationship between the mucosal production of reactive oxygen radicals and density of Helicobacter pylori in patients with duodenal ulcer. European Journal of Gastroenterology & Hepatology, 9(3), 261-5.
Zhang Q, et al. Relationship Between the Mucosal Production of Reactive Oxygen Radicals and Density of Helicobacter Pylori in Patients With Duodenal Ulcer. Eur J Gastroenterol Hepatol. 1997;9(3):261-5. PubMed PMID: 9096427.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relationship between the mucosal production of reactive oxygen radicals and density of Helicobacter pylori in patients with duodenal ulcer. AU - Zhang,Q, AU - Dawodu,J B, AU - Etolhi,G, AU - Husain,A, AU - Gemmell,C G, AU - Russell,R I, PY - 1997/3/1/pubmed PY - 1997/3/1/medline PY - 1997/3/1/entrez SP - 261 EP - 5 JF - European journal of gastroenterology & hepatology JO - Eur J Gastroenterol Hepatol VL - 9 IS - 3 N2 - OBJECTIVE: To investigate the associations between the mucosal production of reactive oxygen radicals (RORs) in the gastric antrum and duodenum, Helicobacter pylori density and duodenal ulcer (DU). PATIENTS: Forty-seven endoscoped patients, comprising 22 with DU and 25 non-ulcer subjects, were included in the study. METHODS: Antral and duodenal biopsies were taken for histology, Helicobacter pylori culture and measurement of chemiluminescence. Biopsies were homogenized and cultured on Columbia blood agar plate. Colonies of H. pylori were counted and bacterial density expressed as colony-forming units (cfu)/mg of biopsy. Chemiluminescence was measured by luminometry and the results expressed as millivolt (mV)/min/mg of biopsy, after subtraction of background count. RESULTS: Thirty-one of 47 (66%) patients had antral H. pylori and 6/47 (12.8%) had proven duodenal colonization. Increased chemiluminescence (median (interquartile)) was found in H. pylori-infected patients compared to those without H. pylori in antral (90.0 (26.0, 249.0) vs. 7.0 (0.0, 10.0), P<0.001) and duodenal mucosa (22.0 (10.0, 100.0) vs. -2.5 (-10.0, 0.0) P<0.001). A positive correlation was found between antral H. pylori density and chemiluminescence response in both the antrum (r=0.77) and duodenum (r=0.52). DU patients showed an increased chemiluminescence compared to those non-ulcer subjects with or without H. pylori infection in antrum (163.5 (44.5, 297.8) vs. 33.0 (8.7, 168.0) (P=0.046) vs. 2.7 (0.1, 10.0), P<0.01) and duodenum (45.0 (17.5, 100.0) vs. 15.0 (-1.25, 22.5) vs. -2.5 (-10, 0.0), P<0.01). CONCLUSION: Increased production of RORs in the antrum and duodenum was found to be related to antral H. pylori density and associated with duodenal ulceration. The association between antral H. pylori and ROR release in the duodenum may be important in the pathogenesis of duodenal ulceration. SN - 0954-691X UR - https://www.unboundmedicine.com/medline/citation/9096427/Relationship_between_the_mucosal_production_of_reactive_oxygen_radicals_and_density_of_Helicobacter_pylori_in_patients_with_duodenal_ulcer_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&amp;PAGE=linkout&amp;SEARCH=9096427.ui DB - PRIME DP - Unbound Medicine ER -