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In vitro cultivation of Gymnophalloides seoi metacercariae (Digenea:Gymnophallidae).
Korean J Parasitol. 1997 Mar; 35(1):25-9.KJ

Abstract

Gymnophalloides seoi is a human intestinal trematode prevalent on southwestern islands in Korea. In the present study, we investigated whether G. seoi metacercariae can grow and develop into adults by in vitro cultivation. The metacercariae were obtained from naturally infected oysters, and cultured in vitro for 5 days under three conditions; 37 degrees C/5% CO2, 41 degrees C/8% CO2, or 41 degrees C/5% CO2, in NCTC 109 complete media containing 20% FBS and 1% antibiotics-antimycotics. The degree of worm growth and development was compared with that grown in vivo of C3H mice. The length of the worms cultivated in vitro was 200-300 microns not significantly different from metacercariae, whereas the length of the worms recovered from C3H mice was significantly larger, 300-400 microns. The worms produced eggs when grown in C3H mice or cultured in vitro for 2 days under 41 degrees C/8% CO2 or 41 degrees C/5% CO2, but not when cultured under 37 degrees C/5% CO2. Among the in vitro conditions, 41 degrees C/5% CO2 was best for egg production, although the number of eggs was about half of worms obtained from C3H mice. In conclusion, in vitro cultivation of G. seoi metacercariae into egg-producing adults was partially successful under culture conditions of 41 degrees C/5% CO2 or 41 degrees C/8% CO2.

Authors+Show Affiliations

Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Korea.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9100436

Citation

Kook, J, et al. "In Vitro Cultivation of Gymnophalloides Seoi Metacercariae (Digenea:Gymnophallidae)." The Korean Journal of Parasitology, vol. 35, no. 1, 1997, pp. 25-9.
Kook J, Lee SH, Chai JY. In vitro cultivation of Gymnophalloides seoi metacercariae (Digenea:Gymnophallidae). Korean J Parasitol. 1997;35(1):25-9.
Kook, J., Lee, S. H., & Chai, J. Y. (1997). In vitro cultivation of Gymnophalloides seoi metacercariae (Digenea:Gymnophallidae). The Korean Journal of Parasitology, 35(1), 25-9.
Kook J, Lee SH, Chai JY. In Vitro Cultivation of Gymnophalloides Seoi Metacercariae (Digenea:Gymnophallidae). Korean J Parasitol. 1997;35(1):25-9. PubMed PMID: 9100436.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro cultivation of Gymnophalloides seoi metacercariae (Digenea:Gymnophallidae). AU - Kook,J, AU - Lee,S H, AU - Chai,J Y, PY - 1997/3/1/pubmed PY - 1997/3/1/medline PY - 1997/3/1/entrez SP - 25 EP - 9 JF - The Korean journal of parasitology JO - Korean J. Parasitol. VL - 35 IS - 1 N2 - Gymnophalloides seoi is a human intestinal trematode prevalent on southwestern islands in Korea. In the present study, we investigated whether G. seoi metacercariae can grow and develop into adults by in vitro cultivation. The metacercariae were obtained from naturally infected oysters, and cultured in vitro for 5 days under three conditions; 37 degrees C/5% CO2, 41 degrees C/8% CO2, or 41 degrees C/5% CO2, in NCTC 109 complete media containing 20% FBS and 1% antibiotics-antimycotics. The degree of worm growth and development was compared with that grown in vivo of C3H mice. The length of the worms cultivated in vitro was 200-300 microns not significantly different from metacercariae, whereas the length of the worms recovered from C3H mice was significantly larger, 300-400 microns. The worms produced eggs when grown in C3H mice or cultured in vitro for 2 days under 41 degrees C/8% CO2 or 41 degrees C/5% CO2, but not when cultured under 37 degrees C/5% CO2. Among the in vitro conditions, 41 degrees C/5% CO2 was best for egg production, although the number of eggs was about half of worms obtained from C3H mice. In conclusion, in vitro cultivation of G. seoi metacercariae into egg-producing adults was partially successful under culture conditions of 41 degrees C/5% CO2 or 41 degrees C/8% CO2. SN - 0023-4001 UR - https://www.unboundmedicine.com/medline/citation/9100436/In_vitro_cultivation_of_Gymnophalloides_seoi_metacercariae__Digenea:Gymnophallidae__ L2 - http://www.parasitol.or.kr/kjp/DOIx.php?id=10.3347/kjp.1997.35.1.25 DB - PRIME DP - Unbound Medicine ER -