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Capillary zone electrophoresis in skin fatty acid analysis.
Pharmazie. 1997 Mar; 52(3):212-5.P

Abstract

The development of a method for analyzing C12-C18 fatty acid alkali salts by capillary zone electrophoresis is described. Organic solvents were added to the aqueous separation buffer to achieve solubility of the fatty acids together with higher temperatures. Detection of these poorly UV absorbing species could be achieved by indirect photometric detection adding a highly UV absorbing co-ion to the separation buffer. Thus detection limits of 6 microM were recorded. The dynamic range of quantitation was limited to 10(1) due to association and micelle formation at higher concentrations. The relative standard deviation of the method was in the range of 4 to 10%. The described method could be successfully applied to the analysis of free fatty acids in human stratum corneum after solid phase extraction of the lipid fraction.

Authors+Show Affiliations

Neubert R
Institute for Pharmaceutics and Biopharmaceutics, Martin-Luther-University Halle-Wittenberg, Germany.
Raith K
No affiliation info available
Schiewe J
No affiliation info available

MeSH

CalibrationElectrophoresis, CapillaryFatty AcidsFatty Acids, NonesterifiedHumansSkinSpectrophotometry, Ultraviolet

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9109168

Citation

Neubert, R, et al. "Capillary Zone Electrophoresis in Skin Fatty Acid Analysis." Die Pharmazie, vol. 52, no. 3, 1997, pp. 212-5.
Neubert R, Raith K, Schiewe J. Capillary zone electrophoresis in skin fatty acid analysis. Pharmazie. 1997;52(3):212-5.
Neubert, R., Raith, K., & Schiewe, J. (1997). Capillary zone electrophoresis in skin fatty acid analysis. Die Pharmazie, 52(3), 212-5.
Neubert R, Raith K, Schiewe J. Capillary Zone Electrophoresis in Skin Fatty Acid Analysis. Pharmazie. 1997;52(3):212-5. PubMed PMID: 9109168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary zone electrophoresis in skin fatty acid analysis. AU - Neubert,R, AU - Raith,K, AU - Schiewe,J, PY - 1997/3/1/pubmed PY - 1997/3/1/medline PY - 1997/3/1/entrez SP - 212 EP - 5 JF - Die Pharmazie JO - Pharmazie VL - 52 IS - 3 N2 - The development of a method for analyzing C12-C18 fatty acid alkali salts by capillary zone electrophoresis is described. Organic solvents were added to the aqueous separation buffer to achieve solubility of the fatty acids together with higher temperatures. Detection of these poorly UV absorbing species could be achieved by indirect photometric detection adding a highly UV absorbing co-ion to the separation buffer. Thus detection limits of 6 microM were recorded. The dynamic range of quantitation was limited to 10(1) due to association and micelle formation at higher concentrations. The relative standard deviation of the method was in the range of 4 to 10%. The described method could be successfully applied to the analysis of free fatty acids in human stratum corneum after solid phase extraction of the lipid fraction. SN - 0031-7144 UR - https://www.unboundmedicine.com/medline/citation/9109168/Capillary_zone_electrophoresis_in_skin_fatty_acid_analysis_ DB - PRIME DP - Unbound Medicine ER -