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Proliferation kinetics and PCNA expression of HL-60 cells following ionizing irradiation and granulocytic differentiation.
Cell Prolif 1993; 26(6):531-43CP

Abstract

The human promyelocytic leukaemia cell line, HL-60, was investigated with regard to proliferation and terminal differentiation following irradiation. The cells were X-irradiated and induced with 1.25% dimethyl sulfoxide (DMSO) towards the granulocytic lineage. Proliferation was measured via cell growth, clonogenicity and the bromodeoxyuridine/DNA incorporation assay. Immunohistochemical detection of proliferating cell nuclear antigen (PCNA) expression was used to discriminate cycling from non-cycling cells. The differentiation obtained was proved by testing for the immune function of the respiratory burst (NBT reduction test). The HL-60 cells studied revealed a high radiosensitivity (D0 = 0.63 Gy). After induction with DMSO, declines in cell growth, clonogenicity and PCNA positivity of the cells indicated a decrease in proliferation and an increase in differentiation. Starting on day 2 in culture, irradiation after seeding with 1 Gy accelerated the loss of the PCNA expression in induced cells (46% v. 3% PCNA-negative control cells on day 3). Induced cells gained the capability of exerting the respiratory burst, which was found to be dose-dependent radiosensitive (42%, and 12% NBT-positive cells after 1 and 2 Gy, respectively, v. 53% NBT-positive control cells on day 8). Subpopulations in the cell line were evident in all parameters investigated. We discuss the HL-60 cell, not only as a model comparable to human progenitor cells, but also as a suitable tool in radiobiological research with regard to proliferation and differentiation following ionizing irradiation.

Authors+Show Affiliations

Institute of Radiobiology, Federal Armed Forces Medical Academy, Munich, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9116120

Citation

Sendler, A, et al. "Proliferation Kinetics and PCNA Expression of HL-60 Cells Following Ionizing Irradiation and Granulocytic Differentiation." Cell Proliferation, vol. 26, no. 6, 1993, pp. 531-43.
Sendler A, Kaffenberger W, Nuyken I, et al. Proliferation kinetics and PCNA expression of HL-60 cells following ionizing irradiation and granulocytic differentiation. Cell Prolif. 1993;26(6):531-43.
Sendler, A., Kaffenberger, W., Nuyken, I., & van Beuningen, D. (1993). Proliferation kinetics and PCNA expression of HL-60 cells following ionizing irradiation and granulocytic differentiation. Cell Proliferation, 26(6), pp. 531-43.
Sendler A, et al. Proliferation Kinetics and PCNA Expression of HL-60 Cells Following Ionizing Irradiation and Granulocytic Differentiation. Cell Prolif. 1993;26(6):531-43. PubMed PMID: 9116120.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proliferation kinetics and PCNA expression of HL-60 cells following ionizing irradiation and granulocytic differentiation. AU - Sendler,A, AU - Kaffenberger,W, AU - Nuyken,I, AU - van Beuningen,D, PY - 1993/11/1/pubmed PY - 1993/11/1/medline PY - 1993/11/1/entrez SP - 531 EP - 43 JF - Cell proliferation JO - Cell Prolif. VL - 26 IS - 6 N2 - The human promyelocytic leukaemia cell line, HL-60, was investigated with regard to proliferation and terminal differentiation following irradiation. The cells were X-irradiated and induced with 1.25% dimethyl sulfoxide (DMSO) towards the granulocytic lineage. Proliferation was measured via cell growth, clonogenicity and the bromodeoxyuridine/DNA incorporation assay. Immunohistochemical detection of proliferating cell nuclear antigen (PCNA) expression was used to discriminate cycling from non-cycling cells. The differentiation obtained was proved by testing for the immune function of the respiratory burst (NBT reduction test). The HL-60 cells studied revealed a high radiosensitivity (D0 = 0.63 Gy). After induction with DMSO, declines in cell growth, clonogenicity and PCNA positivity of the cells indicated a decrease in proliferation and an increase in differentiation. Starting on day 2 in culture, irradiation after seeding with 1 Gy accelerated the loss of the PCNA expression in induced cells (46% v. 3% PCNA-negative control cells on day 3). Induced cells gained the capability of exerting the respiratory burst, which was found to be dose-dependent radiosensitive (42%, and 12% NBT-positive cells after 1 and 2 Gy, respectively, v. 53% NBT-positive control cells on day 8). Subpopulations in the cell line were evident in all parameters investigated. We discuss the HL-60 cell, not only as a model comparable to human progenitor cells, but also as a suitable tool in radiobiological research with regard to proliferation and differentiation following ionizing irradiation. SN - 0960-7722 UR - https://www.unboundmedicine.com/medline/citation/9116120/Proliferation_kinetics_and_PCNA_expression_of_HL-60_cells_following_ionizing_irradiation_and_granulocytic_differentiation L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0960-7722&date=1993&volume=26&issue=6&spage=531 DB - PRIME DP - Unbound Medicine ER -