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Trans-activation of group II intron splicing by nuclear U5 snRNA.
Nature. 1997 Mar 27; 386(6623):417-20.Nat

Abstract

Similarities between RNA splicing during autocatalytic excision of group II introns and pre-mRNA processing led to the hypothesis that group II introns might be the evolutionary predecessors of spliceosomal small nuclear RNAs. The ID3 subdomain stem-loop structure of group II introns, the proposed analogue of the spliceosomal U5 snRNA, is thought to be essential for 5' splice site recognition and anchoring of the free 5' exon. Using the group II intron bI1 we have analysed the role of ID3 in splicing. In the absence of ID3 the 5' splice site was recognized accurately and efficiently, but exon anchoring was greatly reduced. This step was restored in the presence of RNA fragments consisting of either the terminal stem-loop structure of ID3 or spliceosomal U5 snRNA. This suggests that the predominant role of both RNAs is to anchor the 5' exon during exon ligation. Furthermore, as U5 complements for the loss of ID3, a similar network of structural RNAs may form the catalytic core of both group II introns and spliceosomes.

Authors+Show Affiliations

Vienna Biocenter, Institute of Microbiology and Genetics, University of Vienna, Austria. hetzer@gem.univie.ac.atNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9121561

Citation

Hetzer, M, et al. "Trans-activation of Group II Intron Splicing By Nuclear U5 SnRNA." Nature, vol. 386, no. 6623, 1997, pp. 417-20.
Hetzer M, Wurzer G, Schweyen RJ, et al. Trans-activation of group II intron splicing by nuclear U5 snRNA. Nature. 1997;386(6623):417-20.
Hetzer, M., Wurzer, G., Schweyen, R. J., & Mueller, M. W. (1997). Trans-activation of group II intron splicing by nuclear U5 snRNA. Nature, 386(6623), 417-20.
Hetzer M, et al. Trans-activation of Group II Intron Splicing By Nuclear U5 SnRNA. Nature. 1997 Mar 27;386(6623):417-20. PubMed PMID: 9121561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Trans-activation of group II intron splicing by nuclear U5 snRNA. AU - Hetzer,M, AU - Wurzer,G, AU - Schweyen,R J, AU - Mueller,M W, PY - 1997/3/27/pubmed PY - 1997/3/27/medline PY - 1997/3/27/entrez SP - 417 EP - 20 JF - Nature JO - Nature VL - 386 IS - 6623 N2 - Similarities between RNA splicing during autocatalytic excision of group II introns and pre-mRNA processing led to the hypothesis that group II introns might be the evolutionary predecessors of spliceosomal small nuclear RNAs. The ID3 subdomain stem-loop structure of group II introns, the proposed analogue of the spliceosomal U5 snRNA, is thought to be essential for 5' splice site recognition and anchoring of the free 5' exon. Using the group II intron bI1 we have analysed the role of ID3 in splicing. In the absence of ID3 the 5' splice site was recognized accurately and efficiently, but exon anchoring was greatly reduced. This step was restored in the presence of RNA fragments consisting of either the terminal stem-loop structure of ID3 or spliceosomal U5 snRNA. This suggests that the predominant role of both RNAs is to anchor the 5' exon during exon ligation. Furthermore, as U5 complements for the loss of ID3, a similar network of structural RNAs may form the catalytic core of both group II introns and spliceosomes. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/9121561/Trans_activation_of_group_II_intron_splicing_by_nuclear_U5_snRNA_ L2 - https://doi.org/10.1038/386417a0 DB - PRIME DP - Unbound Medicine ER -