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Presentation of abundant endogenous class II DR-restricted antigens by DM-negative B cell lines.
Eur J Immunol. 1997 Apr; 27(4):1014-21.EJ

Abstract

Peptides derived from endogenous and exogenous antigens compete for binding and presentation via class II molecules. Studies with mutant B cell lines defective in exogenous antigen presentation suggest that HLA-DM molecules facilitate the interaction of foreign peptides and class II molecules. In contrast, presentation of self antigens is not strictly dependent upon HLA-DM, as demonstrated by the ability of these mutant cells to activate T cells specific for endogenous antigens. Two distinct classes of DM-negative cells, T2 cells generated by in vitro mutagenesis and lines derived from bare lymphocyte syndrome (BLS) patients, were able to present epitopes derived from self proteins. Transfection of DM genes into the mutant cells enhanced the presentation of some, but not all, endogenous antigens, suggesting that formation of select endogenous peptide/class II complexes is not dependent upon DM. The efficiency of endogenous antigen presentation in the absence of DM was also dependent on the mutant antigen-presenting cell studied, as the TxB hybrid T2 presented greater amounts of self peptides compared to cells from BLS patients. Thus, additional genes, aside from DM, may regulate the pathway for endogenous antigen presentation.

Authors+Show Affiliations

Department of Immunology, University of Washington School of Medicine, Seattle 98195, USA. skovats@u.washington.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9130658

Citation

Kovats, S, et al. "Presentation of Abundant Endogenous Class II DR-restricted Antigens By DM-negative B Cell Lines." European Journal of Immunology, vol. 27, no. 4, 1997, pp. 1014-21.
Kovats S, Whiteley PE, Concannon P, et al. Presentation of abundant endogenous class II DR-restricted antigens by DM-negative B cell lines. Eur J Immunol. 1997;27(4):1014-21.
Kovats, S., Whiteley, P. E., Concannon, P., Rudensky, A. Y., & Blum, J. S. (1997). Presentation of abundant endogenous class II DR-restricted antigens by DM-negative B cell lines. European Journal of Immunology, 27(4), 1014-21.
Kovats S, et al. Presentation of Abundant Endogenous Class II DR-restricted Antigens By DM-negative B Cell Lines. Eur J Immunol. 1997;27(4):1014-21. PubMed PMID: 9130658.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Presentation of abundant endogenous class II DR-restricted antigens by DM-negative B cell lines. AU - Kovats,S, AU - Whiteley,P E, AU - Concannon,P, AU - Rudensky,A Y, AU - Blum,J S, PY - 1997/4/1/pubmed PY - 1997/4/1/medline PY - 1997/4/1/entrez SP - 1014 EP - 21 JF - European journal of immunology JO - Eur J Immunol VL - 27 IS - 4 N2 - Peptides derived from endogenous and exogenous antigens compete for binding and presentation via class II molecules. Studies with mutant B cell lines defective in exogenous antigen presentation suggest that HLA-DM molecules facilitate the interaction of foreign peptides and class II molecules. In contrast, presentation of self antigens is not strictly dependent upon HLA-DM, as demonstrated by the ability of these mutant cells to activate T cells specific for endogenous antigens. Two distinct classes of DM-negative cells, T2 cells generated by in vitro mutagenesis and lines derived from bare lymphocyte syndrome (BLS) patients, were able to present epitopes derived from self proteins. Transfection of DM genes into the mutant cells enhanced the presentation of some, but not all, endogenous antigens, suggesting that formation of select endogenous peptide/class II complexes is not dependent upon DM. The efficiency of endogenous antigen presentation in the absence of DM was also dependent on the mutant antigen-presenting cell studied, as the TxB hybrid T2 presented greater amounts of self peptides compared to cells from BLS patients. Thus, additional genes, aside from DM, may regulate the pathway for endogenous antigen presentation. SN - 0014-2980 UR - https://www.unboundmedicine.com/medline/citation/9130658/Presentation_of_abundant_endogenous_class_II_DR_restricted_antigens_by_DM_negative_B_cell_lines_ L2 - https://doi.org/10.1002/eji.1830270431 DB - PRIME DP - Unbound Medicine ER -