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Diagnostic value of a new sensitive membrane based technique for instantaneous D-dimer evaluation in patients with clinically suspected deep venous thrombosis.
Thromb Haemost. 1997 Apr; 77(4):637-40.TH

Abstract

BACKGROUND

Plasma D-Dimer analysis, using ELISA assays, has demonstrated in previous studies a high sensitivity, suggesting its utility in excluding deep venous thrombosis (DVT).

AIM

To assess the performance of a new rapid plasma D-Dimer ELISA measurement in suspected DVT patients with recent clinical signs, not exceeding one week.

METHODS

A prospective study of patients admitted for a suspected recent DVT. Contrast venography or compression ultrasonography were performed within 24 h of admission. A new membrane based ELISA technique, which uses an immunofiltration and two complementary monoclonal antibodies was tested. Results were expressed as positive or negative. A standard plasma D-Dimer ELISA measurement was also performed. D-Dimer performances were assessed at the end of the study.

RESULTS

265/448 patients had a proven DVT (72 distal, 193 proximal). The sensitivity of the instantaneous method in the diagnosis of overall DVT is 92 +/- 3.4% (95% CI), and specificity is 36.6 +/- 6.9%. Positive predictive value is 67.7 +/- 4.8% and negative predictive value is 76.1 +/- 8.9%. Sensitivity and negative predictive values reach 97.9 and 94.3% in the diagnosis of proximal DVT, but only 76.3 and 79.7% in the diagnosis of distal DVT. Similar results are observed with the standard ELISA method.

CONCLUSION

This new rapid plasma D-Dimer measurement appears highly sensitive, and could substitute the older ELISA methods. Both methods provide lower sensitivity in the case of a distal DVT location.

Authors+Show Affiliations

Leroyer C
Department of Chest Diseases and Internal Medicine, Hôpital de la Cavale Blanche, Brest, France.
Escoffre M
No affiliation info available
Le Moigne E
No affiliation info available
Grimaux M
No affiliation info available
Cagnioncle O
No affiliation info available
Oger E
No affiliation info available
Bressollette L
No affiliation info available
Abgrall JF
No affiliation info available
Amiral J
No affiliation info available
Mottier D
No affiliation info available

MeSH

AdolescentAdultAgedAged, 80 and overEnzyme-Linked Immunosorbent AssayEvaluation Studies as TopicFemaleFibrin Fibrinogen Degradation ProductsHumansMaleMembranes, ArtificialMiddle AgedPhlebographyPredictive Value of TestsProspective StudiesReagent Kits, DiagnosticSensitivity and SpecificityThrombophlebitisTime FactorsUltrasonography

Pub Type(s)

Clinical Trial
Controlled Clinical Trial
Journal Article

Language

eng

PubMed ID

9134634

Citation

Leroyer, C, et al. "Diagnostic Value of a New Sensitive Membrane Based Technique for Instantaneous D-dimer Evaluation in Patients With Clinically Suspected Deep Venous Thrombosis." Thrombosis and Haemostasis, vol. 77, no. 4, 1997, pp. 637-40.
Leroyer C, Escoffre M, Le Moigne E, et al. Diagnostic value of a new sensitive membrane based technique for instantaneous D-dimer evaluation in patients with clinically suspected deep venous thrombosis. Thromb Haemost. 1997;77(4):637-40.
Leroyer, C., Escoffre, M., Le Moigne, E., Grimaux, M., Cagnioncle, O., Oger, E., Bressollette, L., Abgrall, J. F., Amiral, J., & Mottier, D. (1997). Diagnostic value of a new sensitive membrane based technique for instantaneous D-dimer evaluation in patients with clinically suspected deep venous thrombosis. Thrombosis and Haemostasis, 77(4), 637-40.
Leroyer C, et al. Diagnostic Value of a New Sensitive Membrane Based Technique for Instantaneous D-dimer Evaluation in Patients With Clinically Suspected Deep Venous Thrombosis. Thromb Haemost. 1997;77(4):637-40. PubMed PMID: 9134634.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnostic value of a new sensitive membrane based technique for instantaneous D-dimer evaluation in patients with clinically suspected deep venous thrombosis. AU - Leroyer,C, AU - Escoffre,M, AU - Le Moigne,E, AU - Grimaux,M, AU - Cagnioncle,O, AU - Oger,E, AU - Bressollette,L, AU - Abgrall,J F, AU - Amiral,J, AU - Mottier,D, PY - 1997/4/1/pubmed PY - 1997/4/1/medline PY - 1997/4/1/entrez SP - 637 EP - 40 JF - Thrombosis and haemostasis JO - Thromb Haemost VL - 77 IS - 4 N2 - BACKGROUND: Plasma D-Dimer analysis, using ELISA assays, has demonstrated in previous studies a high sensitivity, suggesting its utility in excluding deep venous thrombosis (DVT). AIM: To assess the performance of a new rapid plasma D-Dimer ELISA measurement in suspected DVT patients with recent clinical signs, not exceeding one week. METHODS: A prospective study of patients admitted for a suspected recent DVT. Contrast venography or compression ultrasonography were performed within 24 h of admission. A new membrane based ELISA technique, which uses an immunofiltration and two complementary monoclonal antibodies was tested. Results were expressed as positive or negative. A standard plasma D-Dimer ELISA measurement was also performed. D-Dimer performances were assessed at the end of the study. RESULTS: 265/448 patients had a proven DVT (72 distal, 193 proximal). The sensitivity of the instantaneous method in the diagnosis of overall DVT is 92 +/- 3.4% (95% CI), and specificity is 36.6 +/- 6.9%. Positive predictive value is 67.7 +/- 4.8% and negative predictive value is 76.1 +/- 8.9%. Sensitivity and negative predictive values reach 97.9 and 94.3% in the diagnosis of proximal DVT, but only 76.3 and 79.7% in the diagnosis of distal DVT. Similar results are observed with the standard ELISA method. CONCLUSION: This new rapid plasma D-Dimer measurement appears highly sensitive, and could substitute the older ELISA methods. Both methods provide lower sensitivity in the case of a distal DVT location. SN - 0340-6245 UR - https://www.unboundmedicine.com/medline/citation/9134634/Diagnostic_value_of_a_new_sensitive_membrane_based_technique_for_instantaneous_D_dimer_evaluation_in_patients_with_clinically_suspected_deep_venous_thrombosis_ L2 - http://www.diseaseinfosearch.org/result/7087 DB - PRIME DP - Unbound Medicine ER -