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Position-independent and high-level expression of human alpha-lactalbumin in the milk of transgenic rats carrying a 210-kb YAC DNA.
Mol Reprod Dev. 1997 Jun; 47(2):157-63.MR

Abstract

The level of expression of transgenes in transgenic animals varies among lines, and is often much lower than that of endogenous genes (position effects). In order to surmount position effects and establish a more efficient production system of transgenic animals producing pharmaceutical proteins in their milk, transgenic rats carrying 210-kb YAC DNA containing the human alpha-lactalbumin gene were produced. Three transgenic lines transmitted the transgene to the next generation. They had one copy of the alpha-lactalbumin gene and secreted human alpha-lactalbumin in their milk at concentrations of 2.0-4.3 mg/ml. No position effect was seen. The transgene was expressed specifically in the mammary gland of the transgenic rats. The 210-kb region is thought to contain all the DNA elements required for proper expression of the human alpha-lactalbumin gene. The YAC carrying the human alpha-lactalbumin gene is a potential vector for the expression of foreign genes in the mammary gland.

Authors+Show Affiliations

YS New Technology Institute, Inc., Tochigi, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9136116

Citation

Fujiwara, Y, et al. "Position-independent and High-level Expression of Human Alpha-lactalbumin in the Milk of Transgenic Rats Carrying a 210-kb YAC DNA." Molecular Reproduction and Development, vol. 47, no. 2, 1997, pp. 157-63.
Fujiwara Y, Miwa M, Takahashi R, et al. Position-independent and high-level expression of human alpha-lactalbumin in the milk of transgenic rats carrying a 210-kb YAC DNA. Mol Reprod Dev. 1997;47(2):157-63.
Fujiwara, Y., Miwa, M., Takahashi, R., Hirabayashi, M., Suzuki, T., & Ueda, M. (1997). Position-independent and high-level expression of human alpha-lactalbumin in the milk of transgenic rats carrying a 210-kb YAC DNA. Molecular Reproduction and Development, 47(2), 157-63.
Fujiwara Y, et al. Position-independent and High-level Expression of Human Alpha-lactalbumin in the Milk of Transgenic Rats Carrying a 210-kb YAC DNA. Mol Reprod Dev. 1997;47(2):157-63. PubMed PMID: 9136116.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Position-independent and high-level expression of human alpha-lactalbumin in the milk of transgenic rats carrying a 210-kb YAC DNA. AU - Fujiwara,Y, AU - Miwa,M, AU - Takahashi,R, AU - Hirabayashi,M, AU - Suzuki,T, AU - Ueda,M, PY - 1997/6/1/pubmed PY - 2000/6/20/medline PY - 1997/6/1/entrez SP - 157 EP - 63 JF - Molecular reproduction and development JO - Mol. Reprod. Dev. VL - 47 IS - 2 N2 - The level of expression of transgenes in transgenic animals varies among lines, and is often much lower than that of endogenous genes (position effects). In order to surmount position effects and establish a more efficient production system of transgenic animals producing pharmaceutical proteins in their milk, transgenic rats carrying 210-kb YAC DNA containing the human alpha-lactalbumin gene were produced. Three transgenic lines transmitted the transgene to the next generation. They had one copy of the alpha-lactalbumin gene and secreted human alpha-lactalbumin in their milk at concentrations of 2.0-4.3 mg/ml. No position effect was seen. The transgene was expressed specifically in the mammary gland of the transgenic rats. The 210-kb region is thought to contain all the DNA elements required for proper expression of the human alpha-lactalbumin gene. The YAC carrying the human alpha-lactalbumin gene is a potential vector for the expression of foreign genes in the mammary gland. SN - 1040-452X UR - https://www.unboundmedicine.com/medline/citation/9136116/Position_independent_and_high_level_expression_of_human_alpha_lactalbumin_in_the_milk_of_transgenic_rats_carrying_a_210_kb_YAC_DNA_ L2 - https://doi.org/10.1002/(SICI)1098-2795(199706)47:2<157::AID-MRD5>3.0.CO;2-L DB - PRIME DP - Unbound Medicine ER -