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Inhibitor (IK) of IFN-gamma induced HLA class II antigens expression also inhibits HLA class II constitutive expression in the human Raji B cell line.
Oncogene. 1997 Mar 27; 14(12):1453-61.O

Abstract

The expression of major histocompatibility complex (MHC) class II antigens is constitutive in professional antigen presenting cells (APCs) but can also be induced by interferon-gamma (IFN-gamma) on the majority of the non professional APCs (e.g. fibroblasts). We have recently characterised a new factor called IK which is an efficient inhibitor of IFN-gamma induction of MHC class II antigens expression. Here, we demonstrate a novel role for IK in MHC class II expression since over-expression of this protein by stable transfection into human B cells led to a total disappearance of constitutive MHC class II mRNA expression. The class II transactivator (CIITA) is necessary for both constitutive and IFN-gamma induced MHC class II expressions. Examination of CIITA mRNA in IK stably transfected clones revealed a marked reduction of CIITA mRNA transcription. Taken together these results demonstrate that the IK protein plays a key role in the constitutive expression of MHC class II antigens and that inhibition induced by IK is upstream of CIITA in this regulatory pathway.

Authors+Show Affiliations

INSERM U 396, Institut Biomédical des Cordeliers, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9136989

Citation

Vedrenne, J, et al. "Inhibitor (IK) of IFN-gamma Induced HLA Class II Antigens Expression Also Inhibits HLA Class II Constitutive Expression in the Human Raji B Cell Line." Oncogene, vol. 14, no. 12, 1997, pp. 1453-61.
Vedrenne J, Assier E, Pereno R, et al. Inhibitor (IK) of IFN-gamma induced HLA class II antigens expression also inhibits HLA class II constitutive expression in the human Raji B cell line. Oncogene. 1997;14(12):1453-61.
Vedrenne, J., Assier, E., Pereno, R., Bouzinba-Segard, H., Azzarone, B., Jasmin, C., Charron, D., & Krief, P. (1997). Inhibitor (IK) of IFN-gamma induced HLA class II antigens expression also inhibits HLA class II constitutive expression in the human Raji B cell line. Oncogene, 14(12), 1453-61.
Vedrenne J, et al. Inhibitor (IK) of IFN-gamma Induced HLA Class II Antigens Expression Also Inhibits HLA Class II Constitutive Expression in the Human Raji B Cell Line. Oncogene. 1997 Mar 27;14(12):1453-61. PubMed PMID: 9136989.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitor (IK) of IFN-gamma induced HLA class II antigens expression also inhibits HLA class II constitutive expression in the human Raji B cell line. AU - Vedrenne,J, AU - Assier,E, AU - Pereno,R, AU - Bouzinba-Segard,H, AU - Azzarone,B, AU - Jasmin,C, AU - Charron,D, AU - Krief,P, PY - 1997/3/27/pubmed PY - 1997/3/27/medline PY - 1997/3/27/entrez SP - 1453 EP - 61 JF - Oncogene JO - Oncogene VL - 14 IS - 12 N2 - The expression of major histocompatibility complex (MHC) class II antigens is constitutive in professional antigen presenting cells (APCs) but can also be induced by interferon-gamma (IFN-gamma) on the majority of the non professional APCs (e.g. fibroblasts). We have recently characterised a new factor called IK which is an efficient inhibitor of IFN-gamma induction of MHC class II antigens expression. Here, we demonstrate a novel role for IK in MHC class II expression since over-expression of this protein by stable transfection into human B cells led to a total disappearance of constitutive MHC class II mRNA expression. The class II transactivator (CIITA) is necessary for both constitutive and IFN-gamma induced MHC class II expressions. Examination of CIITA mRNA in IK stably transfected clones revealed a marked reduction of CIITA mRNA transcription. Taken together these results demonstrate that the IK protein plays a key role in the constitutive expression of MHC class II antigens and that inhibition induced by IK is upstream of CIITA in this regulatory pathway. SN - 0950-9232 UR - https://www.unboundmedicine.com/medline/citation/9136989/Inhibitor__IK__of_IFN_gamma_induced_HLA_class_II_antigens_expression_also_inhibits_HLA_class_II_constitutive_expression_in_the_human_Raji_B_cell_line_ L2 - https://doi.org/10.1038/sj.onc.1200971 DB - PRIME DP - Unbound Medicine ER -