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In vitro and in vivo expression of inducible nitric oxide synthase during experimental endotoxemia: involvement of other cytokines.
J Cell Biochem. 1997 Jun 01; 65(3):349-58.JC

Abstract

In this study, we investigated the expression of genes for inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNF-alpha), interleukin 1 beta (IL-1 beta), interleukin 6 (IL-6) of Kupffer cells in the presence of lipopolysaccharide (LPS), and the tissue expression of iNOS in a rat liver after LPS injection at various time intervals. The effects of L-NG-nitroarginine-methyl-esther HCI (L-NAME), a NO inhibitor, also were examined. The mRNA transcripts of TNF-alpha, IL-1 beta, and IL-6 were rapidly detected no more than at 1 h after LPS stimulation, whereas the iNOS transcript was detectable from 3 h after LPS stimulation and maximally increased at 12 h. This fact suggested that these early induced cytokines were related to expression of iNOS. Using an anti-iNOS antiserum raised against recombinant iNOS protein, immunohistochemical analysis was made to reveal kinetics of NO producing cells. The cells immunoreactive for iNOS appeared at 6 h post-LPS injection in the sinusoids of the liver. By structural and immunohistochemical studies, almost all iNOS positive cells were identified as Kupffer cells and endothelial cells. The number of cells immunoreactive for iNOS increased until 12 h post-LPS injection. At 24 h after LPS injection, iNOS positive cells were restricted to the foci of spotty necrosis. Hepatic injury measured by released enzymes was increased by pretreatment of L-NAME before LPS injection.

Authors+Show Affiliations

First Department of Surgery, Nagoya University School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9138091

Citation

Aono, K, et al. "In Vitro and in Vivo Expression of Inducible Nitric Oxide Synthase During Experimental Endotoxemia: Involvement of Other Cytokines." Journal of Cellular Biochemistry, vol. 65, no. 3, 1997, pp. 349-58.
Aono K, Isobe K, Kiuchi K, et al. In vitro and in vivo expression of inducible nitric oxide synthase during experimental endotoxemia: involvement of other cytokines. J Cell Biochem. 1997;65(3):349-58.
Aono, K., Isobe, K., Kiuchi, K., Fan, Z. H., Ito, M., Takeuchi, A., Miyachi, M., Nakashima, I., & Nimura, Y. (1997). In vitro and in vivo expression of inducible nitric oxide synthase during experimental endotoxemia: involvement of other cytokines. Journal of Cellular Biochemistry, 65(3), 349-58.
Aono K, et al. In Vitro and in Vivo Expression of Inducible Nitric Oxide Synthase During Experimental Endotoxemia: Involvement of Other Cytokines. J Cell Biochem. 1997 Jun 1;65(3):349-58. PubMed PMID: 9138091.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro and in vivo expression of inducible nitric oxide synthase during experimental endotoxemia: involvement of other cytokines. AU - Aono,K, AU - Isobe,K, AU - Kiuchi,K, AU - Fan,Z H, AU - Ito,M, AU - Takeuchi,A, AU - Miyachi,M, AU - Nakashima,I, AU - Nimura,Y, PY - 1997/6/1/pubmed PY - 2000/6/20/medline PY - 1997/6/1/entrez SP - 349 EP - 58 JF - Journal of cellular biochemistry JO - J Cell Biochem VL - 65 IS - 3 N2 - In this study, we investigated the expression of genes for inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNF-alpha), interleukin 1 beta (IL-1 beta), interleukin 6 (IL-6) of Kupffer cells in the presence of lipopolysaccharide (LPS), and the tissue expression of iNOS in a rat liver after LPS injection at various time intervals. The effects of L-NG-nitroarginine-methyl-esther HCI (L-NAME), a NO inhibitor, also were examined. The mRNA transcripts of TNF-alpha, IL-1 beta, and IL-6 were rapidly detected no more than at 1 h after LPS stimulation, whereas the iNOS transcript was detectable from 3 h after LPS stimulation and maximally increased at 12 h. This fact suggested that these early induced cytokines were related to expression of iNOS. Using an anti-iNOS antiserum raised against recombinant iNOS protein, immunohistochemical analysis was made to reveal kinetics of NO producing cells. The cells immunoreactive for iNOS appeared at 6 h post-LPS injection in the sinusoids of the liver. By structural and immunohistochemical studies, almost all iNOS positive cells were identified as Kupffer cells and endothelial cells. The number of cells immunoreactive for iNOS increased until 12 h post-LPS injection. At 24 h after LPS injection, iNOS positive cells were restricted to the foci of spotty necrosis. Hepatic injury measured by released enzymes was increased by pretreatment of L-NAME before LPS injection. SN - 0730-2312 UR - https://www.unboundmedicine.com/medline/citation/9138091/In_vitro_and_in_vivo_expression_of_inducible_nitric_oxide_synthase_during_experimental_endotoxemia:_involvement_of_other_cytokines_ L2 - https://antibodies.cancer.gov/detail/CPTC-IL6-1 DB - PRIME DP - Unbound Medicine ER -