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Detection of circulating antigens of Parastrongylus cantonensis in human sera by sandwich ELISA with specific monoclonal antibody.

Abstract

A specific monoclonal antibody (AW-3C2) as revealed by ELISA was produced against the adult worm antigens of Parastrongylus cantonensis and used in a sandwich ELISA for the detection of circulating antigens in the sera of parastrongyliasis patients and those with other parasitic diseases. A total of 60 sera was used in this study. Of these, 10 each were from patients with parastrongyliasis, cysticercosis, filariasis, gnathostomiasis, malaria and toxocariasis. The control group consisted of 53 serum samples from normal healthy Thais and Malaysians. The mean +/- optical density (OD) values for the normal Thai and Malaysian groups were 0.126 +/- 0.028 and 0.124 +/- 0.029, respectively. The mean OD values of the parastrongyliasis patient group differed significantly from that of the normal groups as well as those of other parasitic infections. Using a cut-off point of OD +/- 3SD of the control groups as indicating a positive reading, the specificity of the assay with this monoclonal antibody was 100% while the sensitivity was 50%.

Authors+Show Affiliations

Department of Medical Sciences, Ministry of Public Health, Bangkok, Malaysia.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9139382

Citation

Eamsobhana, P, et al. "Detection of Circulating Antigens of Parastrongylus Cantonensis in Human Sera By Sandwich ELISA With Specific Monoclonal Antibody." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 26, no. 4, 1995, pp. 712-5.
Eamsobhana P, Mak JW, Yong HS. Detection of circulating antigens of Parastrongylus cantonensis in human sera by sandwich ELISA with specific monoclonal antibody. Southeast Asian J Trop Med Public Health. 1995;26(4):712-5.
Eamsobhana, P., Mak, J. W., & Yong, H. S. (1995). Detection of circulating antigens of Parastrongylus cantonensis in human sera by sandwich ELISA with specific monoclonal antibody. The Southeast Asian Journal of Tropical Medicine and Public Health, 26(4), 712-5.
Eamsobhana P, Mak JW, Yong HS. Detection of Circulating Antigens of Parastrongylus Cantonensis in Human Sera By Sandwich ELISA With Specific Monoclonal Antibody. Southeast Asian J Trop Med Public Health. 1995;26(4):712-5. PubMed PMID: 9139382.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of circulating antigens of Parastrongylus cantonensis in human sera by sandwich ELISA with specific monoclonal antibody. AU - Eamsobhana,P, AU - Mak,J W, AU - Yong,H S, PY - 1995/12/1/pubmed PY - 1995/12/1/medline PY - 1995/12/1/entrez SP - 712 EP - 5 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 26 IS - 4 N2 - A specific monoclonal antibody (AW-3C2) as revealed by ELISA was produced against the adult worm antigens of Parastrongylus cantonensis and used in a sandwich ELISA for the detection of circulating antigens in the sera of parastrongyliasis patients and those with other parasitic diseases. A total of 60 sera was used in this study. Of these, 10 each were from patients with parastrongyliasis, cysticercosis, filariasis, gnathostomiasis, malaria and toxocariasis. The control group consisted of 53 serum samples from normal healthy Thais and Malaysians. The mean +/- optical density (OD) values for the normal Thai and Malaysian groups were 0.126 +/- 0.028 and 0.124 +/- 0.029, respectively. The mean OD values of the parastrongyliasis patient group differed significantly from that of the normal groups as well as those of other parasitic infections. Using a cut-off point of OD +/- 3SD of the control groups as indicating a positive reading, the specificity of the assay with this monoclonal antibody was 100% while the sensitivity was 50%. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/9139382/Detection_of_circulating_antigens_of_Parastrongylus_cantonensis_in_human_sera_by_sandwich_ELISA_with_specific_monoclonal_antibody_ DB - PRIME DP - Unbound Medicine ER -