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Molecular characterization of the Aspergillus nidulans treA gene encoding an acid trehalase required for growth on trehalose.
Mol Microbiol. 1997 Apr; 24(1):203-16.MM

Abstract

Aspergillus nidulans conidiospores contain high levels of the non-reducing disaccharide trehalose. We show that upon induction of conidiospore germination, the trehalose pool is rapidly degraded and a glycerol pool is transiently accumulated. A trehalase with an acidic pH optimum was purified from conidiospores. Characterization of the treA gene encoding this trehalase shows that it is homologous to Saccharomyces cerevisiae vacuolar acid trehalase, the product of the ATH1 gene, and to two related proteins of unknown function identified in Mycobacterium tuberculosis and Mycobacterium leprae. A. nidulans mutants that lack acid trehalase activity were constructed by gene replacement at the treA locus. Analysis of these mutants suggests that the treA gene product is localized in the conidiospore wall, is required for growth on trehalose as a carbon source, and is not involved in the mobilization of the intracellular pool of trehalose. Therefore, it is proposed that a cytoplasmic regulatory trehalase is controlling this latter process.

Authors+Show Affiliations

Laboratoire des Aspergillus, Institut Pasteur, Paris, France. denfert@pasteur.frNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9140977

Citation

d'Enfert, C, and T Fontaine. "Molecular Characterization of the Aspergillus Nidulans treA Gene Encoding an Acid Trehalase Required for Growth On Trehalose." Molecular Microbiology, vol. 24, no. 1, 1997, pp. 203-16.
d'Enfert C, Fontaine T. Molecular characterization of the Aspergillus nidulans treA gene encoding an acid trehalase required for growth on trehalose. Mol Microbiol. 1997;24(1):203-16.
d'Enfert, C., & Fontaine, T. (1997). Molecular characterization of the Aspergillus nidulans treA gene encoding an acid trehalase required for growth on trehalose. Molecular Microbiology, 24(1), 203-16.
d'Enfert C, Fontaine T. Molecular Characterization of the Aspergillus Nidulans treA Gene Encoding an Acid Trehalase Required for Growth On Trehalose. Mol Microbiol. 1997;24(1):203-16. PubMed PMID: 9140977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of the Aspergillus nidulans treA gene encoding an acid trehalase required for growth on trehalose. AU - d'Enfert,C, AU - Fontaine,T, PY - 1997/4/1/pubmed PY - 1997/4/1/medline PY - 1997/4/1/entrez SP - 203 EP - 16 JF - Molecular microbiology JO - Mol Microbiol VL - 24 IS - 1 N2 - Aspergillus nidulans conidiospores contain high levels of the non-reducing disaccharide trehalose. We show that upon induction of conidiospore germination, the trehalose pool is rapidly degraded and a glycerol pool is transiently accumulated. A trehalase with an acidic pH optimum was purified from conidiospores. Characterization of the treA gene encoding this trehalase shows that it is homologous to Saccharomyces cerevisiae vacuolar acid trehalase, the product of the ATH1 gene, and to two related proteins of unknown function identified in Mycobacterium tuberculosis and Mycobacterium leprae. A. nidulans mutants that lack acid trehalase activity were constructed by gene replacement at the treA locus. Analysis of these mutants suggests that the treA gene product is localized in the conidiospore wall, is required for growth on trehalose as a carbon source, and is not involved in the mobilization of the intracellular pool of trehalose. Therefore, it is proposed that a cytoplasmic regulatory trehalase is controlling this latter process. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/9140977/Molecular_characterization_of_the_Aspergillus_nidulans_treA_gene_encoding_an_acid_trehalase_required_for_growth_on_trehalose_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=1997&volume=24&issue=1&spage=203 DB - PRIME DP - Unbound Medicine ER -