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Use of an excision reporter plasmid to study the intracellular mobility of the conjugative transposon Tn916 in gram-positive bacteria.
Microbiology 1997; 143 (Pt 4):1253-61M

Abstract

An excision reporter plasmid was constructed to characterize the intracellular mobility of Tn916 in various Gram-positive bacteria. The reporter component of this plasmid is a chloramphenicol-resistance gene which has been insertionally inactivated with the integrative vector pAT112 containing the attachment site of Tn916. Tn916-mediated excision of pAT112, to produce clones resistant to chloramphenicol, was detected in Enterococcus faecalis BM4110, Listeria monocytogenes L028-Str and Streptococcus gordonii BM120, but not in Lactococcus lactis MG1363-RF or in Streptococcus pneumoniae BM124, and always depended upon the ability of the bacterial host to generate circular forms of the transposon. The results suggest that (i) the excision event, although required, is not sufficient for conjugal transfer to occur and (ii) there is no linear relationship between the donor potential of E. faecalis strains and either the excision frequency of pAT112 or the copy number of Tn916 circular intermediates per cell in these hosts. Excision of pAT112 occurred mainly during the late exponential phase of growth of E. faecalis and L. monocytogenes and this recombination event was not stimulated by heat shock, salt and alcohol stresses or by the presence of tetracycline in the medium.

Authors+Show Affiliations

Laboratoire de Microbiologie, Faculté de Médecine Necker-Enfants Malades, Paris, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9141688

Citation

Celli, J, et al. "Use of an Excision Reporter Plasmid to Study the Intracellular Mobility of the Conjugative Transposon Tn916 in Gram-positive Bacteria." Microbiology (Reading, England), vol. 143 (Pt 4), 1997, pp. 1253-61.
Celli J, Poyart C, Trieu-Cuot P. Use of an excision reporter plasmid to study the intracellular mobility of the conjugative transposon Tn916 in gram-positive bacteria. Microbiology (Reading, Engl). 1997;143 (Pt 4):1253-61.
Celli, J., Poyart, C., & Trieu-Cuot, P. (1997). Use of an excision reporter plasmid to study the intracellular mobility of the conjugative transposon Tn916 in gram-positive bacteria. Microbiology (Reading, England), 143 (Pt 4), pp. 1253-61.
Celli J, Poyart C, Trieu-Cuot P. Use of an Excision Reporter Plasmid to Study the Intracellular Mobility of the Conjugative Transposon Tn916 in Gram-positive Bacteria. Microbiology (Reading, Engl). 1997;143 (Pt 4):1253-61. PubMed PMID: 9141688.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of an excision reporter plasmid to study the intracellular mobility of the conjugative transposon Tn916 in gram-positive bacteria. AU - Celli,J, AU - Poyart,C, AU - Trieu-Cuot,P, PY - 1997/4/1/pubmed PY - 1997/4/1/medline PY - 1997/4/1/entrez SP - 1253 EP - 61 JF - Microbiology (Reading, England) JO - Microbiology (Reading, Engl.) VL - 143 (Pt 4) N2 - An excision reporter plasmid was constructed to characterize the intracellular mobility of Tn916 in various Gram-positive bacteria. The reporter component of this plasmid is a chloramphenicol-resistance gene which has been insertionally inactivated with the integrative vector pAT112 containing the attachment site of Tn916. Tn916-mediated excision of pAT112, to produce clones resistant to chloramphenicol, was detected in Enterococcus faecalis BM4110, Listeria monocytogenes L028-Str and Streptococcus gordonii BM120, but not in Lactococcus lactis MG1363-RF or in Streptococcus pneumoniae BM124, and always depended upon the ability of the bacterial host to generate circular forms of the transposon. The results suggest that (i) the excision event, although required, is not sufficient for conjugal transfer to occur and (ii) there is no linear relationship between the donor potential of E. faecalis strains and either the excision frequency of pAT112 or the copy number of Tn916 circular intermediates per cell in these hosts. Excision of pAT112 occurred mainly during the late exponential phase of growth of E. faecalis and L. monocytogenes and this recombination event was not stimulated by heat shock, salt and alcohol stresses or by the presence of tetracycline in the medium. SN - 1350-0872 UR - https://www.unboundmedicine.com/medline/citation/9141688/Use_of_an_excision_reporter_plasmid_to_study_the_intracellular_mobility_of_the_conjugative_transposon_Tn916_in_gram_positive_bacteria_ L2 - http://mic.microbiologyresearch.org/pubmed/content/journal/micro/10.1099/00221287-143-4-1253 DB - PRIME DP - Unbound Medicine ER -