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Diagnosis of infections caused by Enterocytozoon bieneusi and Encephalitozoon intestinalis using polymerase chain reaction in stool specimens.
AIDS. 1997 May; 11(6):723-6.AIDS

Abstract

OBJECTIVE

To study the usefulness of polymerase chain reaction (PCR) for the species identification of microsporidia in stool specimens obtained from HIV-infected patients with Enterocytozoon bieneusi or Encephalitozoon intestinalis infections.

SETTING

Infectious disease clinic in a university hospital.

PATIENTS

Thirty-seven stool specimens from 29 HIV-infected patients with microsporidiosis were tested. The diagnosis of microsporidian infection was made by light microscopy of stool specimens and species identification was made by transmission electron microscopy of duodenal biopsies. Sixty-one stool specimens from 45 HIV-infected patients without microsporidiosis served as controls.

METHODS

PCR was performed using DNA extracted from stools with two primers sets, one specific for E. bieneusi and one specific for E. intestinalis.

RESULTS

A 1265 base-pair fragment of the small subunit ribosomal RNA (rrs) gene could be amplified from all 31 stool specimens infected with E. bieneusi. In addition, a 930 base-pair fragment of the rrs gene could be amplified from all six stool specimens infected with E. intestinalis. The 61 control stools were negative with both primers.

CONCLUSIONS

These results suggest that a PCR based assay using species-specific primers sets can be used successfully for microsporidian species differentiation from stool specimens, thus obviating the need for invasive biopsy procedures.

Authors+Show Affiliations

Division of Infectious Diseases, Hôpital Saint-Louis, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9143603

Citation

Liguory, O, et al. "Diagnosis of Infections Caused By Enterocytozoon Bieneusi and Encephalitozoon Intestinalis Using Polymerase Chain Reaction in Stool Specimens." AIDS (London, England), vol. 11, no. 6, 1997, pp. 723-6.
Liguory O, David F, Sarfati C, et al. Diagnosis of infections caused by Enterocytozoon bieneusi and Encephalitozoon intestinalis using polymerase chain reaction in stool specimens. AIDS. 1997;11(6):723-6.
Liguory, O., David, F., Sarfati, C., Schuitema, A. R., Hartskeerl, R. A., Derouin, F., Modaï, J., & Molina, J. M. (1997). Diagnosis of infections caused by Enterocytozoon bieneusi and Encephalitozoon intestinalis using polymerase chain reaction in stool specimens. AIDS (London, England), 11(6), 723-6.
Liguory O, et al. Diagnosis of Infections Caused By Enterocytozoon Bieneusi and Encephalitozoon Intestinalis Using Polymerase Chain Reaction in Stool Specimens. AIDS. 1997;11(6):723-6. PubMed PMID: 9143603.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis of infections caused by Enterocytozoon bieneusi and Encephalitozoon intestinalis using polymerase chain reaction in stool specimens. AU - Liguory,O, AU - David,F, AU - Sarfati,C, AU - Schuitema,A R, AU - Hartskeerl,R A, AU - Derouin,F, AU - Modaï,J, AU - Molina,J M, PY - 1997/5/1/pubmed PY - 1997/5/1/medline PY - 1997/5/1/entrez SP - 723 EP - 6 JF - AIDS (London, England) JO - AIDS VL - 11 IS - 6 N2 - OBJECTIVE: To study the usefulness of polymerase chain reaction (PCR) for the species identification of microsporidia in stool specimens obtained from HIV-infected patients with Enterocytozoon bieneusi or Encephalitozoon intestinalis infections. SETTING: Infectious disease clinic in a university hospital. PATIENTS: Thirty-seven stool specimens from 29 HIV-infected patients with microsporidiosis were tested. The diagnosis of microsporidian infection was made by light microscopy of stool specimens and species identification was made by transmission electron microscopy of duodenal biopsies. Sixty-one stool specimens from 45 HIV-infected patients without microsporidiosis served as controls. METHODS: PCR was performed using DNA extracted from stools with two primers sets, one specific for E. bieneusi and one specific for E. intestinalis. RESULTS: A 1265 base-pair fragment of the small subunit ribosomal RNA (rrs) gene could be amplified from all 31 stool specimens infected with E. bieneusi. In addition, a 930 base-pair fragment of the rrs gene could be amplified from all six stool specimens infected with E. intestinalis. The 61 control stools were negative with both primers. CONCLUSIONS: These results suggest that a PCR based assay using species-specific primers sets can be used successfully for microsporidian species differentiation from stool specimens, thus obviating the need for invasive biopsy procedures. SN - 0269-9370 UR - https://www.unboundmedicine.com/medline/citation/9143603/Diagnosis_of_infections_caused_by_Enterocytozoon_bieneusi_and_Encephalitozoon_intestinalis_using_polymerase_chain_reaction_in_stool_specimens_ L2 - https://doi.org/10.1097/00002030-199706000-00004 DB - PRIME DP - Unbound Medicine ER -