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SecE-depleted membranes of Escherichia coli are active. SecE is not obligatorily required for the in vitro translocation of certain protein precursors.
J Biol Chem 1997; 272(21):13660-5JB

Abstract

Membrane vesicles were prepared from Escherichia coli cells in which SecE was depleted to 2% of wild-type membranes. SecE depletion had pleiotropic effects; SecD, SecF, SecG, and SecY were decreased 4-6-fold, whereas SecA was increased about 16-fold over that of wild-type membranes. These membranes were substantially active in the in vitro translocation of proOmpA, which was mediated by the SecA pathway since it was inhibited by azide. Similar substantial translocation activities were observed for proLamB and proLpp in the SecE-depleted membranes. However, the translocation of proPhoA was more severely impaired. These data indicate that SecE may enhance but is not obligatorily required for the translocation of at least certain precursors, and suggest that the effects of the SecE depletion on protein translocation may be precursor-dependent.

Authors+Show Affiliations

Department of Biology, Georgia State University, Atlanta, Georgia 30303, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9153216

Citation

Yang, Y B., et al. "SecE-depleted Membranes of Escherichia Coli Are Active. SecE Is Not Obligatorily Required for the in Vitro Translocation of Certain Protein Precursors." The Journal of Biological Chemistry, vol. 272, no. 21, 1997, pp. 13660-5.
Yang YB, Yu N, Tai PC. SecE-depleted membranes of Escherichia coli are active. SecE is not obligatorily required for the in vitro translocation of certain protein precursors. J Biol Chem. 1997;272(21):13660-5.
Yang, Y. B., Yu, N., & Tai, P. C. (1997). SecE-depleted membranes of Escherichia coli are active. SecE is not obligatorily required for the in vitro translocation of certain protein precursors. The Journal of Biological Chemistry, 272(21), pp. 13660-5.
Yang YB, Yu N, Tai PC. SecE-depleted Membranes of Escherichia Coli Are Active. SecE Is Not Obligatorily Required for the in Vitro Translocation of Certain Protein Precursors. J Biol Chem. 1997 May 23;272(21):13660-5. PubMed PMID: 9153216.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SecE-depleted membranes of Escherichia coli are active. SecE is not obligatorily required for the in vitro translocation of certain protein precursors. AU - Yang,Y B, AU - Yu,N, AU - Tai,P C, PY - 1997/5/23/pubmed PY - 1997/5/23/medline PY - 1997/5/23/entrez SP - 13660 EP - 5 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 272 IS - 21 N2 - Membrane vesicles were prepared from Escherichia coli cells in which SecE was depleted to 2% of wild-type membranes. SecE depletion had pleiotropic effects; SecD, SecF, SecG, and SecY were decreased 4-6-fold, whereas SecA was increased about 16-fold over that of wild-type membranes. These membranes were substantially active in the in vitro translocation of proOmpA, which was mediated by the SecA pathway since it was inhibited by azide. Similar substantial translocation activities were observed for proLamB and proLpp in the SecE-depleted membranes. However, the translocation of proPhoA was more severely impaired. These data indicate that SecE may enhance but is not obligatorily required for the translocation of at least certain precursors, and suggest that the effects of the SecE depletion on protein translocation may be precursor-dependent. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/9153216/SecE_depleted_membranes_of_Escherichia_coli_are_active__SecE_is_not_obligatorily_required_for_the_in_vitro_translocation_of_certain_protein_precursors_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=9153216 DB - PRIME DP - Unbound Medicine ER -