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The branched-chain amino acid permease gene of Saccharomyces cerevisiae, BAP2, encodes the high-affinity leucine permease (S1).
Yeast. 1997 Apr; 13(5):435-9.Y

Abstract

The amino acid leucine has been shown previously to be transported into a yeast cell by at least three permeases: the general amino acid permease, a high-affinity permease (S1) and a low-affinity permease (S2). We isolated the gene BAP2 as a multicopy suppressor of the YPD- phenotype of aat1leu2 yeast. BAP2 has been identified previously as encoding an amino acid permease which transports branched-chain amino acids. In order to align the genetic and biochemical studies of leucine uptake we completed a detailed kinetic analysis of yeast strains in which the BAP2 gene was disrupted and compared this to the kinetics of uptake of the parental strain. We demonstrate that BAP2 encodes the high-affinity leucine permease previously called S1.

Authors+Show Affiliations

Schreve J
Department of Biology, Hamilton College, Clinton, New York 13323, USA.
Garrett JM
No affiliation info available

MeSH

Amino Acid Transport SystemsGenes, FungalKineticsLeucineMembrane Transport ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae Proteins

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9153753

Citation

Schreve, J, and J M. Garrett. "The Branched-chain Amino Acid Permease Gene of Saccharomyces Cerevisiae, BAP2, Encodes the High-affinity Leucine Permease (S1)." Yeast (Chichester, England), vol. 13, no. 5, 1997, pp. 435-9.
Schreve J, Garrett JM. The branched-chain amino acid permease gene of Saccharomyces cerevisiae, BAP2, encodes the high-affinity leucine permease (S1). Yeast. 1997;13(5):435-9.
Schreve, J., & Garrett, J. M. (1997). The branched-chain amino acid permease gene of Saccharomyces cerevisiae, BAP2, encodes the high-affinity leucine permease (S1). Yeast (Chichester, England), 13(5), 435-9.
Schreve J, Garrett JM. The Branched-chain Amino Acid Permease Gene of Saccharomyces Cerevisiae, BAP2, Encodes the High-affinity Leucine Permease (S1). Yeast. 1997;13(5):435-9. PubMed PMID: 9153753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The branched-chain amino acid permease gene of Saccharomyces cerevisiae, BAP2, encodes the high-affinity leucine permease (S1). AU - Schreve,J, AU - Garrett,J M, PY - 1997/4/1/pubmed PY - 2000/6/20/medline PY - 1997/4/1/entrez SP - 435 EP - 9 JF - Yeast (Chichester, England) JO - Yeast VL - 13 IS - 5 N2 - The amino acid leucine has been shown previously to be transported into a yeast cell by at least three permeases: the general amino acid permease, a high-affinity permease (S1) and a low-affinity permease (S2). We isolated the gene BAP2 as a multicopy suppressor of the YPD- phenotype of aat1leu2 yeast. BAP2 has been identified previously as encoding an amino acid permease which transports branched-chain amino acids. In order to align the genetic and biochemical studies of leucine uptake we completed a detailed kinetic analysis of yeast strains in which the BAP2 gene was disrupted and compared this to the kinetics of uptake of the parental strain. We demonstrate that BAP2 encodes the high-affinity leucine permease previously called S1. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/9153753/The_branched_chain_amino_acid_permease_gene_of_Saccharomyces_cerevisiae_BAP2_encodes_the_high_affinity_leucine_permease__S1__ L2 - https://doi.org/10.1002/(SICI)1097-0061(199704)13:5<435::AID-YEA95>3.0.CO;2-T DB - PRIME DP - Unbound Medicine ER -