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Isolation of histamine-containing cells from canine fundic mucosa.
Gastroenterology. 1979 Dec; 77(6):1283-90.G

Abstract

The cells of the fundic portion of canine gastric mucosa were dispersed by collagenase digestion and separated into fractions by sequential use of velocity sedimentation in an elutriator rotor followed by a density gradient separation. There was a close correlation between histamine content and number of mast cells in the different cell fractions. The mast cells possessed characteristic dense granules, which stained metachromatically, but did not release histamine on exposure to Compound 48/80. The most highly purified fractions contained 80% mast cells and a histamine content of 2.5 pg/mast cell.

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Publisher Full Text

Authors

Soll AH
No affiliation info available
Lewin K
No affiliation info available
Beaven MA
No affiliation info available

MeSH

AnimalsCell CountCell SeparationCentrifugation, Density GradientDogsGastric MucosaHistamineHistamine ReleaseMast Cellsp-Methoxy-N-methylphenethylamine

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

91540

Citation

Soll, A H., et al. "Isolation of Histamine-containing Cells From Canine Fundic Mucosa." Gastroenterology, vol. 77, no. 6, 1979, pp. 1283-90.
Soll AH, Lewin K, Beaven MA. Isolation of histamine-containing cells from canine fundic mucosa. Gastroenterology. 1979;77(6):1283-90.
Soll, A. H., Lewin, K., & Beaven, M. A. (1979). Isolation of histamine-containing cells from canine fundic mucosa. Gastroenterology, 77(6), 1283-90.
Soll AH, Lewin K, Beaven MA. Isolation of Histamine-containing Cells From Canine Fundic Mucosa. Gastroenterology. 1979;77(6):1283-90. PubMed PMID: 91540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation of histamine-containing cells from canine fundic mucosa. AU - Soll,A H, AU - Lewin,K, AU - Beaven,M A, PY - 1979/12/1/pubmed PY - 1979/12/1/medline PY - 1979/12/1/entrez SP - 1283 EP - 90 JF - Gastroenterology JO - Gastroenterology VL - 77 IS - 6 N2 - The cells of the fundic portion of canine gastric mucosa were dispersed by collagenase digestion and separated into fractions by sequential use of velocity sedimentation in an elutriator rotor followed by a density gradient separation. There was a close correlation between histamine content and number of mast cells in the different cell fractions. The mast cells possessed characteristic dense granules, which stained metachromatically, but did not release histamine on exposure to Compound 48/80. The most highly purified fractions contained 80% mast cells and a histamine content of 2.5 pg/mast cell. SN - 0016-5085 UR - https://www.unboundmedicine.com/medline/citation/91540/Isolation_of_histamine_containing_cells_from_canine_fundic_mucosa_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016508579002377 DB - PRIME DP - Unbound Medicine ER -