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Detection of circulating antigen by monoclonal antibodies for immunodiagnosis of angiostrongyliasis.
Am J Trop Med Hyg. 1997 Apr; 56(4):408-12.AJ

Abstract

Two monoclonal antibodies, which recognize the epitope on an antigen with a molecular weight of 204 kD from the fifth-stage worm of Angiostrongylus cantonensis, were previously prepared and used to detect circulating antigens in patients with eosinophilic meningitis or meningoencephalitis and in mice experimentally infected with this parasite by a double-antibody, sandwich enzyme-linked immunosorbent assay (ELISA). The levels of this circulating antigen in experimentally infected mice were significantly higher three weeks after infection. The ELISA values in the detection of circulating antigens in cerebrospinal fluid (CSF) from patients were markedly higher than those in serum. Immunodiagnosis of patients with angiostrongyliasis by this technique proved to be highly specific for circulating antigens in serum and CSF specimens; however, the sensitivity in CSF was significantly higher than in serum.

Authors+Show Affiliations

Department of Parasitology, Kaohsiung Medical College, Taiwan, Republic of China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9158049

Citation

Chye, S M., et al. "Detection of Circulating Antigen By Monoclonal Antibodies for Immunodiagnosis of Angiostrongyliasis." The American Journal of Tropical Medicine and Hygiene, vol. 56, no. 4, 1997, pp. 408-12.
Chye SM, Yen CM, Chen ER. Detection of circulating antigen by monoclonal antibodies for immunodiagnosis of angiostrongyliasis. Am J Trop Med Hyg. 1997;56(4):408-12.
Chye, S. M., Yen, C. M., & Chen, E. R. (1997). Detection of circulating antigen by monoclonal antibodies for immunodiagnosis of angiostrongyliasis. The American Journal of Tropical Medicine and Hygiene, 56(4), 408-12.
Chye SM, Yen CM, Chen ER. Detection of Circulating Antigen By Monoclonal Antibodies for Immunodiagnosis of Angiostrongyliasis. Am J Trop Med Hyg. 1997;56(4):408-12. PubMed PMID: 9158049.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of circulating antigen by monoclonal antibodies for immunodiagnosis of angiostrongyliasis. AU - Chye,S M, AU - Yen,C M, AU - Chen,E R, PY - 1997/4/1/pubmed PY - 1997/4/1/medline PY - 1997/4/1/entrez SP - 408 EP - 12 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 56 IS - 4 N2 - Two monoclonal antibodies, which recognize the epitope on an antigen with a molecular weight of 204 kD from the fifth-stage worm of Angiostrongylus cantonensis, were previously prepared and used to detect circulating antigens in patients with eosinophilic meningitis or meningoencephalitis and in mice experimentally infected with this parasite by a double-antibody, sandwich enzyme-linked immunosorbent assay (ELISA). The levels of this circulating antigen in experimentally infected mice were significantly higher three weeks after infection. The ELISA values in the detection of circulating antigens in cerebrospinal fluid (CSF) from patients were markedly higher than those in serum. Immunodiagnosis of patients with angiostrongyliasis by this technique proved to be highly specific for circulating antigens in serum and CSF specimens; however, the sensitivity in CSF was significantly higher than in serum. SN - 0002-9637 UR - https://www.unboundmedicine.com/medline/citation/9158049/Detection_of_circulating_antigen_by_monoclonal_antibodies_for_immunodiagnosis_of_angiostrongyliasis_ L2 - https://ajtmh.org/doi/10.4269/ajtmh.1997.56.408 DB - PRIME DP - Unbound Medicine ER -