Tags

Type your tag names separated by a space and hit enter

The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide, and lipase.
Mol Microbiol. 1997 Apr; 24(2):309-19.MM

Abstract

The global activator GacA, a highly conserved response regulator in Gram-negative bacteria, is required for the production of exoenzymes and secondary metabolites in Pseudomonas spp. The gacA gene of Pseudomonas aeruginosa PAO1 was isolated and its role in cell-density-dependent gene expression was characterized. Mutational inactivation of gacA resulted in delayed and reduced formation of the cell-density signal N-butyryl-L-homoserine lactone (BHL), of the cognate transcriptional activator RhIR (VsmR), and of the transcriptional activator LasR, which is known to positively regulate RhIR expression. Amplification of gacA on a multicopy plasmid caused precocious and enhanced production of BHL, RhIR and LasR. In parallel, the gacA gene dosage markedly influenced the BHL/RhIR-dependent formation of the cytotoxic compounds pyocyanin and cyanide and the exoenzyme lipase. However, the concentrations of another known cell-density signal of P. aeruginosa, N-oxododecanoyl-L-homoserine lactone, did not always match BHL concentrations. A model accounting for these observations places GacA function upstream of LasR and RhIR in the complex, cell-density-dependent signal-transduction pathway regulating several exoproducts and virulence factors of P. aeruginosa via BHL.

Links

Publisher Full Text
Aggregator Full Text

Authors+Show Affiliations

Reimmann C
Laboratoire de Biologie Microbienne, Université de Lausanne, Switzerland.
Beyeler M
No affiliation info available
Latifi A
No affiliation info available
Winteler H
No affiliation info available
Foglino M
No affiliation info available
Lazdunski A
No affiliation info available
Haas D
No affiliation info available

MeSH

4-ButyrolactoneAmino Acid SequenceBacterial ProteinsChromosome MappingCloning, MolecularCyanidesDNA-Binding ProteinsGene Expression Regulation, BacterialLipaseMolecular Sequence DataPlasmidsPseudomonas aeruginosaPyocyanineSequence AlignmentSignal TransductionTrans-ActivatorsTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9159518

Citation

Reimmann, C, et al. "The Global Activator GacA of Pseudomonas Aeruginosa PAO Positively Controls the Production of the Autoinducer N-butyryl-homoserine Lactone and the Formation of the Virulence Factors Pyocyanin, Cyanide, and Lipase." Molecular Microbiology, vol. 24, no. 2, 1997, pp. 309-19.
Reimmann C, Beyeler M, Latifi A, et al. The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide, and lipase. Mol Microbiol. 1997;24(2):309-19.
Reimmann, C., Beyeler, M., Latifi, A., Winteler, H., Foglino, M., Lazdunski, A., & Haas, D. (1997). The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide, and lipase. Molecular Microbiology, 24(2), 309-19.
Reimmann C, et al. The Global Activator GacA of Pseudomonas Aeruginosa PAO Positively Controls the Production of the Autoinducer N-butyryl-homoserine Lactone and the Formation of the Virulence Factors Pyocyanin, Cyanide, and Lipase. Mol Microbiol. 1997;24(2):309-19. PubMed PMID: 9159518.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide, and lipase. AU - Reimmann,C, AU - Beyeler,M, AU - Latifi,A, AU - Winteler,H, AU - Foglino,M, AU - Lazdunski,A, AU - Haas,D, PY - 1997/4/1/pubmed PY - 1997/4/1/medline PY - 1997/4/1/entrez SP - 309 EP - 19 JF - Molecular microbiology JO - Mol Microbiol VL - 24 IS - 2 N2 - The global activator GacA, a highly conserved response regulator in Gram-negative bacteria, is required for the production of exoenzymes and secondary metabolites in Pseudomonas spp. The gacA gene of Pseudomonas aeruginosa PAO1 was isolated and its role in cell-density-dependent gene expression was characterized. Mutational inactivation of gacA resulted in delayed and reduced formation of the cell-density signal N-butyryl-L-homoserine lactone (BHL), of the cognate transcriptional activator RhIR (VsmR), and of the transcriptional activator LasR, which is known to positively regulate RhIR expression. Amplification of gacA on a multicopy plasmid caused precocious and enhanced production of BHL, RhIR and LasR. In parallel, the gacA gene dosage markedly influenced the BHL/RhIR-dependent formation of the cytotoxic compounds pyocyanin and cyanide and the exoenzyme lipase. However, the concentrations of another known cell-density signal of P. aeruginosa, N-oxododecanoyl-L-homoserine lactone, did not always match BHL concentrations. A model accounting for these observations places GacA function upstream of LasR and RhIR in the complex, cell-density-dependent signal-transduction pathway regulating several exoproducts and virulence factors of P. aeruginosa via BHL. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/9159518/The_global_activator_GacA_of_Pseudomonas_aeruginosa_PAO_positively_controls_the_production_of_the_autoinducer_N_butyryl_homoserine_lactone_and_the_formation_of_the_virulence_factors_pyocyanin_cyanide_and_lipase_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=1997&volume=24&issue=2&spage=309 DB - PRIME DP - Unbound Medicine ER -