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Natural 30 base pair and 69 base pair deletion variants of the LMP1 oncogene do stimulate NF-kappaB-mediated transcription.
Oncogene. 1997 May 01; 14(17):2123-6.O

Abstract

An increasing number of reports shows a link between the Epstein-Barr virus (EBV) and lymphoid neoplasia. The latent membrane protein 1 (LMP1) is likely to play a determinant role in this process since this EBV encoded protein has oncogenic properties and is usually expressed in EBV-associated lymphoproliferative diseases (LPD), except Burkitt's lymphoma. We previously identified in LPD patients mutational hot spots and a 30 bp or 69 bp deletion in the LMP1 gene region coding for the C-terminal domain. These deletions are located in an area shown to be important for the activation of the transcription factor NF-kappaB. These findings lead us to test whether these natural deletion variants may have a functional effect. We measured the stimulation of their activity using a luciferase reporter plasmid containing NF-kappaB responsive elements. We tested the NF-kappaB inducing activity of four naturally occurring LMP1 deletion variants. Our results show that these deletion variants activate NF-kappaB to the same level as the wild-type form, indicating that the crucial residues for NF-kappaB activation are conserved among the variants isolated and lie within the last 32 amino acids of the C-terminal domain of the LMP1 oncogene.

Authors+Show Affiliations

Institut de Pharmacologie et Toxicologie, Université de Lausanne, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9160892

Citation

Rothenberger, S, et al. "Natural 30 Base Pair and 69 Base Pair Deletion Variants of the LMP1 Oncogene Do Stimulate NF-kappaB-mediated Transcription." Oncogene, vol. 14, no. 17, 1997, pp. 2123-6.
Rothenberger S, Bachmann E, Berger C, et al. Natural 30 base pair and 69 base pair deletion variants of the LMP1 oncogene do stimulate NF-kappaB-mediated transcription. Oncogene. 1997;14(17):2123-6.
Rothenberger, S., Bachmann, E., Berger, C., McQuain, C., Odermatt, B. F., & Knecht, H. (1997). Natural 30 base pair and 69 base pair deletion variants of the LMP1 oncogene do stimulate NF-kappaB-mediated transcription. Oncogene, 14(17), 2123-6.
Rothenberger S, et al. Natural 30 Base Pair and 69 Base Pair Deletion Variants of the LMP1 Oncogene Do Stimulate NF-kappaB-mediated Transcription. Oncogene. 1997 May 1;14(17):2123-6. PubMed PMID: 9160892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Natural 30 base pair and 69 base pair deletion variants of the LMP1 oncogene do stimulate NF-kappaB-mediated transcription. AU - Rothenberger,S, AU - Bachmann,E, AU - Berger,C, AU - McQuain,C, AU - Odermatt,B F, AU - Knecht,H, PY - 1997/5/1/pubmed PY - 1997/5/1/medline PY - 1997/5/1/entrez SP - 2123 EP - 6 JF - Oncogene JO - Oncogene VL - 14 IS - 17 N2 - An increasing number of reports shows a link between the Epstein-Barr virus (EBV) and lymphoid neoplasia. The latent membrane protein 1 (LMP1) is likely to play a determinant role in this process since this EBV encoded protein has oncogenic properties and is usually expressed in EBV-associated lymphoproliferative diseases (LPD), except Burkitt's lymphoma. We previously identified in LPD patients mutational hot spots and a 30 bp or 69 bp deletion in the LMP1 gene region coding for the C-terminal domain. These deletions are located in an area shown to be important for the activation of the transcription factor NF-kappaB. These findings lead us to test whether these natural deletion variants may have a functional effect. We measured the stimulation of their activity using a luciferase reporter plasmid containing NF-kappaB responsive elements. We tested the NF-kappaB inducing activity of four naturally occurring LMP1 deletion variants. Our results show that these deletion variants activate NF-kappaB to the same level as the wild-type form, indicating that the crucial residues for NF-kappaB activation are conserved among the variants isolated and lie within the last 32 amino acids of the C-terminal domain of the LMP1 oncogene. SN - 0950-9232 UR - https://www.unboundmedicine.com/medline/citation/9160892/Natural_30_base_pair_and_69_base_pair_deletion_variants_of_the_LMP1_oncogene_do_stimulate_NF_kappaB_mediated_transcription_ L2 - https://doi.org/10.1038/sj.onc.1201032 DB - PRIME DP - Unbound Medicine ER -