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Evaluation of scolicidal agents in an experimental hydatid disease model.

Abstract

INTRODUCTION

The treatment of hydatid disease is largely surgical, with medical treatment being reserved as coadjuvant treatment. The scolicidal agents have been, and are being used mainly during surgical manipulation of the cysts, with the object of avoiding relapses and peritoneal dissemination.

OBJECTIVE

Evaluation of the scolicidal agents used in surgery in a hydatid disease model in the mouse.

MATERIAL AND METHODS

We have used 85 Swiss OF1 mice, weighing more than 30 g, of 90 +/- 10 days of age, in which a picture of hydatid sowing was reproduced by means of intra-peritoneal inoculation with 0.2 ml of a suspension which contained approximately 1,200 viable protoscolex of Echinococcus granulosus which came from the livers of parasite-infested sheep. 24 h after the inoculation, the mice were subjected to a median laparotomy for the introduction of 1 ml of the scolicidal solution to be evaluated: physiologic saline (n = 10); 10% povidone iodine (n = 15); praziquantel (n = 15); 10% hydrogen peroxide (n = 15); 10% hypertonic saline (n = 15); simulated operation (n = 15). After 7 months of follow-up, the mice were sacrificed and the following was evaluated: number of isolated cysts, cyst masses, and total cysts.

RESULTS

The number of isolated cysts which developed was significantly lower in the hydrogen peroxide group (tF 2.14 < RC 3.29). The number of cyst masses was significantly reduced in the hydrogen peroxide group (tF 2.14 < RC 2.18), in the povidone iodine group (tF 2.17 < RC 3), and in the hypertonic saline group (tF 2.11 < RC 2.77). The total number of cysts which developed decreased significantly in the hydrogen peroxide (tF 2.14 < RC 2.84) and the povidone iodine (tF 2.17 < RC 3.79) groups.

CONCLUSIONS

Hydrogen peroxide and povidone iodine show a greater protoscolicidal effect than simple cleansing with physiological saline, hypertonic saline, or praziquantel.

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  • Authors+Show Affiliations

    ,

    Institute for Surgical Research, Doce de Octubre University Hospital, Madrid, Spain.

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    Source

    MeSH

    Animals
    Anti-Infective Agents, Local
    Antiplatyhelmintic Agents
    Disease Models, Animal
    Echinococcosis, Hepatic
    Hydrogen Peroxide
    Injections, Intraperitoneal
    Mice
    Povidone-Iodine
    Praziquantel
    Recurrence
    Sodium Chloride

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    9161837

    Citation

    Landa García, J I., et al. "Evaluation of Scolicidal Agents in an Experimental Hydatid Disease Model." European Surgical Research. Europaische Chirurgische Forschung. Recherches Chirurgicales Europeennes, vol. 29, no. 3, 1997, pp. 202-8.
    Landa García JI, Alonso E, Gonzalez-Uriarte J, et al. Evaluation of scolicidal agents in an experimental hydatid disease model. Eur Surg Res. 1997;29(3):202-8.
    Landa García, J. I., Alonso, E., Gonzalez-Uriarte, J., & Rodriguez Romano, D. (1997). Evaluation of scolicidal agents in an experimental hydatid disease model. European Surgical Research. Europaische Chirurgische Forschung. Recherches Chirurgicales Europeennes, 29(3), pp. 202-8.
    Landa García JI, et al. Evaluation of Scolicidal Agents in an Experimental Hydatid Disease Model. Eur Surg Res. 1997;29(3):202-8. PubMed PMID: 9161837.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Evaluation of scolicidal agents in an experimental hydatid disease model. AU - Landa García,J I, AU - Alonso,E, AU - Gonzalez-Uriarte,J, AU - Rodriguez Romano,D, PY - 1997/1/1/pubmed PY - 1997/1/1/medline PY - 1997/1/1/entrez SP - 202 EP - 8 JF - European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes JO - Eur Surg Res VL - 29 IS - 3 N2 - INTRODUCTION: The treatment of hydatid disease is largely surgical, with medical treatment being reserved as coadjuvant treatment. The scolicidal agents have been, and are being used mainly during surgical manipulation of the cysts, with the object of avoiding relapses and peritoneal dissemination. OBJECTIVE: Evaluation of the scolicidal agents used in surgery in a hydatid disease model in the mouse. MATERIAL AND METHODS: We have used 85 Swiss OF1 mice, weighing more than 30 g, of 90 +/- 10 days of age, in which a picture of hydatid sowing was reproduced by means of intra-peritoneal inoculation with 0.2 ml of a suspension which contained approximately 1,200 viable protoscolex of Echinococcus granulosus which came from the livers of parasite-infested sheep. 24 h after the inoculation, the mice were subjected to a median laparotomy for the introduction of 1 ml of the scolicidal solution to be evaluated: physiologic saline (n = 10); 10% povidone iodine (n = 15); praziquantel (n = 15); 10% hydrogen peroxide (n = 15); 10% hypertonic saline (n = 15); simulated operation (n = 15). After 7 months of follow-up, the mice were sacrificed and the following was evaluated: number of isolated cysts, cyst masses, and total cysts. RESULTS: The number of isolated cysts which developed was significantly lower in the hydrogen peroxide group (tF 2.14 < RC 3.29). The number of cyst masses was significantly reduced in the hydrogen peroxide group (tF 2.14 < RC 2.18), in the povidone iodine group (tF 2.17 < RC 3), and in the hypertonic saline group (tF 2.11 < RC 2.77). The total number of cysts which developed decreased significantly in the hydrogen peroxide (tF 2.14 < RC 2.84) and the povidone iodine (tF 2.17 < RC 3.79) groups. CONCLUSIONS: Hydrogen peroxide and povidone iodine show a greater protoscolicidal effect than simple cleansing with physiological saline, hypertonic saline, or praziquantel. SN - 0014-312X UR - https://www.unboundmedicine.com/medline/citation/9161837/Evaluation_of_scolicidal_agents_in_an_experimental_hydatid_disease_model_ L2 - https://www.karger.com?DOI=10.1159/000129525 DB - PRIME DP - Unbound Medicine ER -