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Differential effects of pharmacologically generated reactive oxygen species upon functional activity of epididymal mouse spermatozoa.
Can J Physiol Pharmacol 1997; 75(3):173-8CJ

Abstract

Several studies indicate that reactive oxygen species (ROS) are involved in defective sperm function pathophysiology. In this study we attempted to determine differentially the effects of xanthine (0.12 mM) plus xanthine oxidase (0.035 U/mL) (X+XO, a ROS promoter system), ROS scavengers (Tiron (TIR, 15 mM); catalase (CAT, 10 micrograms/mL); dimethylsulfoxide (DMSO, 140 mM)), and X+XO plus scavengers on several epididymal mouse spermatozoa functional parameters, incubated in NTPC medium, for 29 min. In the presence of X+XO, progressive gametes significantly diminished. TIR or CAT attenuated this effect, but DMSO did not. Inversely, X+XO increased the bending-forms population; only TIR reversed this phenomenon. The ROS promoter system diminished the viable cell population; all scavengers assayed maintained sperm viability at levels similar to control ones. When exposed to hypoosmotic shock after 29 min incubation with X+XO, the percentage of swollen cells decreased; TIR, CAT, or DMSO did not prevent this effect. Our experiments demonstrate that it is possible to differentiate the deleterious ROS effects upon sperm functional activity. O-2. and H2O2 preferentially seem to modify sperm motility, O-2. exhibiting the greatest ability for generating bending-form gametes, OH-being the most lethal ROS. In addition, sperm membrane clearly appears as the most damaged structure.

Authors+Show Affiliations

Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Santa Rosa, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9164698

Citation

Baiardi, G, et al. "Differential Effects of Pharmacologically Generated Reactive Oxygen Species Upon Functional Activity of Epididymal Mouse Spermatozoa." Canadian Journal of Physiology and Pharmacology, vol. 75, no. 3, 1997, pp. 173-8.
Baiardi G, Ruiz RD, Fiol de Cuneo M, et al. Differential effects of pharmacologically generated reactive oxygen species upon functional activity of epididymal mouse spermatozoa. Can J Physiol Pharmacol. 1997;75(3):173-8.
Baiardi, G., Ruiz, R. D., Fiol de Cuneo, M., Ponce, A. A., Lacuara, J. L., & Vincenti, L. (1997). Differential effects of pharmacologically generated reactive oxygen species upon functional activity of epididymal mouse spermatozoa. Canadian Journal of Physiology and Pharmacology, 75(3), pp. 173-8.
Baiardi G, et al. Differential Effects of Pharmacologically Generated Reactive Oxygen Species Upon Functional Activity of Epididymal Mouse Spermatozoa. Can J Physiol Pharmacol. 1997;75(3):173-8. PubMed PMID: 9164698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential effects of pharmacologically generated reactive oxygen species upon functional activity of epididymal mouse spermatozoa. AU - Baiardi,G, AU - Ruiz,R D, AU - Fiol de Cuneo,M, AU - Ponce,A A, AU - Lacuara,J L, AU - Vincenti,L, PY - 1997/3/1/pubmed PY - 1997/3/1/medline PY - 1997/3/1/entrez SP - 173 EP - 8 JF - Canadian journal of physiology and pharmacology JO - Can. J. Physiol. Pharmacol. VL - 75 IS - 3 N2 - Several studies indicate that reactive oxygen species (ROS) are involved in defective sperm function pathophysiology. In this study we attempted to determine differentially the effects of xanthine (0.12 mM) plus xanthine oxidase (0.035 U/mL) (X+XO, a ROS promoter system), ROS scavengers (Tiron (TIR, 15 mM); catalase (CAT, 10 micrograms/mL); dimethylsulfoxide (DMSO, 140 mM)), and X+XO plus scavengers on several epididymal mouse spermatozoa functional parameters, incubated in NTPC medium, for 29 min. In the presence of X+XO, progressive gametes significantly diminished. TIR or CAT attenuated this effect, but DMSO did not. Inversely, X+XO increased the bending-forms population; only TIR reversed this phenomenon. The ROS promoter system diminished the viable cell population; all scavengers assayed maintained sperm viability at levels similar to control ones. When exposed to hypoosmotic shock after 29 min incubation with X+XO, the percentage of swollen cells decreased; TIR, CAT, or DMSO did not prevent this effect. Our experiments demonstrate that it is possible to differentiate the deleterious ROS effects upon sperm functional activity. O-2. and H2O2 preferentially seem to modify sperm motility, O-2. exhibiting the greatest ability for generating bending-form gametes, OH-being the most lethal ROS. In addition, sperm membrane clearly appears as the most damaged structure. SN - 0008-4212 UR - https://www.unboundmedicine.com/medline/citation/9164698/Differential_effects_of_pharmacologically_generated_reactive_oxygen_species_upon_functional_activity_of_epididymal_mouse_spermatozoa_ L2 - http://www.nrcresearchpress.com/doi/full/10.1139/cjpp-75-3-173?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -