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An ATP-dependent inwardly rectifying potassium channel, KAB-2 (Kir4. 1), in cochlear stria vascularis of inner ear: its specific subcellular localization and correlation with the formation of endocochlear potential.
J Neurosci. 1997 Jun 15; 17(12):4711-21.JN

Abstract

Cochlear endolymph has a highly positive potential of approximately +80 mV. This so-called endocochlear potential (EP) is essential for hearing. Although pivotal roles of K+ channels in the formation of EP have been suggested, the types and distribution of K+ channels in cochlea have not been characterized. Because EP was depressed by vascular perfusion of Ba2+, an inhibitor of inwardly rectifying K+ (Kir) channels, but not by either 4-aminopyridine or tetraethylammonium, we examined the expression of Kir channel subunits in cochlear stria vascularis, the tissue that is supposed to play the central role in the generation of positive EP. Of 11 members of the Kir channel family examined with reverse transcription-PCR, we could detect only expression of KAB-2 (Kir4.1) mRNA in stria vascularis. KAB-2 immunoreactivity was specifically localized at the basolateral membrane of marginal cells but not in either basal or intermediate cells. Developmental expression of KAB-2 in marginal cells paralleled formation of EP. Furthermore, deaf mutant mice (viable dominant spotting; WV/WV) expressed no KAB-2 in their marginal cells. These results suggest that KAB-2 in marginal cells may be critically involved in the generation of positive EP.

Authors+Show Affiliations

Department of Pharmacology II, Faculty of Medicine, Osaka University, Osaka 565, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9169531

Citation

Hibino, H, et al. "An ATP-dependent Inwardly Rectifying Potassium Channel, KAB-2 (Kir4. 1), in Cochlear Stria Vascularis of Inner Ear: Its Specific Subcellular Localization and Correlation With the Formation of Endocochlear Potential." The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, vol. 17, no. 12, 1997, pp. 4711-21.
Hibino H, Horio Y, Inanobe A, et al. An ATP-dependent inwardly rectifying potassium channel, KAB-2 (Kir4. 1), in cochlear stria vascularis of inner ear: its specific subcellular localization and correlation with the formation of endocochlear potential. J Neurosci. 1997;17(12):4711-21.
Hibino, H., Horio, Y., Inanobe, A., Doi, K., Ito, M., Yamada, M., Gotow, T., Uchiyama, Y., Kawamura, M., Kubo, T., & Kurachi, Y. (1997). An ATP-dependent inwardly rectifying potassium channel, KAB-2 (Kir4. 1), in cochlear stria vascularis of inner ear: its specific subcellular localization and correlation with the formation of endocochlear potential. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, 17(12), 4711-21.
Hibino H, et al. An ATP-dependent Inwardly Rectifying Potassium Channel, KAB-2 (Kir4. 1), in Cochlear Stria Vascularis of Inner Ear: Its Specific Subcellular Localization and Correlation With the Formation of Endocochlear Potential. J Neurosci. 1997 Jun 15;17(12):4711-21. PubMed PMID: 9169531.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An ATP-dependent inwardly rectifying potassium channel, KAB-2 (Kir4. 1), in cochlear stria vascularis of inner ear: its specific subcellular localization and correlation with the formation of endocochlear potential. AU - Hibino,H, AU - Horio,Y, AU - Inanobe,A, AU - Doi,K, AU - Ito,M, AU - Yamada,M, AU - Gotow,T, AU - Uchiyama,Y, AU - Kawamura,M, AU - Kubo,T, AU - Kurachi,Y, PY - 1997/6/15/pubmed PY - 1997/6/15/medline PY - 1997/6/15/entrez SP - 4711 EP - 21 JF - The Journal of neuroscience : the official journal of the Society for Neuroscience JO - J Neurosci VL - 17 IS - 12 N2 - Cochlear endolymph has a highly positive potential of approximately +80 mV. This so-called endocochlear potential (EP) is essential for hearing. Although pivotal roles of K+ channels in the formation of EP have been suggested, the types and distribution of K+ channels in cochlea have not been characterized. Because EP was depressed by vascular perfusion of Ba2+, an inhibitor of inwardly rectifying K+ (Kir) channels, but not by either 4-aminopyridine or tetraethylammonium, we examined the expression of Kir channel subunits in cochlear stria vascularis, the tissue that is supposed to play the central role in the generation of positive EP. Of 11 members of the Kir channel family examined with reverse transcription-PCR, we could detect only expression of KAB-2 (Kir4.1) mRNA in stria vascularis. KAB-2 immunoreactivity was specifically localized at the basolateral membrane of marginal cells but not in either basal or intermediate cells. Developmental expression of KAB-2 in marginal cells paralleled formation of EP. Furthermore, deaf mutant mice (viable dominant spotting; WV/WV) expressed no KAB-2 in their marginal cells. These results suggest that KAB-2 in marginal cells may be critically involved in the generation of positive EP. SN - 0270-6474 UR - https://www.unboundmedicine.com/medline/citation/9169531/An_ATP_dependent_inwardly_rectifying_potassium_channel_KAB_2__Kir4__1__in_cochlear_stria_vascularis_of_inner_ear:_its_specific_subcellular_localization_and_correlation_with_the_formation_of_endocochlear_potential_ DB - PRIME DP - Unbound Medicine ER -